|VSIG2 in Mouse Stomach. VSIG2 was detected in perfusion fixed frozen sections of mouse stomach using Rat Anti-Mouse VSIG2 Monoclonal Antibody (Catalog # MAB4766) at 25 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to the plasma membranes of cells in gastric glands. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.|
VSIG2 (V-set and Ig domain-containing protein 2; also CTM and CT-like protein) is presumably a 50‑60 kDa member of the CTX family of Ig-Superfamily proteins. It shows expression in stomach and prostate by Northern blot, and likely participates in cell adhesion. Mouse VSIG2 precursor is 328 amino acids (aa) in length. It is a type I transmembrane (glyco)protein that contains a 220 aa extracellular domain (ECD) (aa 25‑244) and a 63 aa cytoplasmic region. The ECD contains one V-type (aa 25‑138) and one C2-type Ig-like domain (aa 145‑234). Over aa 25‑244, mouse VSIG2 is 94%, 83% and 85% aa identical to rat, canine and human VSIG2, respectively. Two potential splice variants exist, one that shows a deletion of aa 305‑328 and a second that shows a deletion of aa 144‑236.
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