Mouse Wnt-11 Antibody

Catalog # Availability Size / Price Qty
MAB3746
MAB3746-SP
Detection of Mouse Wnt‑11 by Western Blot.
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Mouse Wnt-11 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Wnt-11 in direct ELISAs and Western blots. In these formats, this antibody shows no cross-reactivity with recombinant human Wnt-2, -7a, -7b, recombinant mouse Wnt-1, -3a, -4, -5a, -5b, -8a, -8b, or –9a. Western blotting with human cell extracts demonstrates that this antibody also detects human Wnt-11.
Source
Monoclonal Rat IgG1 Clone # 310622
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant mouse Wnt-11
Leu39-Ala79 and Ser225-Arg297
Accession # NP_033545
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Immunohistochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse Wnt-11 antibody by Western Blot. View Larger

Detection of Mouse Wnt‑11 by Western Blot. Western blot shows lysates of mouse embryo (13 d.p.c.) tissue. PVDF Membrane was probed with 2 µg/mL of Rat Anti-Mouse Wnt-11 Monoclonal Antibody (Catalog # MAB3746) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Wnt-11 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry Wnt-11 antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr). View Larger

Wnt‑11 in Mouse Embryo. Wnt-11 was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c.) using Rat Anti-Mouse Wnt-11 Monoclonal Antibody (Catalog # MAB3746) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of muscle cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Wnt-11

Wnt-11 belongs to the Wnt family of secreted, highly conserved, cysteine-rich signaling glcoproteins that are important in vertebrate development. Mouse Wnt-11a is expressed in early development and is required for cardiogenesis and branching morphogenesis of ureters. Wnt-11 signals by a non-canonical pathway and may repress canonical signaling by other Wnts. Mouse and human Wnt‑11 shares 96% identity over the sequences expressed in the immunogen.

Long Name
Wingless-type MMTV Integration Site Family, Member 11
Entrez Gene IDs
7481 (Human); 22411 (Mouse)
Alternate Names
HWNT11; MGC141946; MGC141948; protein Wnt-11; wingless-type MMTV integration site family, member 11; Wnt11; Wnt-11

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Mouse Wnt-11 Antibody

Average Rating: 4.5 (Based on 2 Reviews)

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Mouse Wnt-11 Antibody
By Anonymous on 12/13/2021
Application: IHC Sample Tested: Colorectal cancer tissue Species: Mouse

Mouse Wnt-11 Antibody
By Anonymous on 12/09/2020
Application: ICC/IF Sample Tested: E11.5 mouse embryo fixed in 4% PFA Species: Mouse

Staining was done on E11.5 mouse embryo heart sections (Right Ventricle) (4% PFA fixed).
Concentration used - 8ug/mL.