Mouse Wnt-11 Antibody (310622)

Name: Mouse Wnt-11 Antibody (310622)
Brand: R&D Systems
SKU: MAB3746
Rating: 4.5 (2 reviews)

R&D Systems | Catalog # MAB3746

(2)

Product Documents

Discontinued Product

MAB3746 has been discontinued. View all Wnt-11 products.

Product Specifications
Scientific Data
Applications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG₁ Clone # 310622

View all Wnt-11 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse Wnt-11
Leu39-Ala79 and Ser225-Arg297
Accession # NP_033545

Specificity

Detects mouse Wnt-11 in direct ELISAs and Western blots. In these formats, this antibody shows no cross-reactivity with recombinant human Wnt-2, -7a, -7b, recombinant mouse Wnt-1, -3a, -4, -5a, -5b, -8a, -8b, or –9a. Western blotting with human cell extracts demonstrates that this antibody also detects human Wnt-11.

Clonality

Monoclonal

Host

Rat

Isotype

IgG₁

Scientific Data Images for Mouse Wnt-11 Antibody (310622)

Detection of Mouse Wnt‑11 by Western Blot.

Western blot shows lysates of mouse embryo (13 d.p.c.) tissue. PVDF Membrane was probed with 2 µg/mL of Rat Anti-Mouse Wnt-11 Monoclonal Antibody (Catalog # MAB3746) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Wnt-11 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Wnt‑11 in Mouse Embryo.

Wnt-11 was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c.) using Rat Anti-Mouse Wnt-11 Monoclonal Antibody (Catalog # MAB3746) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of muscle cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Applications for Mouse Wnt-11 Antibody (310622)

Application

Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed frozen sections of mouse embryo (15 d.p.c.)

Western Blot

2 µg/mL
Sample: Mouse embryo (13 d.p.c.) tissue

Reviewed Applications

Read 2 reviews rated 4.5 using MAB3746 in the following applications:

Immunocytochemistry/Immunofluorescence (1 Review)
Immunohistochemistry (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: Wnt-11

Wnt-11 belongs to the Wnt family of secreted, highly conserved, cysteine-rich signaling glcoproteins that are important in vertebrate development. Mouse Wnt-11a is expressed in early development and is required for cardiogenesis and branching morphogenesis of ureters. Wnt-11 signals by a non-canonical pathway and may repress canonical signaling by other Wnts. Mouse and human Wnt‑11 shares 96% identity over the sequences expressed in the immunogen.

Long Name

Wingless-type MMTV Integration Site Family, Member 11

Alternate Names

Wnt11

Entrez Gene IDs

7481 (Human); 22411 (Mouse)

Gene Symbol

WNT11

UniProt

NP_033545

Additional Wnt-11 Products

Product Documents for Mouse Wnt-11 Antibody (310622)

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Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse Wnt-11 Antibody (310622)

For research use only

Related Research Areas

Wnt Ligands (Wnts)

Customer Reviews for Mouse Wnt-11 Antibody (310622) (2)

4.5 out of 5

2 Customer Ratings

5 Stars

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4 Stars

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1 Stars

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Customer Images

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Mouse Wnt-11 Antibody

Name: Anonymous

Application: Immunohistochemistry

Sample Tested: Colorectal cancer tissue

Species: Mouse

Verified Customer | Posted 12/13/2021
Mouse Wnt-11 Antibody

Name: Anonymous

Application: Immunocytochemistry/Immunofluorescence

Sample Tested: E11.5 mouse embryo fixed in 4% PFA

Species: Mouse

Verified Customer | Posted 12/09/2020

Staining was done on E11.5 mouse embryo heart sections (Right Ventricle) (4% PFA fixed). Concentration used - 8ug/mL.

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