Key Product Details

Species Reactivity

Mouse

Applications

Western Blot

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all Wnt-2b Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse Wnt‑2b
Leu64-Glu104 and Arg251-Ala312
Accession # O70283

Specificity

Detects mouse Wnt-2b in Western blots. In Western blots, approximately 25% cross-reactivity with recombinant human (rh) Wnt-2 is observed and less than 5% cross-reactivity with rhWnt-7a, rhWnt-9a, recombinant mouse (rm) Wnt-1, rmWnt-3a, rmWnt-4, rmWnt-5a, rmWnt-5b, rmWnt-8a, rmWnt-8b, rmWnt-9b, rmWnt-10a, rmWnt-10b, rmWnt-11, and rmWnt-16 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Mouse Wnt‑2b Biotinylated Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse Wnt‑2b

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Wnt-2b

Mouse Wnt-2b (also Wnt-13) is a 389 amino acid, 42kDa member of the large and highly conserved Wnt family of secreted, cysteine-rich glycoprotein signaling molecules. Wnt proteins are associated with developmental and carcinogenic processes. Wnt-2b may be necessary to maintain stem cells in an undifferentiated state. As a type II transmembrane protein with a long C-terminal extracellular and a short N-terminal cytoplasmic domain, Wnt-2b is presumably cleaved within its extracellular region to release the secreted form. In the region from aa 64‑104, mouse Wnt-2b is 100% identical to the human and rat protein, and over the 251‑312 aa region also used as immunogen, mouse Wnt-2b is 97% and 100% identical to the corresponding human and rat sequences, respectively.

Long Name

Wingless-type MMTV Integration Site Family Member 2b

Alternate Names

Wnt-13, Wnt2b, XWNT2

Entrez Gene IDs

7482 (Human); 22414 (Mouse); 116466 (Rat)

Gene Symbol

WNT2B

UniProt

O70283

Additional Wnt-2b Products

Product Documents for Mouse Wnt‑2b Biotinylated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse Wnt‑2b Biotinylated Antibody

For research use only

Related Research Areas

Wnt Ligands (Wnts)

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