Mouse YM1/Chitinase 3-like 3 Antibody

(4 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse YM1/Chitinase 3-like in direct ELISAs and Western blots.
  • Source
    Monoclonal Rat IgG2A Clone # 281926
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse YM1/Chitinase 3-like
    Tyr22-Tyr398
    Accession # O35744
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.5 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Mouse YM1/Chitinase 3-like 3 by Western Blot. Western blot shows lysates of mouse bone marrow. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Mouse YM1/Chitinase 3-like 3 Monoclonal Antibody (Catalog # MAB2446) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for YM1/Chitinase 3-like 3 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: YM1/Chitinase 3-like 3
Eosinophil Chemotactic Factor L (ECF-L), also known as YM1, is a secreted lectin that binds heparin and G1cN oligomers. It is produced primarily by macrophages during inflammation. ECF-L shares homology with chitinase family proteins but lacks enzymatic activity.
  • Entrez Gene IDs:
    12655 (Mouse)
  • Alternate Names:
    AI505981; CHI3L3; Chitinase 3 like 3; Chitinase 3-like 3; ECF-L; YM1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
Filter your results:

Species
Applications
Sample Type
  1. IkappaB kinase activity drives fetal lung macrophage maturation along a non-M1/M2 paradigm.
    Authors: Stouch A, Zaynagetdinov R, Barham W, Stinnett A, Slaughter J, Yull F, Hoffman H, Blackwell T, Prince L
    J Immunol, 2014;193(3):1184-93.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  2. Protective immunity against pulmonary cryptococcosis is associated with STAT1-mediated classical macrophage activation.
    Authors: Hardison S, Herrera G, Young M, Hole C, Wozniak K, Wormley F
    J Immunol, 2012;189(8):4060-8.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  3. Central nervous system fibrosis is associated with fibrocyte-like infiltrates.
    Authors: Aldrich A, Kielian T
    Am. J. Pathol., 2011;179(6):2952-62.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  4. p47phox deficiency induces macrophage dysfunction resulting in progressive crystalline macrophage pneumonia.
    Authors: Liu Q, Cheng LI, Yi L, Zhu N, Wood A, Changpriroa CM, Ward JM, Jackson SH
    Am. J. Pathol., 2009;174(1):153-63.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2A Isotype Control

Ctrl MAB006 90  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Normal Rat IgG Control

Ctrl 6-001-A 9
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Normal Rat IgG Control (Azide Free)

Ctrl 6-001-F 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rat IgG2A Isotype Control

Ctrl MAB006R  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Secondary Antibodies
Description Application Cat# Citations Images  

Rat IgG HRP-conjugated Antibody

WB, Simple Western HAF005 7  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat Anti-Rat F(ab)2 IgG (H+L)
APC-conjugated Antibody

Flow F0113 2  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat Anti-Rat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL013 2
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rat F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0105B 2  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat Anti-Rat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL015 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat Anti-Rat IgG Biotinylated Antibody

WB BAF005 2
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat Anti-Rat IgG Antibody

WB AF005 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rat IgG VisUCyte HRP Polymer Antibody

IHC VC005  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rat F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0104B  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat Anti-Rat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL014
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat Anti-Rat F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0115 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Have you used Mouse YM1/Chitinase 3-like 3 Antibody?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

Contact Information

Calculators

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷
R&D Systems Guarantee

Monoclonal
Antibody
Panels For
Screening

Learn More
Monoclonal Antibodies

Feedback

Customer Information