Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot

Cited:

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 281926
View all YM1/Chitinase 3-like 3 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse YM1/Chitinase 3-like
Tyr22-Tyr398
Accession # O35744

Specificity

Detects mouse YM1/Chitinase 3-like in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Mouse YM1/Chitinase 3-like 3 Antibody

Detection of Mouse YM1/Chitinase 3-like 3 antibody by Western Blot.

Detection of Mouse YM1/Chitinase 3-like 3 by Western Blot.

Western blot shows lysates of mouse bone marrow. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Mouse YM1/Chitinase 3-like 3 Monoclonal Antibody (Catalog # MAB2446) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for YM1/Chitinase 3-like 3 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Mouse YM1/Chitinase 3-like 3 Antibody

Application
Recommended Usage

Western Blot

0.5 µg/mL
Sample: Mouse bone marrow

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: YM1/Chitinase 3-like 3

Eosinophil Chemotactic Factor L (ECF-L), also known as YM1, is a secreted lectin that binds heparin and G1cN oligomers. It is produced primarily by macrophages during inflammation. ECF-L shares homology with chitinase family proteins but lacks enzymatic activity.

Alternate Names

CHI3L3, Chitinase 3 like 3, Chitinase 3-like 3, ECF-L

Entrez Gene IDs

12655 (Mouse)

Gene Symbol

CHIL3

UniProt

O35744

Additional YM1/Chitinase 3-like 3 Products

Product Documents for Mouse YM1/Chitinase 3-like 3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Mouse YM1/Chitinase 3-like 3 Antibody

For research use only

Citations for Mouse YM1/Chitinase 3-like 3 Antibody

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