Detection of Mouse YM1/Chitinase 3-like 3 by Western Blot.
Western blot shows lysates of mouse bone marrow. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Mouse YM1/Chitinase 3-like 3 Monoclonal Antibody (Catalog # MAB2446) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for YM1/Chitinase 3-like 3 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: YM1/Chitinase 3-like 3
Eosinophil Chemotactic Factor L (ECF-L), also known as YM1, is a secreted lectin that binds heparin and G1cN oligomers. It is produced primarily by macrophages during inflammation. ECF-L shares homology with chitinase family proteins but lacks enzymatic activity.
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