MRPS22 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-86787

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown, Independent Antibodies

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all MRPS22 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: TKKPTFMDEEVQSILTKMTGLNLQKTFKPAIQELKPPTYKLMTQAQLEEATRQAVEAAKVRLKMPPVLEERVPINDVLAEDKILEGTETTKYVFTDISYSIPHRERFIVVREPSGTLRKA

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 23172368).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MRPS22 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: MRPS22 Antibody [NBP1-86787]

Immunocytochemistry/ Immunofluorescence: MRPS22 Antibody [NBP1-86787]

Immunocytochemistry/Immunofluorescence: MRPS22 Antibody [NBP1-86787] - Staining of human cell line U-2 OS shows localization to mitochondria. Antibody staining is shown in green.
MRPS22 Antibody - BSA Free Immunohistochemistry-Paraffin: MRPS22 Antibody - BSA Free [NBP1-86787]

Immunohistochemistry-Paraffin: MRPS22 Antibody - BSA Free [NBP1-86787]

Staining of human cerebral cortex, gastrointestinal, kidney and testis using Anti-MRPS22 antibody NBP1-86787 (A) shows similar protein distribution across tissues to independent antibody NBP1-86788 (B).
MRPS22 Antibody - BSA Free Immunohistochemistry-Paraffin: MRPS22 Antibody - BSA Free [NBP1-86787]

Immunohistochemistry-Paraffin: MRPS22 Antibody - BSA Free [NBP1-86787]

Staining of human kidney shows moderate granular cytoplasmic positivity in cells in tubules.
MRPS22 Antibody - BSA Free Immunohistochemistry-Paraffin: MRPS22 Antibody - BSA Free [NBP1-86787]

Immunohistochemistry-Paraffin: MRPS22 Antibody - BSA Free [NBP1-86787]

Staining of human testis shows moderate granular cytoplasmic positivity in cells in seminiferous ducts.
MRPS22 Antibody - BSA Free Immunohistochemistry-Paraffin: MRPS22 Antibody - BSA Free [NBP1-86787]

Immunohistochemistry-Paraffin: MRPS22 Antibody - BSA Free [NBP1-86787]

Staining of human cerebral cortex shows strong granular cytoplasmic positivity in neurons.
MRPS22 Antibody - BSA Free Immunohistochemistry-Paraffin: MRPS22 Antibody - BSA Free [NBP1-86787]

Immunohistochemistry-Paraffin: MRPS22 Antibody - BSA Free [NBP1-86787]

Staining of human small intestine shows strong granular cytoplasmic positivity in glandular cells.
MRPS22 Antibody - BSA Free Western Blot: MRPS22 Antibody - BSA Free [NBP1-86787]

Western Blot: MRPS22 Antibody - BSA Free [NBP1-86787]

Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
MRPS22 Antibody - BSA Free Western Blot: MRPS22 Antibody - BSA Free [NBP1-86787]

Western Blot: MRPS22 Antibody - BSA Free [NBP1-86787]

Analysis in Caco-2 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-MRPS22 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.

Applications for MRPS22 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
IHC reported in scientific literature (PMID: 23172368). For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MRPS22

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S21P family. Pseudogenes corresponding to this gene are found on chromosomes 1p, 1q, 9p, 10p, 10q, 16q, and 17q. Available sequence data analyses identified splice variants that differ in the 5' UTR; both transcripts encode the same protein

Alternate Names

C3orf5GK002, COXPD5, GIBT, mitochondrial ribosomal protein S22, MRP-S2228S ribosomal protein S22, mitochondrial, RPMS22, S22mt

Gene Symbol

MRPS22

Additional MRPS22 Products

Product Documents for MRPS22 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for MRPS22 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for MRPS22 Antibody - BSA Free

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Protocols

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FAQs

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