MTA2 Antibody (9A5Y7)

Novus Biologicals | Catalog # NBP3-16291

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human

Applications

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP)

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 9A5Y7 expressed in HEK293
View all MTA2 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 569-668 of human MTA2 (O94776). GVPFSANGRPLASGIRSSSQPAAKRQKLNPADAPNPVVFVATKDTRALRKALTHLEMRRAARRPNLPLKVKPTLIAVRPPVPLPAPSHPASTNEPIVLED

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MTA2 Antibody (9A5Y7)

Western Blot: MTA2 Antibody (9A5Y7) [NBP3-16291]

Western Blot: MTA2 Antibody (9A5Y7) [NBP3-16291]

Western Blot: MTA2 Antibody (9A5Y7) [NBP3-16291] - Western blot analysis of extracts of various cell lines, using MTA2 Rabbit mAb (NBP3-16291) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
Immunohistochemistry-Paraffin: MTA2 Antibody (9A5Y7) [NBP3-16291]

Immunohistochemistry-Paraffin: MTA2 Antibody (9A5Y7) [NBP3-16291]

Immunohistochemistry-Paraffin: MTA2 Antibody (9A5Y7) [NBP3-16291] - Immunohistochemistry of paraffin-embedded human colon using MTA2 Rabbit mAb (NBP3-16291) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Chromatin Immunoprecipitation: MTA2 Antibody (9A5Y7) [NBP3-16291] - Chromatin immunoprecipitation analysis of extracts of HeLa cells, using MTA2 antibody (NBP3-16291) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
Knockout Validated: MTA2 Antibody (9A5Y7) [NBP3-16291]

Western Blot: MTA2 Antibody (9A5Y7) [NBP3-16291]

Western Blot: MTA2 Antibody (9A5Y7) [NBP3-16291] - Western blot analysis of extracts of 293T cells, using MTA2 antibody (NBP3-16291) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
MTA2 Antibody (9A5Y7)

Immunohistochemistry-MTA2 Antibody (9A5Y7)-NBP3-16291-

Immunohistochemistry-MTA2 Antibody (9A5Y7)-NBP3-16291-Analysis of U-2 OS using MTA2 antibody at dilution of 1 : 100 (40x lens). Blue: DAPI for nuclear staining.
MTA2 Antibody (9A5Y7)

Chromatin Immunoprecipitation: MTA2 Antibody (9A5Y7) [NBP3-16291] -

Chromatin Immunoprecipitation: MTA2 Antibody (9A5Y7) [NBP3-16291] - Chromatin immunoprecipitation analysis of extracts of HeLa cells, using MTA2 antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Applications for MTA2 Antibody (9A5Y7)

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

1:100 - 1:500

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Immunoprecipitation

1:100 - 1:500

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MTA2

Official Gene Symbol: MTA2 Gen Bank Accession Number: NP_004730 Gene ID: 9219 (Human) Gene Map Locus: 11q12-q13.1 (human) MTA2, a homologue of human MTA1 and MTA3, is a member of highly conserved MTA gene family. It is a component of NuRD, an ATP-dependent nucleosome remodeling and histone deacetylase complex. MTA2 is a nuclear protein that interacts with HDAC1 and HDAC2 and has a functional role in chromatin remodeling and deacetylase activity. MTA2 specifically interacts with p53 and represses p53-dependant transcriptional activation, thereby regulating p53-mediated cell growth arrest and apoptosis. It also inhibits the transcriptional activity of Estrogen receptor-alpha. Northern Blot analysis detected a ubiquitous expression of MTA2.

Alternate Names

DKFZp686F2281, metastasis associated 1 family, member 2, metastasis -associated gene 1-like 1, metastasis associated gene family, member 2, Metastasis-associated 1-like 1, metastasis-associated protein 2, metastasis-associated protein MTA2, MTA1-L1 protein, MTA1L1MTA1-L1, p53 target protein in deacetylase complex, PID

Gene Symbol

MTA2

Additional MTA2 Products

Product Documents for MTA2 Antibody (9A5Y7)

Certificate of Analysis

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Product Specific Notices for MTA2 Antibody (9A5Y7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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