MTA2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-82615

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all MTA2 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: ECSIRLPKAAKTPLKIHPLVRLPLATIVKDLVAQAPLKPKTPRGTKTPINRNQLSQNRGLGGIMVKRAYETMAGAGVPFSANGRPLASGIRSSSQPAAKRQKLNPADAPNPVVFVATKDTRALRKALTHLEMRRAARRPNLPLKVKPT

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MTA2 Antibody - BSA Free

Immunohistochemistry-Paraffin: MTA2 Antibody [NBP1-82615]

Immunohistochemistry-Paraffin: MTA2 Antibody [NBP1-82615]

Immunohistochemistry-Paraffin: MTA2 Antibody [NBP1-82615] - Analysis in human skin and liver tissues. Corresponding MTA2 RNA-seq data are presented for the same tissues.
Western Blot: MTA2 Antibody [NBP1-82615]

Western Blot: MTA2 Antibody [NBP1-82615]

Western Blot: MTA2 Antibody [NBP1-82615] - Analysis of mouse cell line NIH-3T3 and rat cell line NBT-II
Immunocytochemistry/ Immunofluorescence: MTA2 Antibody [NBP1-82615]

Immunocytochemistry/ Immunofluorescence: MTA2 Antibody [NBP1-82615]

Immunocytochemistry/Immunofluorescence: MTA2 Antibody [NBP1-82615] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: MTA2 Antibody [NBP1-82615]

Immunohistochemistry-Paraffin: MTA2 Antibody [NBP1-82615]

Immunohistochemistry-Paraffin: MTA2 Antibody [NBP1-82615] - Staining of human tonsil shows strong nuclear positivity in germinal center cells.
Western Blot: MTA2 Antibody [NBP1-82615]

Western Blot: MTA2 Antibody [NBP1-82615]

Western Blot: MTA2 Antibody [NBP1-82615] - Analysis in human cell line MOLT-4.
Immunohistochemistry-Paraffin: MTA2 Antibody [NBP1-82615]

Immunohistochemistry-Paraffin: MTA2 Antibody [NBP1-82615]

Immunohistochemistry-Paraffin: MTA2 Antibody [NBP1-82615] - Staining of human liver shows very weak nuclear positivity in hepatocytes.
Immunohistochemistry-Paraffin: MTA2 Antibody [NBP1-82615]

Immunohistochemistry-Paraffin: MTA2 Antibody [NBP1-82615]

Immunohistochemistry-Paraffin: MTA2 Antibody [NBP1-82615] - Staining of human placenta shows strong nuclear positivity in trophoblastic cells.
Immunohistochemistry-Paraffin: MTA2 Antibody [NBP1-82615]

Immunohistochemistry-Paraffin: MTA2 Antibody [NBP1-82615]

Immunohistochemistry-Paraffin: MTA2 Antibody [NBP1-82615] - Staining of human skin shows strong nuclear positivity in squamous epithelial cells.

Applications for MTA2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MTA2

Official Gene Symbol: MTA2 Gen Bank Accession Number: NP_004730 Gene ID: 9219 (Human) Gene Map Locus: 11q12-q13.1 (human) MTA2, a homologue of human MTA1 and MTA3, is a member of highly conserved MTA gene family. It is a component of NuRD, an ATP-dependent nucleosome remodeling and histone deacetylase complex. MTA2 is a nuclear protein that interacts with HDAC1 and HDAC2 and has a functional role in chromatin remodeling and deacetylase activity. MTA2 specifically interacts with p53 and represses p53-dependant transcriptional activation, thereby regulating p53-mediated cell growth arrest and apoptosis. It also inhibits the transcriptional activity of Estrogen receptor-alpha. Northern Blot analysis detected a ubiquitous expression of MTA2.

Alternate Names

DKFZp686F2281, metastasis associated 1 family, member 2, metastasis -associated gene 1-like 1, metastasis associated gene family, member 2, Metastasis-associated 1-like 1, metastasis-associated protein 2, metastasis-associated protein MTA2, MTA1-L1 protein, MTA1L1MTA1-L1, p53 target protein in deacetylase complex, PID

Gene Symbol

MTA2

Additional MTA2 Products

Product Documents for MTA2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for MTA2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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