MTAP Antibody (2G4) - Azide and BSA Free

Novus Biologicals | Catalog # H00004507-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Paraffin, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2G4

Format

Azide and BSA Free
View all MTAP Primary Antibodies »

Product Specifications

Immunogen

MTAP (AAH18625, 1 a.a. ~ 154 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MASGTTTTAVKIGIIGGTGLDDPEILEGRTEKYVDTPFGKPSDALILGKIKNVDCILLARHGRQHTIMPSKVNYQANIWALKEEGCTHVIVTTACGSLREEIQPGDIVIIDQFIDSHVRRACFPFTFHHDCFQRPPQKPSRSHCVSCATCRTMS

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Localization

Cytoplasmic

Specificity

MTAP - methylthioadenosine phosphorylase

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for MTAP Antibody (2G4) - Azide and BSA Free

Western Blot: MTAP Antibody (2G4) [H00004507-M01]

Western Blot: MTAP Antibody (2G4) [H00004507-M01]

Western Blot: MTAP Antibody (2G4) [H00004507-M01] - MTAP monoclonal antibody (M01), clone 2G4. Analysis of MTAP expression in NIH/3T3.
Immunocytochemistry/ Immunofluorescence: MTAP Antibody (2G4) [H00004507-M01]

Immunocytochemistry/ Immunofluorescence: MTAP Antibody (2G4) [H00004507-M01]

Immunocytochemistry/Immunofluorescence: MTAP Antibody (2G4) [H00004507-M01] - Analysis of monoclonal antibody to MTAP on HeLa cell. Antibody concentration 10 ug/ml.
Western Blot: MTAP Antibody (2G4) [H00004507-M01]

Western Blot: MTAP Antibody (2G4) [H00004507-M01]

Western Blot: MTAP Antibody (2G4) [H00004507-M01] - MTAP monoclonal antibody (M01), clone 2G4 Analysis of MTAP expression in HeLa.
Western Blot: MTAP Antibody (2G4) [H00004507-M01]

Western Blot: MTAP Antibody (2G4) [H00004507-M01]

Western Blot: MTAP Antibody (2G4) [H00004507-M01] - MTAP monoclonal antibody (M01), clone 2G4. Analysis of MTAP expression in PC-12.
Western Blot: MTAP Antibody (2G4) [H00004507-M01]

Western Blot: MTAP Antibody (2G4) [H00004507-M01]

Western Blot: MTAP Antibody (2G4) [H00004507-M01] - MTAP monoclonal antibody (M01), clone 2G4. Analysis of MTAP expression in Raw 264.7.
ELISA: MTAP Antibody (2G4) [H00004507-M01]

ELISA: MTAP Antibody (2G4) [H00004507-M01]

ELISA: MTAP Antibody (2G4) [H00004507-M01] - Detection limit for recombinant GST tagged MTAP is approximately 0.3ng/ml as a capture antibody.

Applications for MTAP Antibody (2G4) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF and ELISA. Use in Immunohistochemistry-Paraffin reported in scientific literature (PMID:32284171).

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: MTAP

This gene encodes an enzyme that plays a major role in polyamine metabolism and is important for the salvage of both adenine and methionine. The encoded enzyme is deficient in many cancers because this gene and the tumor suppressor p16 gene are co-deleted. Multiple alternatively spliced transcript variants have been described for this gene, but their full-length natures remain unknown.

Long Name

5'-Methylthioadenosine Phosphorylase

Alternate Names

BDMF, C86fus, DMSFH, DMSMFH, HEL-249, LGMBF, MTAPase

Entrez Gene IDs

4507 (Human)

Gene Symbol

MTAP

OMIM

156540 (Human)

UniProt

AAH18625 (Human)

Additional MTAP Products

Product Documents for MTAP Antibody (2G4) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for MTAP Antibody (2G4) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Sulfation Regulators

Citations for MTAP Antibody (2G4) - Azide and BSA Free

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Protocols

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FAQs

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