mTOR Pathway Antibody Pack
Novus Biologicals | Catalog # NBP3-20207
Loading...
Key Product Details
Species
Human, Mouse
Applications
Immunohistochemistry, Western Blot
Product Summary for mTOR Pathway Antibody Pack
This pack contains 1 vial each of: AF15371-SP (25 ug), AF15371-SP (0.05 ml), NBP2-24837SS (25 ug), AF4004-SP (25 ug), MAB1665-SP (0.025 ml), NBP1-49674SS (1 mL), HAF008 (1 mL), HAF007 (1 mL), HAF005 (1 mL), HAF017 (0.025 ml).
Loading...
Product Specifications
Application Notes
See individual datasheets of components for their validated applications
Reactivity Notes
See individual datasheets of components for their validated species
Scientific Data Images for mTOR Pathway Antibody Pack
Detection of Raptor in 293 and NIH3T3 lysates by Western Blot.
Western blot shows lysates of human 293 and mouse NIH3T3 lysate. Raptor was detected by Rabbit Anti-Human Raptor Polyclonal Antibody (Catalog # NBP2-24837) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody. This experiment was conducted under reducing conditions.
Detection of Rictor in HeLa by Flow Cytometry.
An intracellular stain was performed on HeLa cells with Rictor Antibody (7B3) NBP1-51645PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.
Detection of G beta L (GBL) in Human Prostate by Immunohistochemistry (IHC-P).
G beta L was detected in immersion fixed paraffin-embedded sections of human prostate using Goat Anti-Human/Mouse/Rat G beta L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4004) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Phospho-TOR (S2448) in Camptothecin (CPT) treated MCF-7 lysate by Western Blot.
Western blot shows lysates of MCF-7 human breast cancer cell line treated (+) with 1 µM Camptothecin (CPT) for 5 hours. PVDF membrane was treated (+) or untreated (-) with 300 U/mL CIP for 1 hour and probed with 1 µg/mL of Rat Anti-Human Phospho-TOR (S2448) Monoclonal Antibody (Catalog # MAB1665) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Phospho-TOR (S2448) at approximately 280 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of DEPTOR/DEPDC6 in Human and Mouse Liver by Western Blot.
Western blot shows lysates of (A) human liver and (B) mouse liver. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human DEPTOR/DEPDC6 Antigen Affinity-purified Polyclonal Antibody (Catalog # NBP1-49674) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody. This experiment was conducted under reducing conditions.Kit Contents for mTOR Pathway Antibody Pack
- AF15371-SP
- AF4004-SP
- HAF017: Goat IgG HRP-conjugated Antibody
- MAB1665-SP
- HAF007: Mouse IgG Horseradish Peroxidase-conjugated Antibody
- NBP1-49674SS
- NBP1-51645SS
- NBP2-24837SS
- HAF008: Rabbit IgG Horseradish Peroxidase-conjugated Antibody
- HAF005: Rat IgG Horseradish Peroxidase-conjugated Antibody
Formulation, Preparation, and Storage
Concentration
Concentration of individual antibodies may be found on the vial label. If unlisted please contact technical services.
Storage
Storage is content dependent.
Product Documents for mTOR Pathway Antibody Pack
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for mTOR Pathway Antibody Pack
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Packs are guaranteed for 1 year from date of receipt.
Customer Reviews for mTOR Pathway Antibody Pack
There are currently no reviews for this product. Be the first to review mTOR Pathway Antibody Pack and earn rewards!
Have you used mTOR Pathway Antibody Pack?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
Loading...