MUC1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-60046

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Bovine, Equine, Goat, Guinea Pig, Rabbit

Cited:

Human, Mouse, Goat, Rabbit

Predicted:

Primate (94%). Backed by our 100% Guarantee.

Applications

Validated:

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all MUC-1 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptides corresponding to a C terminal portion of the human MUC1 (between amino acids 1200-1250) [UniProt P15941].

Reactivity Notes

Goat reactivity reported in scientific literature (PMID: 28740504). Predicted to react with bovine (82%) and lar gibbon (94%) based on immunogen sequence similarity.

Specificity

The immunogen for this antibody has 100% homology to human MUC1 isoforms S2, E2, 9, F, T10, 5, Y, Y-LSP, 8, 4, 3, 2, 1 and J13.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

122 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MUC1 Antibody - BSA Free

Knockout Validated: MUC1 Antibody - BSA Free [NBP1-60046]

Western Blot: MUC1 Antibody - BSA Free [NBP1-60046]

MUC1-Antibody-Knockout-Validated-NBP1-60046-img0012.jpg
Knockout Validated: MUC1 Antibody - BSA Free [NBP1-60046]

Knockout Validated: MUC1 Antibody - BSA Free [NBP1-60046]

MUC1-Antibody-Knockout-Validated-NBP1-60046-img0013.jpg
Western Blot: MUC1 AntibodyBSA Free [NBP1-60046]

Western Blot: MUC1 AntibodyBSA Free [NBP1-60046]

Western Blot: MUC-1 Antibody [NBP1-60046] - Total protein from mouse 3T3 cells and human HeLa cells, stomach and colon was separated on a 4-15% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-MUC-1 in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.
Immunocytochemistry/ Immunofluorescence: MUC1 Antibody - BSA Free [NBP1-60046]

Immunocytochemistry/ Immunofluorescence: MUC1 Antibody - BSA Free [NBP1-60046]

Immunocytochemistry/Immunofluorescence: MUC1 Antibody [NBP1-60046] - Tested in HeLa cells at a 1:50 dilution against DyLight 488 (Green). Cells were counterstained for alpha-tubulin against DyLight 550 (Red) and mounted in DAPI fluoromount (Blue).
Immunohistochemistry-Paraffin: MUC1 Antibody - BSA Free [NBP1-60046]

Immunohistochemistry-Paraffin: MUC1 Antibody - BSA Free [NBP1-60046]

Immunohistochemistry-Paraffin: MUC1 Antibody [NBP1-60046] - Human kidney lysate tissue at an antibody concentration of 4-8ug/ml.
Immunohistochemistry-Paraffin: MUC1 Antibody - BSA Free [NBP1-60046]

Immunohistochemistry-Paraffin: MUC1 Antibody - BSA Free [NBP1-60046]

Immunohistochemistry-Paraffin: MUC1 Antibody [NBP1-60046] - Pig stomach.
Immunohistochemistry-Paraffin: MUC1 Antibody - BSA Free [NBP1-60046]

Immunohistochemistry-Paraffin: MUC1 Antibody - BSA Free [NBP1-60046]

Immunohistochemistry-Paraffin: MUC1 Antibody [NBP1-60046] - Pig stomach.
Immunohistochemistry-Paraffin: MUC1 Antibody - BSA Free [NBP1-60046]

Immunohistochemistry-Paraffin: MUC1 Antibody - BSA Free [NBP1-60046]

Immunohistochemistry-Paraffin: MUC1 Antibody [NBP1-60046] - Human stomach.
MUC1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: MUC1 Antibody - BSA Free [NBP1-60046] -

Immunocytochemistry/ Immunofluorescence: MUC1 Antibody - BSA Free [NBP1-60046] - Dexamethasone induces Muc1-CT nuclear translocation & co-localization with GR alpha in bronchial epithelium of WT mice but not in MUC1 KO mice. C57BL/6 Muc1 KO mice & WT mice were undergoing dexamethasone 10 mg/kg/day (orally) for 6 days. Animals were sacrificed at day 6 & lungs were fixed in paraformaldehyde (4%) for 48 h & embedded in Tissue-Tek® OCT™ cryosectioning compound. Lung slices were immunostained with MUC1-cytoplasmic tail (CT) & glucocorticoid receptor alpha (GR alpha ) antibodies with rhodamine/fluorescein secondary antibodies. DAPI was used to mark cell nucleus. Representative con-focal images are showed. Scale bar: 5 μm Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30458870), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
MUC1 Antibody - BSA Free

Chromatin Immunoprecipitation: MUC1 Antibody - BSA Free [NBP1-60046] -

Chromatin Immunoprecipitation: MUC1 Antibody - BSA Free [NBP1-60046] - MUC1 binds RIPK1 to mediate the RIPK1/RIPK3 pathway. (a,b) MUC1 knockdown by MUC1‐siRNA or NC‐siRNA were assayed in 16HBE cells following stimulation with TNF‐ alpha (300 ng/ml). After 24 hr of stimulation, RIPK3 & RIPK1 protein levels were measured by western blot analysis. (c,d) Normal 16HBE cells were treated with TNF‐ alpha for 24 hr, & the interaction between MUC1‐CT & RIPK1/RIPK3 were confirmed by immunoprecipitation. Data are expressed as means ± SD & were analyzed by one‐way analysis of variance, *p < 0.05. NC group (transfected with NC siRNA); MUC1‐siRNA group (transfected with MUC1‐siRNA). 16HBE: human bronchial epithelial; IB: immunoblotting; IP: immunoprecipitation; RIPK1: receptor‐interacting protein kinase‐1; RIPK3: receptor‐interacting protein kinase‐3; NC: negative control; siRNA: small interfering RNA; TNF‐ alpha : tumor necrosis factor‐ alpha Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30666647), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
MUC1 Antibody - BSA Free

Western Blot: MUC1 Antibody - BSA Free [NBP1-60046] -

Western Blot: MUC1 Antibody - BSA Free [NBP1-60046] - MUC1 downregulation & TLR4 overexpression in lung tissue from heavy smokers & COPD patients. Lung tissue from healthy (n = 10), smokers (n = 11) & COPD patients (n = 13) were analysed. MUC1 mRNA gene expression in lung tissue homogenates (a), bronchial epithelial cells (b) & sputum neutrophils (c). d MUC1-CT protein expression in lung homogenates.TLR4 mRNA gene expression in lung tissue homogenates (e), bronchial epithelial cells (f) & sputum neutrophils (g). h MUC1-CT protein expression in lung homogenates. i Correlation of TL4 & MUC1 gene expression in lung tissue from smokers & COPD patients. j Correlation of FEV1% & MUC1 gene expression in lung tissue from smokers & COPD patients. Gene expression was analyzed by real time PCR using the 2-delta Ct as described in methods. Protein expression was analyzed by western blot. Representative western blot are showed. Data are presented as median with interquartile range. “P” exact values were obtained following Kruskal Wallis test. FEV1%: forced expiratory volume in 1 s Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30458870), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
MUC1 Antibody - BSA Free

Chromatin Immunoprecipitation: MUC1 Antibody - BSA Free [NBP1-60046] -

Chromatin Immunoprecipitation: MUC1 Antibody - BSA Free [NBP1-60046] - MUC1 binds RIPK1 to mediate the RIPK1/RIPK3 pathway. (a,b) MUC1 knockdown by MUC1‐siRNA or NC‐siRNA were assayed in 16HBE cells following stimulation with TNF‐ alpha (300 ng/ml). After 24 hr of stimulation, RIPK3 & RIPK1 protein levels were measured by western blot analysis. (c,d) Normal 16HBE cells were treated with TNF‐ alpha for 24 hr, & the interaction between MUC1‐CT & RIPK1/RIPK3 were confirmed by immunoprecipitation. Data are expressed as means ± SD & were analyzed by one‐way analysis of variance, *p < 0.05. NC group (transfected with NC siRNA); MUC1‐siRNA group (transfected with MUC1‐siRNA). 16HBE: human bronchial epithelial; IB: immunoblotting; IP: immunoprecipitation; RIPK1: receptor‐interacting protein kinase‐1; RIPK3: receptor‐interacting protein kinase‐3; NC: negative control; siRNA: small interfering RNA; TNF‐ alpha : tumor necrosis factor‐ alpha Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30666647), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for MUC1 Antibody - BSA Free

Application
Recommended Usage

Flow Cytometry

reported in scientific literature (PMID 28740504)

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

4-8 ug/ml

Western Blot

0.2-1 ug/ml

Reviewed Applications

Read 1 review rated 5 using NBP1-60046 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

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  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MUC-1

Mucin 1 (MUC1), also known as episialin, EMA (epithelial membrane antigen), PEM (polymorphic epithelial mucin), and CA-15-3 antigen, is a membrane-bound type I transmembrane glycoprotein (1,2). MUC1 is typically expressed in the luminal or glandular epithelial cells of the gastrointestinal tract, breast, lungs, and more, and is often overexpressed in epithelial cancers, but also serves a protective role against infection and helps regulate inflammatory response (2,3). Human MUC1 is 1255 amino acids (aa) in length with a theoretical molecular weight of 122 kDa; however, depending on the amount of glycosylation can weigh between 250 - 500 kDa (2,4). Structurally, MUC1 consists of a N-terminal domain which contains a signal peptide, a variable number tandem repeat region (VNTR), and a SEA domain, as well a C-terminal domain which has the extracellular domain (ECD), transmembrane domain (TMD), and cytoplasmic tail (CT) (2,3). The VNTR is comprised of between 25 - 125 repeats of a 20 aa conserved sequence (3). MUC1 is heavily O-glycosylated in the VNTR and has moderate N-glycosylation sites following the VNTR and in the ECD (2). Glycosylation contributes to MUC1's functional properties (2). The MUC1 gene contains seven exons, giving rise to several MUC1 isoforms as a result of alternative splicing (2).

Overexpression of mucins, including MUC1, is a feature of many epithelial cancers (1,3,5,6). The presence of truncated glycan structures called tumor-associated carbohydrate antigens (TACAs) on MUC1 play a role in cancer progression and a loss of apical-basal polarity (5). Carbohydrate-binding partners called lectins are the primary binding partners of TACAs that give rise to the pro-tumor microenvironment and metastasis (5). Given this unique feature, TACAs are a potential target for cancer immunotherapies (5). There are a number of vaccines, drugs, and antibodies targeting MUC1 for treatment of a variety of cancers including breast, lung, and prostate (6). In addition to a role in cancer progression, MUC1, and specifically the CT portion, has been shown to have a positive, anti-inflammatory role in a variety of lung and airway infections (7).

References

1. Khodabakhsh, F., Merikhian, P., Eisavand, M. R., & Farahmand, L. (2021). Crosstalk between MUC1 and VEGF in angiogenesis and metastasis: a review highlighting roles of the MUC1 with an emphasis on metastatic and angiogenic signaling. Cancer cell international. https://doi.org/10.1186/s12935-021-01899-8

2. Nath, S., & Mukherjee, P. (2014). MUC1: a multifaceted oncoprotein with a key role in cancer progression. Trends in molecular medicine. https://doi.org/10.1016/j.molmed.2014.02.007

3. Dhar, P., & McAuley, J. (2019). The Role of the Cell Surface Mucin MUC1 as a Barrier to Infection and Regulator of Inflammation. Frontiers in cellular and infection microbiology. https://doi.org/10.3389/fcimb.2019.00117

4. Uniprot (P15941)

5. Beckwith, D. M., & Cudic, M. (2020). Tumor-associated O-glycans of MUC1: Carriers of the glyco-code and targets for cancer vaccine design. Seminars in immunology. https://doi.org/10.1016/j.smim.2020.101389

6. Almasmoum H. (2021). The Roles of Transmembrane Mucins Located on Chromosome 7q22.1 in Colorectal Cancer. Cancer management and research. https://doi.org/10.2147/CMAR.S299089

7. Ballester, B., Milara, J., & Cortijo, J. (2021). The role of mucin 1 in respiratory diseases. European respiratory review : an official journal of the European Respiratory Society. https://doi.org/10.1183/16000617.0149-2020

Long Name

Mucin 1, Cell Surface-associated

Alternate Names

CD227, Episialin, H23AG, KL-6, Mucin-1, PEM, PEMT

Entrez Gene IDs

4582 (Human)

Gene Symbol

MUC1

UniProt

Q7Z552 (Human)

Additional MUC-1 Products

Product Documents for MUC1 Antibody - BSA Free

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Product Specific Notices for MUC1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for MUC1 Antibody - BSA Free

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FAQs for MUC1 Antibody - BSA Free

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  • Q: I would like to perform a sandwich assay for the determination of MUC1 protein. May you suggest me some antibodies that can be used together? In particular, if possible, I should need antibodies product in mouse or rabbit.

    A: Unfortunately we are not aware of a pair of antibodies that have been used in sandwich ELISA in particular. If you are interested in trying two you may find our Innovators Reward Program to be helpful.

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