MUC5AC Antibody (45M1)

Novus Biologicals | Catalog # NBP2-15196

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Bovine (Negative), Chicken, Feline, Mammal, Monkey, Rabbit

Cited:

Human, Mouse, Porcine

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Flow (Intracellular), Immunocytochemistry/ Immunofluorescence, Dot Blot

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Flow Cytometry, Immunocytochemistry, Immunocytochemistry/ Immunofluorescence, Dot Blot, IF/IHC, IHC-F

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 45M1
View all MUC5AC Primary Antibodies »

Product Specifications

Immunogen

M1 mucin preparation from the fluid of an ovarian mucinous cyst belonging to an O Le(a-b) patient

Reactivity Notes

Hedgehog. Does not react with Bovine.

Use in Mouse reported in scientific literature (PMID:34260916)

Localization

Cytoplasmic

Specificity

This monoclonal antibody recognizes the peptide core of gastric mucin M1 (recently identified as Mucin 5AC).Its epitope is located in the C-terminal cysteine rich part of the peptide core of MUC5AC. Its epitope is destroyed by beta-mercaptoethanol but not by periodate treatment. This mucin is present in primary ovarian mucinous cancer but usually absent in colorectal adenocarcinoma, thus showing an expression pattern opposite to MUC2. Together with a panel of antibodies, Anti-MUC5AC may be useful for differential identification of primary mucinous ovarian tumors from colon adenocarcinoma metastatic to the ovary. MUC5AC antibodies may also be useful for identification of intestinal metaplasia as well as in the identification of pancreatic carcinoma and pre-cancerous changes vs. normal pancreas.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-32732)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for MUC5AC Antibody (45M1)

Immunohistochemistry: MUC5AC Antibody (45M1) [NBP2-15196]

Immunohistochemistry: MUC5AC Antibody (45M1) [NBP2-15196]

MUC5AC-Antibody-45M1-Immunohistochemistry-NBP2-15196-img0013.jpg
Immunohistochemistry-Paraffin: MUC5AC Antibody (45M1) [NBP2-15196]

Immunohistochemistry-Paraffin: MUC5AC Antibody (45M1) [NBP2-15196]

Immunohistochemistry-Paraffin: MUC5AC Antibody (45M1) [NBP2-15196] - Analysis using the Azide and BSA Free version of NBP2-15196. Detection Human stomach.
Immunohistochemistry-Paraffin: MUC5AC Antibody (45M1) [NBP2-15196]

Immunohistochemistry-Paraffin: MUC5AC Antibody (45M1) [NBP2-15196]

Immunohistochemistry-Paraffin: MUC5AC Antibody (45M1) [NBP2-15196] - Formalin-fixed, paraffin-embedded Rat stomach stained with MUC5AC Monoclonal Antibody (45M1).
Immunohistochemistry-Paraffin: MUC5AC Antibody (45M1) [NBP2-15196]

Immunohistochemistry-Paraffin: MUC5AC Antibody (45M1) [NBP2-15196]

Immunohistochemistry-Paraffin: MUC5AC Antibody (45M1) [NBP2-15196] - Formalin-fixed, paraffin-embedded human Gastric Carcinoma stained with MUC5AC Monoclonal Antibody (45M1).
MUC5AC Antibody (45M1)

Immunofluorescent Staining of Adult stem cell-derived Lung Organoids.

Adult stem cells isolated from human lung biopsy tissue were cultured following the steps detailed in the human lung organoid culture protocol. Lung organoids were stained with a (A) Goat Anti-Human p63/TP73L Polyclonal Antibody (Catalog # AF1916; red) and a rabbit anti-human cytokeratin 5 (KRT5) monoclonal antibody (green) to visualize basal cells; a (B) Hamster Anti-Mouse Podoplanin (PDPN) Monoclonal Antibody (Novus Biologicals, Catalog # NB600-1015; green) to visualize alveolar type I cells and a Goat Anti-Human p63/TP73L Polyclonal Antibody (Catalog # AF1916; red) to visualize basal cells; and a (C, D) Mouse Anti-MUC5AC Monoclonal Antibody (Novus Biologicals, Catalog # NBP2-15196; green) to visualize goblet cells and a Mouse Anti-Human/Mouse/Rat SOX2 Monoclonal Antibody (Catalog # MAB2018; red). All samples were counterstained with DAPI (Catalog # 5748; blue).
MUC5AC Antibody (45M1)

Dot Blot: MUC5AC Antibody (45M1) [NBP2-15196] -

Dot Blot: MUC5AC Antibody (45M1) [NBP2-15196] - Mucin MUC5AC expression & immunoreactivity in primary cells of the nasal cavity. (A) Transcription analysis (RT-PCR) of MUC5AC gene in olfactory epithelial primary cells (OEPC), respiratory epithelial primary cells (REPC), tumour cell line RPMI 2650 & the concha nasalis media (c.n. media). MUC5AC transcript signal was referenced to beta-actin transcript signal. The significance was calculated by comparison of the OEPC, REPC & RPMI 2650 data with the c.n. media transcription data using an unpaired t-test. * p < 0.05; n = 4; error bars represent mean ± SD. (B) Dot blot analysis of MUC5AC protein in lysates of OEPC, REPC, RPMI 2650 & c.n. media. All OEPC & REPC cultures shown in (A,B) were cultivated for 14 days in vitro in T flasks. (C) Immunoreactivity against MUC5AC in OEPC that were first cultured for 7 days in T flask with a minimum confluency of 70% then under ALI conditions additional 20 days. Apically secreted mucus was collected at the days indicated corresponding to a mucin production of 2 to 3 days. Statistical analysis: unpaired t-test, * p < 0.05 compared to the standard model RPMI 2650. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31374872), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
MUC5AC Antibody (45M1)

Dot Blot: MUC5AC Antibody (45M1) [NBP2-15196] -

Dot Blot: MUC5AC Antibody (45M1) [NBP2-15196] - Mucin MUC5AC expression & immunoreactivity in primary cells of the nasal cavity. (A) Transcription analysis (RT-PCR) of MUC5AC gene in olfactory epithelial primary cells (OEPC), respiratory epithelial primary cells (REPC), tumour cell line RPMI 2650 & the concha nasalis media (c.n. media). MUC5AC transcript signal was referenced to beta-actin transcript signal. The significance was calculated by comparison of the OEPC, REPC & RPMI 2650 data with the c.n. media transcription data using an unpaired t-test. * p < 0.05; n = 4; error bars represent mean ± SD. (B) Dot blot analysis of MUC5AC protein in lysates of OEPC, REPC, RPMI 2650 & c.n. media. All OEPC & REPC cultures shown in (A,B) were cultivated for 14 days in vitro in T flasks. (C) Immunoreactivity against MUC5AC in OEPC that were first cultured for 7 days in T flask with a minimum confluency of 70% then under ALI conditions additional 20 days. Apically secreted mucus was collected at the days indicated corresponding to a mucin production of 2 to 3 days. Statistical analysis: unpaired t-test, * p < 0.05 compared to the standard model RPMI 2650. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31374872), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
MUC5AC Antibody (45M1)

Dot Blot: MUC5AC Antibody (45M1) [NBP2-15196] -

Dot Blot: MUC5AC Antibody (45M1) [NBP2-15196] - Mucin MUC5AC expression & immunoreactivity in primary cells of the nasal cavity. (A) Transcription analysis (RT-PCR) of MUC5AC gene in olfactory epithelial primary cells (OEPC), respiratory epithelial primary cells (REPC), tumour cell line RPMI 2650 & the concha nasalis media (c.n. media). MUC5AC transcript signal was referenced to beta-actin transcript signal. The significance was calculated by comparison of the OEPC, REPC & RPMI 2650 data with the c.n. media transcription data using an unpaired t-test. * p < 0.05; n = 4; error bars represent mean ± SD. (B) Dot blot analysis of MUC5AC protein in lysates of OEPC, REPC, RPMI 2650 & c.n. media. All OEPC & REPC cultures shown in (A,B) were cultivated for 14 days in vitro in T flasks. (C) Immunoreactivity against MUC5AC in OEPC that were first cultured for 7 days in T flask with a minimum confluency of 70% then under ALI conditions additional 20 days. Apically secreted mucus was collected at the days indicated corresponding to a mucin production of 2 to 3 days. Statistical analysis: unpaired t-test, * p < 0.05 compared to the standard model RPMI 2650. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31374872), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
MUC5AC Antibody (45M1)

Immunohistochemistry: MUC5AC Antibody (45M1) [NBP2-15196] -

Effect of CSLW on histopathological changes in the nasal tissues. (A) Eosinophils stain via Giemsa staining. Infiltration of the eosinophils (stained bright red); the black arrow indicates the eosinophil (scale bar = 50 μm). Immunohistochemical staining for periostin (A, middle), MUC5AC (A, lower), and 4-HNE (B). Periostin (arrows indicate allergic inflammation, scale bar = 50 μm), MUC5AC (arrows indicate mucus production, scale bar = 50 μm), and 4-HNE (arrows indicate oxidative damage, scale bar = 20 μm) are stained brown. AR, allergic rhinitis; OVA, ovalbumin; CSLW, Caesalpinia sappan Linn. heartwood water extract; DEX, dexamethasone. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36421442), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for MUC5AC Antibody (45M1)

Application
Recommended Usage

Flow Cytometry

0.5-1ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2ug/ml

Immunohistochemistry-Frozen

0.5ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Use in IHC-P reported in scientific literature (PMID:34260916).

Reviewed Applications

Read 5 reviews rated 4.2 using NBP2-15196 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: MUC5AC

Mucin 5, subtype AC (MUC5AC) is a gel-forming secreted mucin that functions as a protective barrier on surface of epithelial tissues (1-2). MUC5AC is expressed primarily by goblet cells of the surface epithelium, including in the stomach, respiratory tract, gall bladder, endocervix, and endometrium (1-3). Human MUC5AC is located on chromosome 11p15.5, consisting of 5654 amino acids and has a N-terminus, tandem-repeat region (TRR), and a C-terminus, with a theoretical molecular weight of ~585 kDa (1-4). More specifically, the N-terminus contains four von Willebrand factor type D domains, where D1 also has a leucine zipper pattern (1). The TRR is further divided into nine CysD domains and four PTS-rich tandem repeats (1). Finally, the C-terminus has a D4-, B-, C-, and CK-domain (1). The TRR is a site of heavy O-glycosylation that functions in gel and polypeptide formation (1,3).

Given that MUC5AC is the primary mucin produced and secreted by cells lining airway, it is understandable that its expression is dysregulated in a number of respiratory diseases (1,3,5,6). For instance, MUC5AC is overexpressed in asthma and the protein is also more highly glycosylated, specifically fucosylated, in the disease state (1,3,7). Additionally, its overproduction is a key feature in chronic obstructive pulmonary disease (COPD) contributing to mucosal blockage (6). Multiple pathways are associated with overproduction of MUC5AC including NFkappaB, the primary pathway for production and secretion, and also the MAPK and STAT6 pathways (3). In addition to conventional, synthetic therapeutics agents, researchers are exploring natural MUC5AC inhibitors such as flavonoids, glycosides, and steroids to treat associated disorders (3).

References

1. Krishn, S. R., Ganguly, K., Kaur, S., & Batra, S. K. (2018). Ramifications of secreted mucin MUC5AC in malignant journey: a holistic view. Carcinogenesis. https://doi.org/10.1093/carcin/bgy019

2. Ballester, B., Milara, J., & Cortijo, J. (2019). Mucins as a New Frontier in Pulmonary Fibrosis. Journal of clinical medicine. https://doi.org/10.3390/jcm8091447

3. Samsuzzaman, M., Uddin, M. S., Shah, M. A., & Mathew, B. (2019). Natural inhibitors on airway mucin: Molecular insight into the therapeutic potential targeting MUC5AC expression and production. Life sciences. https://doi.org/10.1016/j.lfs.2019.05.041

4. Uniprot (P98088)

5. Li, J., & Ye, Z. (2020). The Potential Role and Regulatory Mechanisms of MUC5AC in Chronic Obstructive Pulmonary Disease. Molecules (Basel, Switzerland). https://doi.org/10.3390/molecules25194437

6. Bonser, L. R., & Erle, D. J. (2017). Airway Mucus and Asthma: The Role of MUC5AC and MUC5B. Journal of clinical medicine. https://doi.org/10.3390/jcm6120112

Alternate Names

gastric mucin, leB, lewis B blood group antigen, major airway glycoprotein, MUC5, mucin 5, subtypes A and C, tracheobronchial/gastric, mucin 5AC, oligomeric mucus/gel-forming, mucin 5AC, oligomeric mucus/gel-forming pseudogene, mucin-5 subtype AC, tracheobronchial, mucin-5AC, TBM, tracheobronchial mucin

Gene Symbol

MUC5AC

UniProt

P98088 (Human)

Additional MUC5AC Products

Product Documents for MUC5AC Antibody (45M1)

Certificate of Analysis

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Product Specific Notices for MUC5AC Antibody (45M1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for MUC5AC Antibody (45M1)

Customer Reviews for MUC5AC Antibody (45M1) (5)

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Customer Images

Showing  1 - 5 of 5 reviews Showing All
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  • MUC5AC Antibody (45M1)
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Stomach tissue
    Species: Rat
    Verified Customer | Posted 10/20/2021
    Staining in Stomach tissue
    4% formaldehyde fixed paraffin sections
    MUC5AC Antibody (45M1) NBP2-15196
  • MUC5AC Antibody (45M1)
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Breast cancer tissue
    Species: Human
    Verified Customer | Posted 04/04/2020
    MUC5AC Antibody (45M1) NBP2-15196
  • MUC5AC Antibody (45M1)
    Name: Anonymous
    Application: Western Blot
    Sample Tested: EMT6 cells
    Species: Mouse
    Verified Customer | Posted 12/27/2019
    MUC5AC Antibody (45M1) NBP2-15196
  • MUC5AC Antibody (45M1)
    Name: Anonymous
    Application: Western Blot
    Sample Tested: EMT6 cells and CT26
    Species: Mouse
    Verified Customer | Posted 02/18/2019
    MUC5AC Antibody (45M1) NBP2-15196
  • MUC5AC Antibody (45M1)
    Name: Anonymous
    Application: Western Blot
    Sample Tested: AGS human gastric adenocarcinoma cell line and KATO-III human gastric carcinoma cell line
    Species: Human
    Verified Customer | Posted 10/17/2018
    MUC5AC Antibody (45M1) NBP2-15196

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Protocols

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FAQs for MUC5AC Antibody (45M1)

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  • Q: I would be interested in buying the anti-Mucin 5AC (clone 45M1). However, the antibodies from Abcam (your competitor), also clone "45M1" does not work in the rat. On your datasheet, you announce your antibody "cross React" with different species, including rats. Can you please confirm me?

    A:

    Our Mucin 5AC antibody NBP2-15196 has rat and other species listed as possible cross reactive species based on homology. The Abcam antibody of the same clone also lists rat as a potential species. Unlike Abcam, we will apply our guarantee to species that have 90% homology or higher.

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