Musashi-1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-32812

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Predicted:

Bovine (100%), Porcine (98%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Western Blot, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Musashi-1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Musashi-1. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (81%).

Localization

Cytoplasm, Nucleus

Marker

Neuronal Stem Cell Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

39 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Musashi-1 Antibody - BSA Free

Western Blot: Musashi-1 Antibody [NBP1-32812]

Western Blot: Musashi-1 Antibody [NBP1-32812]

Western Blot: Musashi-1 Antibody [NBP1-32812] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Musashi 1 antibody [N3C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunohistochemistry-Frozen: Musashi-1 Antibody [NBP1-32812]

Immunohistochemistry-Frozen: Musashi-1 Antibody [NBP1-32812]

Immunohistochemistry-Frozen: Musashi-1 Antibody [NBP1-32812] - Frozen sectioned adult mouse retina. Green: Musashi 1 protein stained by Musashi 1 antibody [N3C3] diluted at 1:250. Red: Protein kinase C alpha staining. Blue: Fluoroshield with DAPI.
Western Blot: Musashi-1 Antibody [NBP1-32812]

Western Blot: Musashi-1 Antibody [NBP1-32812]

Western Blot: Musashi-1 Antibody [NBP1-32812] - Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Musashi 1 antibody [N3C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: Musashi-1 Antibody [NBP1-32812]

Immunocytochemistry/ Immunofluorescence: Musashi-1 Antibody [NBP1-32812]

Immunocytochemistry/Immunofluorescence: Musashi-1 Antibody [NBP1-32812] - Paraformaldehyde-fixed A549, using antibody at 1:200 dilution.
Immunohistochemistry-Frozen: Musashi-1 Antibody [NBP1-32812]

Immunohistochemistry-Frozen: Musashi-1 Antibody [NBP1-32812]

Immunohistochemistry-Frozen: Musashi-1 Antibody [NBP1-32812] - MSI1 antibody [N3C3] detects MSI1 protein on adult mouse brain by immunohistochemical analysis. Sample: Frozen section of adult mouse brain. MSI1 antibody [N3C3] diluted at 1:500.
Musashi-1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Musashi-1 Antibody [NBP1-32812] -

MSI1 antibody [N3C3] detects MSI1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: MSI1 protein stained by MSI1 antibody [N3C3] (NBP1-32812) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.

Applications for Musashi-1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Frozen

1:100-1:1000

Immunoprecipitation

Assay dependent

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Musashi-1

Musashi1, a neural RNA-binding protein, plays an important role in regulating cell differentiation in precursor cells. Recently, expression of Musashi1 has been detected in human tumor tissues such as gliomas and melanomas, suggesting its involvement in oncogenic development. Results suggest that Musashi1 expression may be an important factor in the development of several types of carcinoma such as human hepatoma, and may be a useful molecular marker for tumor detection. (1). Recent findings demonstrate that translational regulation contributes to cell-fate specification. The evolutionarily conserved, neural RNA-binding protein Musashi1 controls neural cell fate. The protein functions in maintenance of the stem-cell state, differentiation, and tumorigenesis by repressing translation of particular mRNAs. In mammals it might play an important role in activating Notch signaling by repressing translation of the Notch inhibitor m-Numb (2).

Alternate Names

MSI1, Musashi1

Gene Symbol

MSI1

Additional Musashi-1 Products

Product Documents for Musashi-1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Musashi-1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Citations for Musashi-1 Antibody - BSA Free

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Protocols

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FAQs

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