MVP Antibody (2H3-1A6) - Azide and BSA Free

Novus Biologicals | Catalog # H00009961-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 2H3-1A6

Format

Azide and BSA Free
View all MVP Primary Antibodies »

Product Specifications

Immunogen

MVP (AAH15623, 1 a.a. ~ 893 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MATEEFIIRIPPYHYIHVLDQNSNVSRVEVGPKTYIRQDNERVLFAPMRMVTVPPRHYCTVANPVSRDAQGLVLFDVTGQVRLRHADLEIRLAQDPFPLYPGEVLEKDITPLQVVLPNTALHLKALLDFEDKDGDKVVAGDEWLFEGPGTYIPRKEVEVVEIIQATIIRQNQALRLRARKECWDRDGKERVTGEEWLVTTVGAYLPAVFEEVLDLVDAVILTEKTALHLRARRNFRDFRGVSRRTGEEWLVTVQDTEAHVPDVHEEVLGVVPITTLGPHNYCVILDPVGPDGKNQLGQKRVVKGEKSFFLQPGEQLEQGIQDVYVLSEQQGLLLRALQPLEEGEDEEKVSHQAGDHWLIRGPLEYVPSAKVEVVEERQAIPLDENEGIYVQDVKTGKVRAVIGSTYMLTQDEVLWEKELPPGVEELLNKGQDPLADRGEKDTAKSLQPLAPRNKTRVVSYRVPHNAAVQVYDYREKRARVVFGPELVSLGPEEQFTVLSLSAGRPKRPHARRALCLLLGPDFFTDVITIETADHARLQLQLAYNWHFEVNDRKDPQETAKLFSVPDFVGDACKAIASRVRGAVASVTFDDFHKNSARIIRTAVFGFETSEAKGPDGMALPRPRDQAVFPQNGLVVSSVDVQSVEPVDQRTRDALQRSVQLAIEITTNSQEAAAKHEAQRLEQEARGRLERQKILDQSEAEKARKELLELEALSMAVESTGTAKAEAESRAEAARIEGEGSVLQAKLKAQALAIETEAELQRVQKVRELELVYARAQLELEVSKAQQLAEVEVKKFKQMTEAIGPSTIRDLAVAGPEMQVKLLQSLGLKSTLITDGSTPINLFNTAFGLLGMGPEGQPLGRRVASGPSPGEGISPQSAQAPQAPGDNHVVPVLR

Localization

Cytoplasmic, 5% are nucleus associated and localize to the nuclear pore complexes

Specificity

MVP - major vault protein

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for MVP Antibody (2H3-1A6) - Azide and BSA Free

Western Blot: MVP Antibody (2H3-1A6) [H00009961-M01]

Western Blot: MVP Antibody (2H3-1A6) [H00009961-M01]

MVP-Antibody-2H3-1A6-Western-Blot-H00009961-M01-img0011.jpg
Immunocytochemistry/ Immunofluorescence: MVP Antibody (2H3-1A6) [H00009961-M01]

Immunocytochemistry/ Immunofluorescence: MVP Antibody (2H3-1A6) [H00009961-M01]

Immunocytochemistry/Immunofluorescence: MVP Antibody (2H3-1A6) [H00009961-M01] - Analysis of monoclonal antibody to MVP on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: MVP Antibody (2H3-1A6) [H00009961-M01]

Immunohistochemistry-Paraffin: MVP Antibody (2H3-1A6) [H00009961-M01]

Immunohistochemistry-Paraffin: MVP Antibody (2H3-1A6) [H00009961-M01] - Analysis of monoclonal antibody to MVP on formalin-fixed paraffin-embedded human lung, adenosquamous cell carcinoma. Antibody concentration 3 ug/ml.
Western Blot: MVP Antibody (2H3-1A6) [H00009961-M01]

Western Blot: MVP Antibody (2H3-1A6) [H00009961-M01]

Western Blot: MVP Antibody (2H3-1A6) [H00009961-M01] - MVP monoclonal antibody (M01), clone 2H3-1A6 Analysis of MVP expression in Hela S3 NE.
ELISA: MVP Antibody (2H3-1A6) [H00009961-M01]

ELISA: MVP Antibody (2H3-1A6) [H00009961-M01]

ELISA: MVP Antibody (2H3-1A6) [H00009961-M01] - Detection limit for recombinant GST tagged MVP is approximately 1ng/ml as a capture antibody.
MVP Antibody (2H3-1A6)

Western Blot: MVP Antibody (2H3-1A6) [H00009961-M01] -

Western Blot: MVP Antibody (2H3-1A6) [H00009961-M01] - Immunoblot validation & characterization of dysferlin.A) Western blot of several proteins that were increased or decreased in DBA patients including dysferlin (DYSF), gamma-adducin (ADD3), major vault protein (MVP), serine palmitoyltransferase 1 & 2 (SPTLC1 & SPTLC2), HLA-ABC, & intercellular adhesion molecule 4 (ICAM4) with beta -actin (ACTB) used as a loading control. Six individual control samples that were included in the pooled controls for proteomic analysis were used for comparison to individual DBA patient samples. B) Confocal images of intact RBCs from DBA patient D1 & healthy control C2 showing localization of dysferlin (green) at the membrane where spectrin (red) is localized. Dysferlin fluorescence signal in the membrane of DBA RBCs is present in punctuate patterns & is evident above the autofluorescent background seen in the cytoplasm as highlighted in the zoomed images on the right panel. C) Western blot showing the enrichment of dysferlin in the RBC ghosts (WG) & the inside-out vesicles (IOV) but not in the RBC membrane cytoskeletal extract (CE) of DBA patients D1 & D2. D) Distribution of dysferlin spectral counts versus gel slice number for DBA samples D1 & D2. Higher abundance of dysferlin in D1 with high molecular weight dysferlin crosslinks & low mass protein fragments correlate with immunoblots shown in panel A. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/24454878), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for MVP Antibody (2H3-1A6) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence, immunohistochemistry (paraffin), and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: MVP

This gene encodes the major vault protein which is a lung resistance-related protein. Vaults are multi-subunit structures that may be involved in nucleo-cytoplasmic transport. This protein mediates drug resistance, perhaps via a transport process. It is widely distributed in normal tissues, and overexpressed in multidrug-resistant cancer cells. The protein overexpression is a potentially useful marker of clinical drug resistance. This gene produces two transcripts by using two alternative exon 2 sequences; however, the open reading frames are the same in both transcripts.

Alternate Names

LRPVAULT1, Lung resistance-related protein, major vault protein

Entrez Gene IDs

9961 (Human)

Gene Symbol

MVP

OMIM

605088 (Human)

UniProt

AAH15623 (Human)

Additional MVP Products

Product Documents for MVP Antibody (2H3-1A6) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for MVP Antibody (2H3-1A6) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for MVP Antibody (2H3-1A6) - Azide and BSA Free

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Protocols

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FAQs for MVP Antibody (2H3-1A6) - Azide and BSA Free

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  • Q: Are there any MVP antibodies that will recognize mouse and can be used for immunofluorescence?

    A: At this time we do not have an MVP antibody that will recognize mouse and has been used for immunofluorescence. If you would like to try an antibody in an untested species or application you would qualify for our Innovator's Reward program. Through this program if you complete an online review with image, detailing your positive or negative results we will send you a discount voucher for 100% of the purchase price of the reviewed product.

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