Mycoplasma Detection

A highly sensitive colorimetric microplate-based assay designed to detect the eight mycoplasma species known to cause 95% of eukaryotic cell culture contamination.

R&D Systems MycoProbe™ Mycoplasma Detection Kit (Catalog # CUL001B) permits high-throughput, routine screening of cell cultures for mycoplasma contamination. Mycoplasma contamination is one of the most common and serious problems when culturing eukaryotic cells. Mycoplasma contamination has the potential to alter the phenotypic characteristics of the cells and can negatively impact results. Since mycoplasma is typically not visible and does not respond to antibiotics, an alternative, sensitive, and reliable detection method is required.

MycoProbe Mycoplasma Detection Kit (Catalog # CUL001B)

Assay Features

  • High-throughput screening (microplate-based) assay
  • No false positives from amplicon contamination
  • Highly sensitive (comparable to PCR)
  • Culturing in antibiotic-free media is not required
  • Pellet or supernate sample may be used from fresh or frozen cells
  • Rapid results in 4.5 hours
  • Reagents sufficient for 96 tests

Kit Contents

Each MycoProbe Mycoplasma Detection Kit contains all of the necessary components for a ready-to-run assay and requires no additional development. Each kit contains sufficient reagents for 96 tests. We recommend running samples in duplicate with positive and negative controls.

  • Cell Lysis Buffer
  • Hybridization Plate
  • Streptavidin Capture Plate
  • Diluents
  • Substrate
  • Conjugate
  • Pan-Specific Oligonucleotide Probes
  • Synthetic DNA Oligonucleotide Positive Control

Mycoplasma Bacteria Detected:

  • M. hyorhinis
  • M. arginini
  • M. fermentans
  • M. orale
  • M. pirum
  • M. hominis
  • M. salivarium
  • A. laidlawii