MyoD Antibody (5.2F) - BSA Free

Novus Biologicals | Catalog # NBP3-30815

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Porcine, Chicken

Applications

Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 5.2F

Format

BSA Free
View all MyoD Primary Antibodies »

Product Specifications

Immunogen

Recombinant mouse MyoD protein.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for MyoD Antibody (5.2F) - BSA Free

MyoD Antibody (5.2F)

Immunocytochemistry/ Immunofluorescence: MyoD Antibody (5.2F) [NBP3-30815] -

Immunocytochemistry/ Immunofluorescence: MyoD Antibody (5.2F) [NBP3-30815] - Figure 4. MyoD immunostaining of nuclei in MyoD transfected cells induced to undergo muscle cell differentiation.
MyoD Antibody (5.2F)

Immunocytochemistry/ Immunofluorescence: MyoD Antibody (5.2F) [NBP3-30815] -

Immunocytochemistry/ Immunofluorescence: MyoD Antibody (5.2F) [NBP3-30815] - Figure 3. MyoD immunostaining of nuclei in MyoD transfected cells induced to undergo muscle cell differentiation.
MyoD Antibody (5.2F)

Immunocytochemistry/ Immunofluorescence: MyoD Antibody (5.2F) [NBP3-30815] -

Immunocytochemistry/ Immunofluorescence: MyoD Antibody (5.2F) [NBP3-30815] - Figure 2. MyoD immunostaining of nuclei in MyoD transfected cells induced to undergo muscle cell differentiation.
MyoD Antibody (5.2F)

Western Blot: MyoD Antibody (5.2F) [NBP3-30815] -

Western Blot: MyoD Antibody (5.2F) [NBP3-30815] - Left: Immunohistochemical staining using MyoD antibody on formalin fixed, paraffin embedded human rhabdomyosarcoma. Right: Western blot using 60 ng of purified MyoD protein with MyoD antibody at 2 ug/ml and detected using goat anti-mouse HRP and visualized with Pierce West-Femto substrate.

Applications for MyoD Antibody (5.2F) - BSA Free

Application
Recommended Usage

Immunohistochemistry-Frozen

: 2-4 ug/ml

Immunohistochemistry-Paraffin

: 2-4 ug/ml

Immunoprecipitation

: 2 ug/mg of protein lysate

Western Blot

: 1 ug/ml
Application Notes
.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

Phosphate buffered saline

Format

BSA Free

Preservative

0.08% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MyoD

The Myogenic determination gene (MyoD) was first identified by the virtue of its ability to convert embryonic mouse fibroblast cells to muscle cells. It was subsequently shown that forced expression of MyoD (human homolog is myf 3) gene in a wide variety of normal and neoplastic cells could either convert the cells to muscle cells or activate a set of the otherwise transcriptionally inactive muscle-specific genes in these cells. The regulatory domain of the MyoD gene product lies within a 70 amino acid region and comprises a basic DNA binding motif and a helix-loop-helix (HLH) dimerization motif. Subsequent studies identified three other genes whose products shared sequence homology for the basic HLH domain of MyoD. These are; myf5, myogenin (human homolog is myf4) and myf6 (also known as MRF4 and herculin).

Long Name

Myoblast Determination Protein 1

Alternate Names

bHLHc1, MYF3, MYOD1, PUM

Gene Symbol

MYOD1

Additional MyoD Products

Product Documents for MyoD Antibody (5.2F) - BSA Free

Certificate of Analysis

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Product Specific Notices for MyoD Antibody (5.2F) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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