MyoD Antibody (5.8A) [DyLight 488]

Novus Biologicals | Catalog # NB100-56511G

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Porcine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Knockdown Validated

Label

DyLight 488 (Excitation = 493 nm, Emission = 518 nm)

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 5.8A
View all MyoD Primary Antibodies »

Product Specifications

Immunogen

The 5.8A antibody was made against recombinant mouse MyoD protein but it also recognizes human (myf3), rat, and cat homologs. The epitope of this antibody was mapped to a region within aa 180-189 of mouse MyoD (NP_002469).

Specificity

In Rh-30, a ~45 kDa band should be observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Scientific Data Images for MyoD Antibody (5.8A) [DyLight 488]

Immunocytochemistry/Immunofluorescence: MyoD Antibody (5.8A) [DyLight 488] [NB100-56511G] - Cell Lines Tested: mouse skeletal muscle-derived primary cell populationTest Sample Preparation: mouse skeletal muscle digested by collagenase type 2System: Super Sensitive High Resolution Confocal Laser Microscope (LSM880 with Airyscan)Excitation Wavelength: 488nmEmission Filter: 562nmImage is courtesy of customer review.

Applications for MyoD Antibody (5.8A) [DyLight 488]

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Knockout Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using NB100-56511G in the following applications:

Spectra Viewer

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: MyoD

The Myogenic determination gene (MyoD) was first identified by the virtue of its ability to convert embryonic mouse fibroblast cells to muscle cells. It was subsequently shown that forced expression of MyoD (human homolog is myf 3) gene in a wide variety of normal and neoplastic cells could either convert the cells to muscle cells or activate a set of the otherwise transcriptionally inactive muscle-specific genes in these cells. The regulatory domain of the MyoD gene product lies within a 70 amino acid region and comprises a basic DNA binding motif and a helix-loop-helix (HLH) dimerization motif. Subsequent studies identified three other genes whose products shared sequence homology for the basic HLH domain of MyoD. These are; myf5, myogenin (human homolog is myf4) and myf6 (also known as MRF4 and herculin).

Long Name

Myoblast Determination Protein 1

Alternate Names

bHLHc1, MYF3, MYOD1, PUM

Gene Symbol

MYOD1

Additional MyoD Products

Product Documents for MyoD Antibody (5.8A) [DyLight 488]

Certificate of Analysis

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Product Specific Notices for MyoD Antibody (5.8A) [DyLight 488]



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for MyoD Antibody (5.8A) [DyLight 488] (1)

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  • MyoD Antibody (5.8A) [DyLight 488]
    Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: Mouse skeletal muscle
    Species: Mouse
    Verified Customer | Posted 04/18/2017
    Sample Information Cell Lines Tested: mouse skeletal muscle-derived primary cell population Species: mouse Test Sample Preparation: mouse skeletal muscle digested by collagenase type 2 Fixation Solution and Conditions: 4% formaldehyde treatment for 20min at RT Blocking Solution: no-treatment because of using fluorescence conjugated antibody in this experiment Time and Temperature: - Washing Wash Buffer: PBS Time: 5min Repetitions: 2 times Primary Antibody Diluent: 0.5% tritonX-100 in PBS Dilutions Tested: 1:50 Time and Temperature: overnight at 4℃ Washing Wash Buffer: PBS Time: 5min Repetitions: 2 times Detection System: Super Sensitive High Resolution Confocal Laser Microscope (LSM880 with Airyscan) Excitation Wavelength: 488nm Emission Filter: 562nm
    MyoD Antibody (5.8A) [DyLight 488] NB100-56511G

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