Myosin Light Chain 2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-85541

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Myosin Light Chain 2 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: GPINFTVFLTMFGEKLKGADPEETILNAFKVFDPEGKGVLKADYVREMLTTQAERFSKEEVDQMFAAFPPDVTGNLDYKNLVHIITHGE

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 25299188). Rat (82%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Myosin Light Chain 2 Antibody - BSA Free

Immunohistochemistry-Paraffin: Myosin Light Chain 2 Antibody [NBP1-85541]

Immunohistochemistry-Paraffin: Myosin Light Chain 2 Antibody [NBP1-85541]

Immunohistochemistry-Paraffin: Myosin Light Chain 2 Antibody [NBP1-85541] - Analysis in human heart muscle and pancreas tissues. Corresponding Myosin Light Chain 2 RNA-seq data are presented for the same tissues.
Western Blot: Myosin Light Chain 2 Antibody [NBP1-85541]

Western Blot: Myosin Light Chain 2 Antibody [NBP1-85541]

Western Blot: Myosin Light Chain 2 Antibody [NBP1-85541] - Analysis in human heart tissue.
Immunohistochemistry-Frozen: Myosin Light Chain 2 Antibody [NBP1-85541]

Immunohistochemistry-Frozen: Myosin Light Chain 2 Antibody [NBP1-85541]

Immunohistochemistry-Frozen: Myosin Light Chain 2 Antibody [NBP1-85541] - Mlc2v staining on E11.5 mouse Right Ventricle. Fixed 4% PFA overnight. Blocked with 1% BSA. Primary antibody at 1ug/mL. Secondary antibody at 1:1000, conjugated to Alexa Fluor 488. Image submitted by a verified customer review.
Immunohistochemistry-Paraffin: Myosin Light Chain 2 Antibody [NBP1-85541]

Immunohistochemistry-Paraffin: Myosin Light Chain 2 Antibody [NBP1-85541]

Immunohistochemistry-Paraffin: Myosin Light Chain 2 Antibody [NBP1-85541] - Staining of human heart muscle shows moderate to strong cytoplasmic positivity in cardiomyocytes.
Immunohistochemistry-Paraffin: Myosin Light Chain 2 Antibody [NBP1-85541]

Immunohistochemistry-Paraffin: Myosin Light Chain 2 Antibody [NBP1-85541]

Immunohistochemistry-Paraffin: Myosin Light Chain 2 Antibody [NBP1-85541] - Staining of human liver shows no positivity in hepatocytes as expected.
Immunohistochemistry: Myosin Light Chain 2 Antibody [NBP1-85541]

Immunohistochemistry: Myosin Light Chain 2 Antibody [NBP1-85541]

Immunohistochemistry: Myosin Light Chain 2 Antibody [NBP1-85541] - Immunohistochemical staining of human skeletal muscle shows moderate cytoplasmic positivity in myocytes.
Immunohistochemistry: Myosin Light Chain 2 Antibody [NBP1-85541]

Immunohistochemistry: Myosin Light Chain 2 Antibody [NBP1-85541]

Immunohistochemistry: Myosin Light Chain 2 Antibody [NBP1-85541] - Immunohistochemical staining of human pancreas shows no positivity in exocrine glandular cells as expected.

Applications for Myosin Light Chain 2 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:2500 - 1:5000

Immunohistochemistry-Frozen

Validated from a verified customer review

Immunohistochemistry-Paraffin

1:2500 - 1:5000

Western Blot

0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Reviewed Applications

Read 1 review rated 5 using NBP1-85541 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Myosin Light Chain 2

MYL2 (myosin, light chain 2, regulatory, cardiac, slow), also known as MLC-2, MLC2v. Entrez protein NP_000423. MYL2 associated with cardiac myosin beta (or slow) heavy chain. Ca+ triggers the phosphorylation of regulatory light chain that in turn triggers contraction. It is an hexamer of two heavy chains and four light chains that is predominantly expressed in adult cardiac ventricle muscle. Mutations in MYL2 are associated with mid-left ventricular chamber type hypertrophic cardiomyopathy.

Alternate Names

cardiac ventricular myosin light chain 2, CMH10myosin light chain 2, DKFZp779C0562, MLC2, MLC-2, MLC-2v, myosin regulatory light chain 2, ventricular/cardiac muscle isoform, myosin, light chain 2, regulatory, cardiac, slow, myosin, light polypeptide 2, regulatory, cardiac, slow, regulatory light chain of myosin, RLC of myosin, slow cardiac myosin regulatory light chain 2

Gene Symbol

MYL2

Additional Myosin Light Chain 2 Products

Product Documents for Myosin Light Chain 2 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Myosin Light Chain 2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Myosin Light Chain 2 Antibody - BSA Free

Customer Reviews for Myosin Light Chain 2 Antibody - BSA Free (1)

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  • Myosin Light Chain 2 Antibody
    Name: Anonymous
    Application: Immunohistochemistry-Frozen
    Sample Tested: E11.5 mouse embryo fixed in 4% PFA
    Species: Mouse
    Verified Customer | Posted 01/07/2021
    Mlc2v staining on E11.5 mouse Right Ventricle
    Fixed 4% PFA overnight. Blocked with 1% BSA Primary antibody concentration - 1ug/mL Secondary antibody - Invitrogen Alexa Fluor 488 Secondary antibody - 1:1000
    Myosin Light Chain 2 Antibody - BSA Free NBP1-85541

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