Myosin VIIb Antibody - BSA Free
Novus Biologicals | Catalog # NBP3-27882
Key Product Details
Species Reactivity
Human, Mouse
Applications
Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit
Format
BSA Free
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Product Specifications
Immunogen
Myosin VIIb antibody was raised against a peptide corresponding to 15 amino acids near the center of human Myosin VIIb. The immunogen is located within amino acids 1100-1150 of Myosin VIIb.
Clonality
Polyclonal
Host
Rabbit
Scientific Data Images for Myosin VIIb Antibody - BSA Free
Immunohistochemistry: Myosin VIIb Antibody [NBP3-27882] -
Immunohistochemistry: Myosin VIIb Antibody [NBP3-27882] - Figure 3 Immunohistochemistry Validation of Myosin VIIb in Human Kidney TissueImmunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Myosin VIIb antibody at 5 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Western Blot: Myosin VIIb Antibody [NBP3-27882] -
Western Blot: Myosin VIIb Antibody [NBP3-27882] - Figure 2 Western Blot Validation in Human and Mouse Cell LinesLoading: 15 u of lysates per lane.Antibodies: Myosin VIIb (2 u/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot: Myosin VIIb Antibody [NBP3-27882] -
Western Blot: Myosin VIIb Antibody [NBP3-27882] - Figure 2 Western Blot Validation in Human and Mouse Cell LinesLoading: 15 u of lysates per lane.Antibodies: Myosin VIIb (2 u/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Immunocytochemistry/ Immunofluorescence: Myosin VIIb Antibody [NBP3-27882] -
Immunocytochemistry/ Immunofluorescence: Myosin VIIb Antibody [NBP3-27882] - Figure 4 Immunofluorescence Validation of Myosin VIIb in Human Kidney TissueImmunofluorescent analysis of 4% paraformaldehyde-fixed human kidney tissue labeling Myosin VIIb with at 20 u/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
Immunocytochemistry/ Immunofluorescence: Myosin VIIb Antibody [NBP3-27882] -
Immunocytochemistry/ Immunofluorescence: Myosin VIIb Antibody [NBP3-27882] - Figure 4 Immunofluorescence Validation of Myosin VIIb in Human Kidney TissueImmunofluorescent analysis of 4% paraformaldehyde-fixed human kidney tissue labeling Myosin VIIb with at 20 u/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
Immunohistochemistry: Myosin VIIb Antibody [NBP3-27882] -
Immunohistochemistry: Myosin VIIb Antibody [NBP3-27882] - Figure 3 Immunohistochemistry Validation of Myosin VIIb in Human Kidney Tissue Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Myosin VIIb antibody at 5 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Western Blot: Myosin VIIb Antibody [NBP3-27882] -
Western Blot: Myosin VIIb Antibody [NBP3-27882] - Figure 1 Western Blot Validation in HeLa Cell Lysate with (A) the Absence and (B) the Presence of Blocking PeptideLoading: 15 u of lysates per lane.Antibodies: Myosin VIIb (1 u/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot: Myosin VIIb Antibody [NBP3-27882] -
Western Blot: Myosin VIIb Antibody [NBP3-27882] - Figure 1 Western Blot Validation in HeLa Cell Lysate with (A) the Absence and (B) the Presence of Blocking PeptideLoading: 15 u of lysates per lane.Antibodies: Myosin VIIb (1 u/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Applications for Myosin VIIb Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry-Paraffin
5 ug/mL
Western Blot
1 - 2 ug/mL
Formulation, Preparation, and Storage
Purification
Peptide affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Myosin VIIb
Additional Myosin VIIb Products
Product Documents for Myosin VIIb Antibody - BSA Free
Certificate of Analysis
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Product Specific Notices for Myosin VIIb Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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