NAA10 Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-38359

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all NAA10 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 161-235 of human NAA10 (NP_003482.1).

Sequence:
HLELKEKGRHVVLGAIENKVESKGNSPPSSGEACREEKGLAAEDSGGDSKDLSEVSETTESTDVKDSSEASDSAS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

26 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NAA10 Antibody - BSA Free

NAA10 Antibody

Immunohistochemistry: NAA10 Antibody [NBP3-38359] -

Immunohistochemistry: NAA10 Antibody [NBP3-38359] - Immunohistochemistry analysis of paraffin-embedded Mouse lung using NAA10 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
NAA10 Antibody

Western Blot: NAA10 Antibody [NBP3-38359] -

Western Blot: NAA10 Antibody [NBP3-38359] - Western blot analysis of various lysates using NAA10 Rabbit pAb at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
NAA10 Antibody

Immunohistochemistry: NAA10 Antibody [NBP3-38359] -

Immunohistochemistry: NAA10 Antibody [NBP3-38359] - Immunohistochemistry analysis of paraffin-embedded Human stomach using NAA10 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
NAA10 Antibody

Immunocytochemistry/ Immunofluorescence: NAA10 Antibody [NBP3-38359] -

Immunocytochemistry/ Immunofluorescence: NAA10 Antibody [NBP3-38359] - Immunofluorescence analysis of U2OS cells using NAA10 Rabbit pAb. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
NAA10 Antibody

Immunohistochemistry: NAA10 Antibody [NBP3-38359] -

Immunohistochemistry: NAA10 Antibody [NBP3-38359] - Immunohistochemistry analysis of paraffin-embedded Rat lung using NAA10 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
NAA10 Antibody

Immunohistochemistry: NAA10 Antibody [NBP3-38359] -

Immunohistochemistry: NAA10 Antibody [NBP3-38359] - Immunohistochemistry analysis of paraffin-embedded Human breast cancer using NAA10 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Applications for NAA10 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:100

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: NAA10

N-alpha-acetylation is one of the most common protein modifications that occurs during protein synthesis and involves the transfer of an acetyl group from acetyl-coenzyme A to the protein alpha-amino group. ARD1A, together with NATH (NARG1; MIM 608000), is part of a major N-alpha-acetyltransferase complex responsible for alpha-acetylation of proteins and peptides (Sanchez-Puig and Fersht, 2006 [PubMed 16823041]).[supplied by OMIM]

Alternate Names

ARD1, ARD1 homolog A, N-acetyltransferase (S. cerevisiae), ARD1 homolog, N-acetyltransferase (S. cerevisiae), ARD1AARD1 homolog, N-acetyltransferase, EC 2.3.1.-, EC 2.3.1.88, MGC71248, N(alpha)-acetyltransferase 10, NatA catalytic subunit, N-acetyltransferase, N-acetyltransferase ARD1, human homolog of, N-alpha-acetyltransferase 10, NatA catalytic subunit, N-terminal acetyltransferase complex ARD1 subunit homolog A

Gene Symbol

NAA10

Additional NAA10 Products

Product Documents for NAA10 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for NAA10 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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