NBR1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-71703

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Sandwich ELISA Capture, Immunocytochemistry/ Immunofluorescence, Simple Western

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all NBR1 Primary Antibodies »

Product Specifications

Immunogen

A genomic peptide made to an internal region of the human NBR1 protein (within residues 50-250). [Swiss-Prot Q14596]

Reactivity Notes

Immunogen has 75% identity to the rat protein.

Localization

Cytoplasm. Cytoplasmic vesicle - autophagosome. Lysosome. Cytoplasm - myofibril - sarcomere - M line. Note: In cardiac muscles localizes to the sarcomeric M line. Is targeted to lysosomes for degradation.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NBR1 Antibody - BSA Free

Western Blot: NBR1 AntibodyBSA Free [NBP1-71703]

Western Blot: NBR1 AntibodyBSA Free [NBP1-71703]

Western Blot: NBR1 Antibody [NBP1-71703] - The antibody detects HeLa cell NBR1 (about 130 kDa). It detects a 100 kDa band in mouse brain. Western blot image submitted by a verified customer review.
Immunocytochemistry/ Immunofluorescence: NBR1 Antibody - BSA Free [NBP1-71703]

Immunocytochemistry/ Immunofluorescence: NBR1 Antibody - BSA Free [NBP1-71703]

Immunocytochemistry/Immunofluorescence: NBR1 Antibody [NBP1-71703] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with NBR1 Antibody (NBP1-71703) at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.
Immunohistochemistry: NBR1 Antibody - BSA Free [NBP1-71703]

Immunohistochemistry: NBR1 Antibody - BSA Free [NBP1-71703]

Immunohistochemistry: NBR1 Antibody [NBP1-71703] - Staining of NBRI in mouse prostate.
Western Blot: NBR1 AntibodyBSA Free [NBP1-71703]

Western Blot: NBR1 AntibodyBSA Free [NBP1-71703]

Western Blot: NBR1 Antibody [NBP1-71703] - WB analysis of NBR1 in HepG2 cell lysates.
Western Blot: NBR1 AntibodyBSA Free [NBP1-71703]

Western Blot: NBR1 AntibodyBSA Free [NBP1-71703]

Western Blot: NBR1 Antibody [NBP1-71703] - WB analysis of 30 ug whole cell lysate of HeLa cells using NBR1 antibody.
Simple Western: NBR1 AntibodyBSA Free [NBP1-71703]

Simple Western: NBR1 AntibodyBSA Free [NBP1-71703]

Simple Western: NBR1 Antibody [NBP1-71703] - Simple Western lane view shows a specific band for NBR1 in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Applications for NBR1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:100

Immunohistochemistry-Paraffin

1:100

Sandwich ELISA Capture

reported by customer review

Simple Western

1:400

Western Blot

1 - 2 ug/ml
Application Notes
Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in HepG2 lysate 0.5 mg/mL, separated by Size, antibody dilution of 1:400, apparent MW was 73 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.

Reviewed Applications

Read 3 reviews rated 3.7 using NBP1-71703 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.24 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NBR1

NBR1 (Next to BRCA1 gene 1) is a protein which is ubiquitously expressed and highly conserved in eukaryotic organisms. NBR1 is implicated in TITIN kinase signaling in muscles and mitigates FGFR signaling through NBR1- SPRED2 interactions. Animals expressing truncated NBR1 lacking the C-terminal region has been shown to develop excessive bone mass due to increased activity of osteoblasts, suggesting NBR1's role in modulating bone metabolism in vivo. NBR1 functions as a negative regulator of p38 MAPK activity and has also been discovered as a binding partner of ATG8 family members including LC3, GABARAP, GABARAP-like 1/GABARAPL1, and GATE-16/GABARAPL2 in mammalian cells wherein these proteins are known to play essential role in formation of autophagosomes. Moreover, NBR1 interacts with ubiquitin/ubiquitinated proteins via ubiquitin-associated (UBA) domain and also interacts with TRIM55, SQSTM1, RNF29, USP8 etc. Based on these properties, it has been proposed that NBR1 functions as a cargo adapter for autophagic degradation of ubiquitinated substrates in a similar way to p62.

Alternate Names

Cell migration-inducing gene 19 protein, FLJ98272, KIAA0049CA125, M17S2FLJ55359,1A1-3B, Membrane component chromosome 17 surface marker 2,1A13B, membrane component, chromosome 17, surface marker 2 (ovarian carcinoma antigenCA125), migration-inducing protein 19, neighbor of BRCA1 gene 1, Neighbor of BRCA1 gene 1 protein, next to BRCA1 gene 1 protein, Protein 1A1-3B

Entrez Gene IDs

4077 (Human); 17966 (Mouse)

Gene Symbol

NBR1

UniProt

Q14596 (Human), P97432 (Mouse)

Additional NBR1 Products

Product Documents for NBR1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for NBR1 Antibody - BSA Free

Manufactured by Genomic Antibody Technology™. GAT FAQs

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for NBR1 Antibody - BSA Free

Customer Reviews for NBR1 Antibody - BSA Free (3)

3.7 out of 5
3 Customer Ratings
5 Stars
0%
4 Stars
67%
3 Stars
33%
2 Stars
0%
1 Stars
0%

Have you used NBR1 Antibody - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 3 of 3 reviews Showing All
Filter By:
Clear Filters X
  • Antibody works
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Liver tissue
    Species: Mouse
    Verified Customer | Posted 12/10/2024
    WB_NBR1-Liver tissues
    The antibody was diluted 1:1000 in 1X TBST. Multiple bands were observed high molecular weight regions as well.
    NBR1 Antibody - BSA Free NBP1-71703
  • NBR1 Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: THP-1 macrophage lysate
    Species: Human
    Verified Customer | Posted 12/05/2019
    This antibody was used to capture NBR1 from THP-1 macrophage lysate, and detected using a mouse anti-NBR1 antibody. A titration of the lysate is shown here.
    NBR1 Antibody - BSA Free NBP1-71703
  • NBR1 Antibody
    Name: Liu Yue
    Application: Western Blot
    Sample Tested: hela cell and Mouse brain
    Species: Human and Mouse
    Verified Customer | Posted 07/14/2017
    The NBP1-71703 could detect Hela cell NBR1,a band about 130KDa. It detects a 100KDa band in mouse brain.
    NBR1 Antibody - BSA Free NBP1-71703

There are no reviews that match your criteria.

Showing  1 - 3 of 3 reviews Showing All

Protocols

View specific protocols for NBR1 Antibody - BSA Free (NBP1-71703):


Immunocytochemistry Protocol

Culture cells to appropriate density in 35 mm culture dishes or 6-well plates.

1. Remove culture medium and add 10% formalin to the dish. Fix at room temperature for 30 minutes.
2. Remove the formalin and add ice cold methanol. Incubate for 5-10 minutes.
3. Remove methanol and add washing solution (i.e. PBS). Be sure to not let the specimen dry out. Wash three times for 10 minutes.
4. To block nonspecific antibody binding incubate in 10% normal goat serum from 1 hour to overnight at room temperature.
5. Add primary antibody at appropriate dilution and incubate at room temperature from 2 hours to overnight at room temperature.
6. Remove primary antibody and replace with washing solution. Wash three times for 10 minutes.
7. Add secondary antibody at appropriate dilution. Incubate for 1 hour at room temperature.
8. Remove antibody and replace with wash solution, then wash for 10 minutes. Add Hoechst 33258 to wash solution at 1:25,0000 and incubate for 10 minutes. Wash a third time for 10 minutes.
9. Cells can be viewed directly after washing. The plates can also be stored in PBS containing Azide covered in Parafilm (TM). Cells can also be cover-slipped using Fluoromount, with appropriate sealing.

*The above information is only intended as a guide. The researcher should determine what protocol best meets their needs. Please follow safe laboratory procedures.


Immunohistochemistry-Paraffin Embedded Sections

Antigen Unmasking:
Bring slides to a boil in 10 mM sodium citrate buffer (pH 6.0) then maintain at a sub-boiling temperature for 10 minutes. Cool slides on bench-top for 30 minutes.

Staining:
1. Wash sections in deionized water three times for 5 minutes each.
2. Wash sections in wash buffer for 5 minutes.
3. Block each section with 100-400 ul blocking solution for 1 hour at room temperature.
4. Remove blocking solution and add 100-400 ul diluted primary antibody. Incubate overnight at 4C.
5. Remove antibody solution and wash sections in wash buffer three times for 5 minutes each.
6. Add 100-400 ul biotinylated diluted secondary antibody. Incubate 30 minutes at room temperature.
7. Remove secondary antibody solution and wash sections three times with wash buffer for 5 minutes each.
8. Add 100-400 ul Streptavidin-HRP reagent to each section and incubate for 30 minutes at room temperature.
9. Wash sections three times in wash buffer for 5 minutes each.
10. Add 100-400 ul DAB substrate to each section and monitor staining closely.
11. As soon as the sections develop, immerse slides in deionized water.
12. Counterstain sections in hematoxylin.
13. Wash sections in deionized water two times for 5 minutes each.
14. Dehydrate sections.
15. Mount coverslips.

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies