NCOR2 Antibody

Novus Biologicals | Catalog # NB120-5802

Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP)

Cited:

Simple Western

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all NCOR2 Primary Antibodies »

Product Specifications

Immunogen

His-tagged fusion protein corresponding to amino acid residues 95-565 from human SMRT.

Reactivity Notes

Cross-reacts with Human.Not yet tested in other species.

Localization

Nuclear

Specificity

NB120-5802 detects silencing mediator of retinoic acid and thyroid hormone receptor (SMRT) from human cells.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NCOR2 Antibody

Immunocytochemistry/ Immunofluorescence: NCOR2 Antibody [NB120-5802]

Immunocytochemistry/ Immunofluorescence: NCOR2 Antibody [NB120-5802]

Immunocytochemistry/Immunofluorescence: NCOR2 Antibody [NB120-5802] - Immunofluorescent analysis of NCoR2 (green) showing staining in the nucleus of U251 cells (right) compared to a negative control without primary antibody (left).
Immunohistochemistry: NCOR2 Antibody [NB120-5802]

Immunohistochemistry: NCOR2 Antibody [NB120-5802]

Immunohistochemistry: NCOR2 Antibody [NB120-5802] - Immunohistochemistry analysis of NCoR2 showing positive staining in the nucleus of paraffin-treated Human lung tissue (right) compared with a negative control in the absence of primary antibody (left).
Immunocytochemistry/ Immunofluorescence: NCOR2 Antibody [NB120-5802]

Immunocytochemistry/ Immunofluorescence: NCOR2 Antibody [NB120-5802]

Immunocytochemistry/Immunofluorescence: NCOR2 Antibody [NB120-5802] - Immunofluorescent analysis of NCoR2 (green) showing staining in the nucleus of Neuro-2a cells (right) compared to a negative control without primary antibody (left).
Immunocytochemistry/ Immunofluorescence: NCOR2 Antibody [NB120-5802]

Immunocytochemistry/ Immunofluorescence: NCOR2 Antibody [NB120-5802]

Immunocytochemistry/Immunofluorescence: NCOR2 Antibody [NB120-5802] - Immunofluorescent analysis of NCoR2 (green) showing staining in the nucleus of NIH-3T3 cells (right) compared to a negative control without primary antibody (left).
Immunocytochemistry/ Immunofluorescence: NCOR2 Antibody [NB120-5802]

Immunocytochemistry/ Immunofluorescence: NCOR2 Antibody [NB120-5802]

Immunocytochemistry/Immunofluorescence: NCOR2 Antibody [NB120-5802] - Immunofluorescent analysis of NCoR2 (green) showing staining in the nucleus of Hela cells (right) compared to a negative control without primary antibody (left).
Immunohistochemistry-Paraffin: NCOR2 Antibody [NB120-5802]

Immunohistochemistry-Paraffin: NCOR2 Antibody [NB120-5802]

Immunohistochemistry-Paraffin: NCOR2 Antibody [NB120-5802] - Immunohistochemistry analysis of NCoR2 showing positive staining in the nucleus of paraffin-treated Mouse brain tissue (right) compared with a negative control in the absence of primary antibody (left).

Applications for NCOR2 Antibody

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

1:10-1:500

Immunocytochemistry/ Immunofluorescence

1:100-1:200

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:10-1:500

Western Blot

4 ug/ml
Application Notes
WB: Use at a concentration of 4 ug/ml. Detects a band of approximately 250 kDa (predicted molecular weight: 302 kDa) representing SMRT from HeLa cell nuclear extracts.Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user.
See Simple Western Antibody Database for Simple Western validation: Tested in HSPCs, AML samples, separated by Size, apparent MW was ~270 kDa kDa

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS with 1 mg/ml BSA

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: NCOR2

Steroid and thyroid hormones and retinoic acid regulate a complex array of gene expression activity via intracellular receptor transcription factors belonging to the ligand-dependent nuclear receptor superfamily. Adding to the complexity of function of these transcription factors are associated proteins known as coactivators and corepressors which, as their names suggest, enhance or depress transcription activity of the nuclear receptor with which they associate. Silencing mediator of retinoic acid & thyroid hormone receptor (SMRT) and nuclear receptor corepressor (N-CoR) are related transcriptional corepressors which contain two distinct domains capable of interacting with unliganded nuclear receptors to repress their basal transcriptional activity.

Long Name

Nuclear Receptor Co-repressor 2

Alternate Names

CTG26, SMRT, SMRTE, TNRC14, TRAC-1

Entrez Gene IDs

9612 (Human)

Gene Symbol

NCOR2

Additional NCOR2 Products

Product Documents for NCOR2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for NCOR2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for NCOR2 Antibody

There are currently no reviews for this product. Be the first to review NCOR2 Antibody and earn rewards!

Have you used NCOR2 Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies

Associated Pathways

Notch Signaling Pathways Notch Signaling Pathway Thumbnail