NDRG2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-81424

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Cited:

Mouse

Predicted:

Rat (94%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all NDRG2 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: TGLTSSIPEMILGHLFSQEELSGNSELIQKYRNIITHAPNLDNIELYWNSYNNRRDLNFERGGDITLRCPVMLVVGDQAPHEDAVVECNSKLDPTQTSFLKMADSGGQP

Reactivity Notes

Mouse reactivity reported in the scientific literature (PMID: 26380811).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

41 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NDRG2 Antibody - BSA Free

Immunohistochemistry-Paraffin: NDRG2 Antibody [NBP1-81424]

Immunohistochemistry-Paraffin: NDRG2 Antibody [NBP1-81424]

Immunohistochemistry-Paraffin: NDRG2 Antibody [NBP1-81424] - Analysis in human salivary gland and pancreas tissues using NBP1-81424 antibody. Corresponding NDRG2 RNA-seq data are presented for the same tissues.
Western Blot: NDRG2 Antibody [NBP1-81424]

Western Blot: NDRG2 Antibody [NBP1-81424]

Western Blot: NDRG2 Antibody [NBP1-81424] - Analysis in human liver tissue.
Immunocytochemistry/ Immunofluorescence: NDRG2 Antibody [NBP1-81424]

Immunocytochemistry/ Immunofluorescence: NDRG2 Antibody [NBP1-81424]

Immunocytochemistry/Immunofluorescence: NDRG2 Antibody [NBP1-81424] - Staining of human cell line U-251 MG shows localization to nucleoplasm, cytosol and microtubule organizing center. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: NDRG2 Antibody [NBP1-81424]

Immunohistochemistry-Paraffin: NDRG2 Antibody [NBP1-81424]

Immunohistochemistry-Paraffin: NDRG2 Antibody [NBP1-81424] - Staining of human pancreas shows weak cytoplasmic positivity in exocrine glandular cells as expected.
Immunohistochemistry-Paraffin: NDRG2 Antibody [NBP1-81424]

Immunohistochemistry-Paraffin: NDRG2 Antibody [NBP1-81424]

Immunohistochemistry-Paraffin: NDRG2 Antibody [NBP1-81424] - Staining of human salivary gland shows strong cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: NDRG2 Antibody [NBP1-81424]

Immunohistochemistry-Paraffin: NDRG2 Antibody [NBP1-81424]

Immunohistochemistry-Paraffin: NDRG2 Antibody [NBP1-81424] - Staining of human breast shows strong cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: NDRG2 Antibody [NBP1-81424]

Immunohistochemistry-Paraffin: NDRG2 Antibody [NBP1-81424]

Immunohistochemistry-Paraffin: NDRG2 Antibody [NBP1-81424] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neuropil and glial cells.
Immunohistochemistry-Paraffin: NDRG2 Antibody [NBP1-81424]

Immunohistochemistry-Paraffin: NDRG2 Antibody [NBP1-81424]

Immunohistochemistry-Paraffin: NDRG2 Antibody [NBP1-81424] - Staining of human skeletal muscle shows moderate cytoplasmic positivity in myocytes.
Immunohistochemistry-Paraffin: NDRG2 Antibody [NBP1-81424]

Immunohistochemistry-Paraffin: NDRG2 Antibody [NBP1-81424]

Immunohistochemistry-Paraffin: NDRG2 Antibody [NBP1-81424] - Staining of human kidney shows moderate cytoplasmic positivity in cells in glomeruli.

Applications for NDRG2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:2500 - 1:5000

Immunohistochemistry-Paraffin

1:2500 - 1:5000

Western Blot

0.04 - 0.4 ug/ml
Application Notes
WB reported in scientific literature (PMID: 26380811). For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NDRG2

NDRG2 is a member of the N-myc downregulated gene family which belongs to the alpha/beta hydrolase superfamily. The protein encoded by this gene is a cytoplasmic protein that may play a role in neurite outgrowth. This gene may be involved in glioblastoma carcinogenesis. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.

Alternate Names

DKFZp781G1938, FLJ25522, KIAA1248cytoplasmic protein Ndr1, NDR1-related protein NDR2, NDRG family member 2, protein NDRG2, Protein Syld709613, syld709613 protein, SYLDN-myc downstream regulator 2

Entrez Gene IDs

57447 (Human)

Gene Symbol

NDRG2

UniProt

Q9UN36 (Human)

Additional NDRG2 Products

Product Documents for NDRG2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for NDRG2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for NDRG2 Antibody - BSA Free

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Protocols

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