NDUFS3 Antibody (HL2097)

Novus Biologicals | Catalog # NBP3-48671

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse, Rat, Zebrafish

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL2097
View all NDUFS3 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fragment of human NDUFS3

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NDUFS3 Antibody (HL2097)

NDUFS3 Antibody (HL2097)

Western Blot: NDUFS3 Antibody (HL2097) [NBP3-48671] -

Wild-type (WT) and NDUFS3 knockout (KO) 293T cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with NDUFS3 antibody [HL2097] (NBP3-48671) diluted at 1:2500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
NDUFS3 Antibody (HL2097)

Immunohistochemistry-Paraffin: NDUFS3 Antibody (HL2097) [NBP3-48671] -

NDUFS3 antibody [HL2097] detects NDUFS3 protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat tissues.
NDUFS3 stained by NDUFS3 antibody [HL2097] (NBP3-48671) diluted at 1:50.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
NDUFS3 Antibody (HL2097)

Immunocytochemistry/ Immunofluorescence: NDUFS3 Antibody (HL2097) [NBP3-48671] -

NDUFS3 antibody [HL2097] detects NDUFS3 protein at mitochondria by immunofluorescent analysis.
Sample: 293T cells were fixed in ice-cold MeOH for 5 min.
Green: NDUFS3 stained by NDUFS3 antibody [HL2097] (NBP3-48671) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.
Blue: Fluoroshield with DAPI.
NDUFS3 Antibody (HL2097)

Western Blot: NDUFS3 Antibody (HL2097) [NBP3-48671] -

Whole zebrafish extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with NDUFS3 antibody [HL2097] (NBP3-48671) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
NDUFS3 Antibody (HL2097)

Western Blot: NDUFS3 Antibody (HL2097) [NBP3-48671] -

Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with NDUFS3 antibody [HL2097] (NBP3-48671) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
NDUFS3 Antibody (HL2097)

Western Blot: NDUFS3 Antibody (HL2097) [NBP3-48671] -

Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with NDUFS3 antibody [HL2097] (NBP3-48671) diluted at 1:2500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
NDUFS3 Antibody (HL2097)

Immunohistochemistry-Paraffin: NDUFS3 Antibody (HL2097) [NBP3-48671] -

NDUFS3 antibody [HL2097] detects NDUFS3 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
NDUFS3 stained by NDUFS3 antibody [HL2097] (NBP3-48671) diluted at 1:50.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
NDUFS3 Antibody (HL2097)

Western Blot: NDUFS3 Antibody (HL2097) [NBP3-48671] -

Whole Japanese medaka extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with NDUFS3 antibody [HL2097] (NBP3-48671) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for NDUFS3 Antibody (HL2097)

Application
Recommended Usage

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NDUFS3

The multisubunit NADH:ubiquinone oxidoreductase (complex I) is the first enzyme complex in the electron transport chain of mitochondria. The iron-sulfur protein (IP) fraction of complex I is made up of 7 subunits. See NDUFS1 (MIM 157655).[supplied by OMIM]

Alternate Names

CI-30, CI-30kD, complex I 30kDa subunit, Complex I-30kD, EC 1.6.5.3, EC 1.6.99.3, EC 1.6.99.5, NADH dehydrogenase (ubiquinone) Fe-S protein 3 (30kD) (NADH-coenzyme Qreductase), NADH dehydrogenase (ubiquinone) Fe-S protein 3, 30kDa (NADH-coenzyme Qreductase), NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial, NADH dehydrogenase-ubiquinone 30 kDa subunit, NADH-ubiquinone oxidoreductase 30 kDa subunit

Gene Symbol

NDUFS3

Additional NDUFS3 Products

Product Documents for NDUFS3 Antibody (HL2097)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for NDUFS3 Antibody (HL2097)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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