Neogenin Antibody - BSA Free

Immunohistochemistry-Paraffin: Neogenin Antibody [NBP1-89651]

Immunohistochemistry-Paraffin: Neogenin Antibody [NBP1-89651] - Staining of human kidney shows moderate membranous positivity in cells in glomeruli.

Immunohistochemistry-Paraffin: Neogenin Antibody [NBP1-89651]

Immunohistochemistry-Paraffin: Neogenin Antibody [NBP1-89651] - Staining of human placenta shows no positivity in trophoblastic cells as expected.

Western Blot: Neogenin Antibody - BSA Free [NBP1-89651]

Analysis in human cell lines A-549 and HeLa using Anti-NEO1 antibody. Corresponding NEO1 RNA-seq data are presented for the same cell lines. Loading control: Anti-PARP1.

Western Blot: Neogenin Antibody - BSA Free [NBP1-89651] -

Expression profiling of spermatogonial stem cell markers in control and neogenin-cKO testes. Neogenin was conditionally knocked out in the mouse testis by CRISPR-Cas9 as described in the Materials and Methods. (A) Quantitative real-time PCR analysis of relative mRNA expression of neogenin, Oct3/4, Sox2, and Nanog normalized against that of beta -actin. (n = 3) (B) Proteins extracted from testicular tissues at postnatal day 26 were analyzed by Western blotting. beta -actin was used as a housekeeping protein. Con: control testis; Neo cKO: neogenin conditional knock-out testis. Data are the mean +/- SD of triplicates. * p < 0.05 and *** p < 0.001 versus control by the Student’s t-test. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36499089), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Neogenin Antibody - BSA Free [NBP1-89651] -

An ELISA of PGD2 in human follicular fluid and the culture medium of RGMc-treated CCs. (a) The PGD2 level in follicular fluid of patients with a POR and young and old patients with a normal ovarian response. (b,c) The ELISA data of PGD2 level in the culture media of RGMc-treated CCs obtained from (b) patients with a normal ovarian response and (c) patients with a POR. (d) Gene expression of neogenin in human CCs of normal and POR patients. All assays were repeated three times with different samples for statistical analysis. * p < 0.05 and *** p < 0.001 versus the young and old normal ovarian response groups. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33801938), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Neogenin Antibody - BSA Free [NBP1-89651] -

Involvement of RGMa in the osteogenic transdifferentiation of VSMCs in vitro. (A) Representative images of Alizarin red S staining and magnification. Red is the positive signal of calcification labelled by alizarin. (B) Representative images of RGMa and alpha -SMA immunofluorescence double labelling. alpha -SMA (green); RGMa (red). Scale bar = 50 μm. (C–I) Representative WB images of RGMa, Neogenin and vascular smooth muscle osteogenic transdifferentiation -related markers (C) and quantitative analysis of RGMa (D), Neogenin (E), Runx2 (F), BMP2 (G), alpha -SMA (H), SM22 alpha (I). n=6 per group, ns indicates not significant, *P < 0.05, **P < 0.01, ***P < 0.001, one-way ANOVA. Original blots/gels are available in Supplementary Figure S3. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/40634394), licensed under a CC-BY license. Not internally tested by Novus Biologicals.