Nephronophthisis Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-94059

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Nephronophthisis Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 463-732 of human Nephronophthisis (NP_997064.2). PDLGILFELGISYIRNSTGERGELSCGWVFLKLFDASGVPIPAKTYELFLNGGTPYEKGIEVDPSISRRAHGSVFYQIMTMRRQPQLLVKLRSLNRRSRNVLSLLPETLIGNMCSIHLLIFYRQILGDVLLKDRMSLQSTDLISHPMLATFPMLLEQPDVMDALRSSWAGKESTLKRSEKRDKEFLKSTFLLVYHDCVLPLLHSTRLPPFRWAEEETETARWKVITDFLKQNQENQGALQALLSPDGVHEPFDLSEQTYDFLGEMRKNAV

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Nephronophthisis Antibody - BSA Free

Western Blot: Nephronophthisis AntibodyAzide and BSA Free [NBP2-94059]

Western Blot: Nephronophthisis AntibodyAzide and BSA Free [NBP2-94059]

Western Blot: Nephronophthisis Antibody [NBP2-94059] - Analysis of extracts of Mouse pancreas, using NPHP1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 180s.
Immunohistochemistry-Paraffin: Nephronophthisis Antibody - Azide and BSA Free [NBP2-94059]

Immunohistochemistry-Paraffin: Nephronophthisis Antibody - Azide and BSA Free [NBP2-94059]

Immunohistochemistry-Paraffin: Nephronophthisis Antibody [NBP2-94059] - Rat testis using NPHP1 Rabbit pAb at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Western Blot: Nephronophthisis AntibodyAzide and BSA Free [NBP2-94059]

Western Blot: Nephronophthisis AntibodyAzide and BSA Free [NBP2-94059]

Western Blot: Nephronophthisis Antibody [NBP2-94059] - Analysis of extracts of Mouse pancreas, using NPHP1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 180s.
Immunohistochemistry-Paraffin: Nephronophthisis Antibody - Azide and BSA Free [NBP2-94059]

Immunohistochemistry-Paraffin: Nephronophthisis Antibody - Azide and BSA Free [NBP2-94059]

Immunohistochemistry-Paraffin: Nephronophthisis Antibody [NBP2-94059] - Rat kidney using NPHP1 Rabbit pAb at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Nephronophthisis Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: Nephronophthisis Antibody - Azide and BSA Free [Nephronophthisis] -

Immunocytochemistry/ Immunofluorescence: Nephronophthisis Antibody - Azide and BSA Free [Nephronophthisis] - Immunofluorescence analysis of NIH/3T3 cells using Nephronophthisis Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
Nephronophthisis Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: Nephronophthisis Antibody - Azide and BSA Free [Nephronophthisis] -

Immunocytochemistry/ Immunofluorescence: Nephronophthisis Antibody - Azide and BSA Free [Nephronophthisis] - Immunofluorescence analysis of U2OS cells using Nephronophthisis Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
Nephronophthisis Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: Nephronophthisis Antibody - Azide and BSA Free [Nephronophthisis] -

Immunocytochemistry/ Immunofluorescence: Nephronophthisis Antibody - Azide and BSA Free [Nephronophthisis] - Immunofluorescence analysis of PC-12 cells using Nephronophthisis Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Applications for Nephronophthisis Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Nephronophthisis

Nephronophthisis encodes a protein with src homology domain 3 (SH3) patterns. This protein interacts with Crk-associated substrate, and it appears to function in the control of cell division, as well as in cell-cell and cell-matrix adhesion signaling, likely as part of a multifunctional complex localized in actin- and microtubule-based structures. Mutations in this gene cause familial juvenile nephronophthisis type 1, a kidney disorder involving both tubules and glomeruli. Defects in this gene are also associated with Senior-Loken syndrome type 1, also referred to as juvenile nephronophthisis with Leber amaurosis, which is characterized by kidney and eye disease, and with Joubert syndrome type 4, which is characterized by cerebellar ataxia, oculomotor apraxia, psychomotor delay and neonatal breathing abnormalities, sometimes including retinal dystrophy and renal disease. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Alternate Names

FLJ97602, JBTS4NPH1Juvenile nephronophthisis 1 protein, nephrocystin 1, nephrocystin-1, nephronophthisis 1 (juvenile), SLSN1

Gene Symbol

NPHP1

Additional Nephronophthisis Products

Product Documents for Nephronophthisis Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Nephronophthisis Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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