Neurogenin-3 Antibody (OTI3B5)

Novus Biologicals | Catalog # NBP1-47878

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI3B5
View all Neurogenin-3 Primary Antibodies »

Product Specifications

Immunogen

Full-length protein expressed in 293T cell transfected with human NEUROG3 expression vector

Specificity

This antibody is specific for Homo sapiens neurogenin 3 (NEUROG3).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

23.077 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Neurogenin-3 Antibody (OTI3B5)

Western Blot: Neurogenin-3 Antibody (OTI3B5) [NBP1-47878]

Western Blot: Neurogenin-3 Antibody (OTI3B5) [NBP1-47878]

Western Blot: Neurogenin-3 Antibody (OTI3B5) [NBP1-47878] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Neurogenin-3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Neurogenin-3.
Immunocytochemistry/ Immunofluorescence: Neurogenin-3 Antibody (OTI3B5) [NBP1-47878]

Immunocytochemistry/ Immunofluorescence: Neurogenin-3 Antibody (OTI3B5) [NBP1-47878]

Immunocytochemistry/Immunofluorescence: Neurogenin-3 Antibody (OTI3B5) [NBP1-47878] - Neurogenin3 Immunofluorescent staining of HeLa cells transiently transfected by pCMV6-ENTRY Neurogenin-3.
Immunohistochemistry-Paraffin: Neurogenin-3 Antibody (OTI3B5) [NBP1-47878]

Immunohistochemistry-Paraffin: Neurogenin-3 Antibody (OTI3B5) [NBP1-47878]

Immunohistochemistry-Paraffin: Neurogenin-3 Antibody (OTI3B5) [NBP1-47878] - Staining of paraffin-embedded Human liver tissue within the normal limits using anti-NEUROG3 mouse monoclonal antibody at 1:150 dilution.
Immunohistochemistry-Paraffin: Neurogenin-3 Antibody (OTI3B5) [NBP1-47878]

Immunohistochemistry-Paraffin: Neurogenin-3 Antibody (OTI3B5) [NBP1-47878]

Immunohistochemistry-Paraffin: Neurogenin-3 Antibody (OTI3B5) [NBP1-47878] - Staining of paraffin-embedded Human lymph node tissue using anti-Neurogenin-3 mouse monoclonal antibody.
Immunoprecipitation: Neurogenin-3 Antibody (OTI3B5) [NBP1-47878]

Immunoprecipitation: Neurogenin-3 Antibody (OTI3B5) [NBP1-47878]

Immunoprecipitation: Neurogenin-3 Antibody (OTI3B5) [NBP1-47878] - (Negative control: IP without adding anti-NEUROG3 antibody). For each experiment, 500ul of DDK tagged NEUROG3 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-NEUROG3 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

Applications for Neurogenin-3 Antibody (OTI3B5)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Immunohistochemistry-Paraffin

1:50

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Neurogenin-3

Neurogenin-3 (NEUROG3) is expressed in endocrine progenitor cells and is required for endocrine cell development in the pancreas and intestine (Wang et al., 2006 [PubMed 16855267]). It belongs to a family of basic helix-loop-helix transcription factors involved in the determination of neural precursor cells in the neuroectoderm

Alternate Names

Atoh5, Math4B, NEUROG3, Neurogenin3, Relax

Entrez Gene IDs

50674 (Human)

Gene Symbol

NEUROG3

Additional Neurogenin-3 Products

Product Documents for Neurogenin-3 Antibody (OTI3B5)

Certificate of Analysis

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Product Specific Notices for Neurogenin-3 Antibody (OTI3B5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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