Neurogenin-3 Antibody (OTI3B5) - Azide and BSA Free
Novus Biologicals | Catalog # NBP2-72956
![Western Blot: Neurogenin-3 Antibody (OTI3B5)Azide and BSA Free [NBP2-72956]](https://resources.rndsystems.com/images/products/Neurogenin-3-Antibody-OTI3B5-Azide-and-BSA-Free-Western-Blot-NBP2-72956-img0001.jpg "Western Blot: Neurogenin-3 Antibody (OTI3B5)Azide and BSA Free [NBP2-72956]")
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # OTI3B5
Format
Azide and BSA Free
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Product Specifications
Immunogen
Full-length protein expressed in 293T cell transfected with human NEUROG3 expression vector
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Theoretical MW
23.077 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Neurogenin-3 Antibody (OTI3B5) - Azide and BSA Free
Western Blot: Neurogenin-3 Antibody (OTI3B5)Azide and BSA Free [NBP2-72956]
Western Blot: Neurogenin-3 Antibody (OTI3B5) - Azide and BSA Free [NBP2-72956] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Neurogenin-3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Neurogeni![Immunohistochemistry: Neurogenin-3 Antibody (OTI3B5) - Azide and BSA Free [NBP2-72956]](https://resources.rndsystems.com/images/products/Neurogenin-3-Antibody-OTI3B5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72956-img0004.jpg "Immunohistochemistry: Neurogenin-3 Antibody (OTI3B5) - Azide and BSA Free [NBP2-72956]")
Immunohistochemistry: Neurogenin-3 Antibody (OTI3B5) - Azide and BSA Free [NBP2-72956]
Immunohistochemistry: Neurogenin-3 Antibody (OTI3B5) - Azide and BSA Free [NBP2-72956] - Staining of paraffin-embedded Human lymph node tissue using anti-Neurogenin-3 mouse monoclonal antibody.![Flow Cytometry: Neurogenin-3 Antibody (OTI3B5) - Azide and BSA Free [NBP2-72956]](https://resources.rndsystems.com/images/products/Neurogenin-3-Antibody-OTI3B5-Azide-and-BSA-Free-Flow-Cytometry-NBP2-72956-img0002.jpg "Flow Cytometry: Neurogenin-3 Antibody (OTI3B5) - Azide and BSA Free [NBP2-72956]")
Flow Cytometry: Neurogenin-3 Antibody (OTI3B5) - Azide and BSA Free [NBP2-72956]
Flow Cytometry: Neurogenin-3 Antibody (OTI3B5) - Azide and BSA Free [NBP2-72956] - Neurogenin3 Staining of HeLa cells transiently transfected by pCMV6-ENTRY Neurogenin-3.![Immunohistochemistry: Neurogenin-3 Antibody (OTI3B5) - Azide and BSA Free [NBP2-72956]](https://resources.rndsystems.com/images/products/Neurogenin-3-Antibody-OTI3B5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72956-img0003.jpg "Immunohistochemistry: Neurogenin-3 Antibody (OTI3B5) - Azide and BSA Free [NBP2-72956]")
Immunohistochemistry: Neurogenin-3 Antibody (OTI3B5) - Azide and BSA Free [NBP2-72956]
Immunohistochemistry: Neurogenin-3 Antibody (OTI3B5) - Azide and BSA Free [NBP2-72956] - Staining of paraffin-embedded Human liver tissue within the normal limits using anti-NEUROG3 mouse monoclonal antibody at 1:150 dilution.![Immunoprecipitation: Neurogenin-3 Antibody (OTI3B5) - Azide and BSA Free [NBP2-72956]](https://resources.rndsystems.com/images/products/Neurogenin-3-Antibody-OTI3B5-Azide-and-BSA-Free-Immunoprecipitation-NBP2-72956-img0005.jpg "Immunoprecipitation: Neurogenin-3 Antibody (OTI3B5) - Azide and BSA Free [NBP2-72956]")
Immunoprecipitation: Neurogenin-3 Antibody (OTI3B5) - Azide and BSA Free [NBP2-72956]
Immunoprecipitation: Neurogenin-3 Antibody (OTI3B5) - Azide and BSA Free [NBP2-72956] - (Negative control: IP without adding anti-NEUROG3 antibody). For each experiment, 500ul of DDK tagged NEUROG3 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-NEUROG3 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.Applications for Neurogenin-3 Antibody (OTI3B5) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100
Immunohistochemistry
1:50 - 1:150
Western Blot
1:2000
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Reconstitution
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.
Formulation
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Calculators
Background: Neurogenin-3
Alternate Names
Atoh5, Math4B, NEUROG3, Neurogenin3, Relax
Gene Symbol
NEUROG3
Additional Neurogenin-3 Products
Product Documents for Neurogenin-3 Antibody (OTI3B5) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Neurogenin-3 Antibody (OTI3B5) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
