Neurokinin B Antibody - BSA Free

Novus Biologicals | Catalog # NB300-201

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Neurokinin B Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to an internal sequence of mouse proNeurokinin B (~93% homology to the rat sequence). [UniProt# P55099]

Localization

Secreted

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Neurokinin B Antibody - BSA Free

Immunohistochemistry: Neurokinin B Antibody - BSA Free [NB300-201]

Immunohistochemistry: Neurokinin B Antibody - BSA Free [NB300-201]

Neurokinin-B-Antibody---BSA-Free-Immunohistochemistry-NB300-201-img0009.jpg
Immunohistochemistry-Paraffin: Neurokinin B Antibody - BSA Free [NB300-201]

Immunohistochemistry-Paraffin: Neurokinin B Antibody - BSA Free [NB300-201]

Immunohistochemistry-Paraffin: Neurokinin B Antibody [NB300-201] - IHC analysis of formalin fixed paraffin embedded tissue section of mouse brain using Neurokinin B antibody at 1:200 dilution. The antibody depicted an expected strong cytoplasmic staining in the neuronal cells.
Immunohistochemistry-Paraffin: Neurokinin B Antibody - BSA Free [NB300-201]

Immunohistochemistry-Paraffin: Neurokinin B Antibody - BSA Free [NB300-201]

Immunohistochemistry-Paraffin: Neurokinin B Antibody [NB300-201] - IHC analysis of formalin fixed paraffin embedded tissue section of mouse brain using Neurokinin B antibody at 1:100 dilution. The antibody showed a specific cytoplasmic staining in the Purkinje cells and some granular neurons from the granular layer with weak signal in the fibers within the molecular layer of the section.
Immunohistochemistry: Neurokinin B Antibody - BSA Free [NB300-201]

Immunohistochemistry: Neurokinin B Antibody - BSA Free [NB300-201]

Immunohistochemistry: Neurokinin B Antibody [NB300-201] - IF analysis of Neurokinin B in mouse hypothalamus. Image courtesy of an anonymous customer review.
Immunohistochemistry: Neurokinin B Antibody - BSA Free [NB300-201]

Immunohistochemistry: Neurokinin B Antibody - BSA Free [NB300-201]

Immunohistochemistry: Neurokinin B Antibody [NB300-201] - Detection of Neurokinin B in rat brain tissue (Alexa 488 stain), using NB 300-201.
Neurokinin B Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Neurokinin B Antibody - BSA Free [NB300-201] -

Immunocytochemistry/ Immunofluorescence: Neurokinin B Antibody - BSA Free [NB300-201] - Troxerutin reduced the expression of Kisspepetin1 & Neurokinin B in the ARC. a Immunofluorescence photomicrograph showing that the expression of Kisspeptin1 & NKB in the arcuate nucleus (ARC). Scale bars:20 μm. b A lower magnification image showing the whole ARC & median eminence. Rectangular frame a & b represent ARC & median eminence (ME) analyzed in Fig. 5a & Fig. 6a, respectively. Scale bars:200 μm. c Quantitative analysis of integral optical density (IOD) of Kisspeptin1 & NKB in the ARC. P values were determined by one-way ANOVA with Tukey’s multiple comparison test & data were presented as means±s.e.m. n = 4 per group. **P < 0.01, ***P < 0.001vs. sham group; #P < 0.05, ##P < 0.01 vs. PCOS group Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32921318), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Neurokinin B Antibody - BSA Free

Immunohistochemistry: Neurokinin B Antibody - BSA Free [NB300-201] -

Immunohistochemistry: Neurokinin B Antibody - BSA Free [NB300-201] - Characterization of Kiss1-null mice.(A-C) Immunofluorescence for kisspeptin (A,B) & NKB (C) in the ARN of wild-type (A) & Kiss1-null (B,C) female mice. (D) Cell-attached recordings showing the effects of 100 nM NKB on firing of KNDy neurons in acute brain slices prepared from female heterozygous Kiss1Cre/+ and homozygous (null) Kiss1Cre/Cre;Rosa26-tdT mice. (E) Mean ± SEM changes in KNDy neuron firing evoked by 100 nM NKB in heterozygous Kiss1Cre/+and homozygous (null) Kiss1Cre/Cre;Rosa26-tdT mice. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33464205), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Neurokinin B Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Frozen

1:500-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:100-1:2000
Application Notes
This Neurokinin B antibody is useful for Immunocytochemistry/Immunofluorescence and Immunohistochemistry on paraffin-embedded and frozen sections. This antibody has been used on human hypothalamus and rat brain sections. In IHC, positive staining of the arcuate nucleus is seen. There has been customer feedback that this antibody works in Western blot; please note that this is a secreted protein.

Reviewed Applications

Read 2 reviews rated 4 using NB300-201 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Neurokinin B

Neurokinin B (NKB) belongs to tachykinin family of peptides that also includes substance P, NKA, neuropeptide K, neuropeptide-gamma, and hemokinin-1, and NKB is the only tachykinin synthesized from preprotachykinin-B gene (designated as TAC3 in mammals, except for rodents where it is named Tac2). Tachykinins are characterized by a common carboxyl-terminal amino acid sequence of FXGLM-NH2, X being a hydrophobic residue and are active peptides which stimulates neurons, evoke behavioral responses, are potent vasodilators and secretagogues (secretion stimulators), and contract directly or indirectly smooth muscles. NKB is the cleaved product of neurokinin B-like protein Zneurok1 (also called preprotachykinin-B or tachykinin-3) and act as an endogenous agonist ligand for its receptor, the NK3 receptor (NKB receptor). NKB signaling has been reported to play critical role in pre-eclampsia which is a potentially life threatening disease and is essential to neuroprotection in neurodegenerative diseases as well as to normal reproductive development.

Alternate Names

gamma tachykinin 3, neurokinin beta, neurokinin B-like, neuromedin K, preprotachykinin B, PRO1155, tachykinin 3, tachykinin-3, ZNEUROK1NKNBNKB

Entrez Gene IDs

6866 (Human); 21334 (Mouse); 29191 (Rat)

Gene Symbol

TAC3

UniProt

P55099 (Mouse)

Additional Neurokinin B Products

Product Documents for Neurokinin B Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Neurokinin B Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Neurokinin B Antibody - BSA Free

Customer Reviews for Neurokinin B Antibody - BSA Free (2)

4 out of 5
2 Customer Ratings
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Customer Images

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  • Name: Andree Lessard
    Application: Immunohistochemistry
    Sample Tested: rat tissue fixed with PFA and acrolein
    Species: Rat
    Verified Customer | Posted 06/22/2012
    light micrograph of rat hypothalamic and midbrain tissue single immunolabeled with NK3 antisera
    Neurokinin B Antibody - BSA Free NB300-201
  • Name: Christina Merkley
    Application: Immunofluorescence
    Sample Tested: Mouse hypothalamus
    Species: Mouse
    Verified Customer | Posted 05/15/2012
    Amplified fluorescence (BT, Cy5) for NKB in the ARC of a female mouse using NB 300-201
    Neurokinin B Antibody - BSA Free NB300-201

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Protocols

View specific protocols for Neurokinin B Antibody - BSA Free (NB300-201):

Immunohistochemistry-Paraffin Embedded Sections

Antigen Unmasking:
Bring slides to a boil in 10 mM sodium citrate buffer (pH 6.0) then maintain at a sub-boiling temperature for 10 minutes. Cool slides on bench-top for 30 minutes (keep slides in the sodium citrate buffer at all times).

Staining:
1. Wash sections in deionized water three times for 5 minutes each.
2. Wash sections in PBS for 5 minutes.
3. Block each section with 100-400 ul blocking solution (1% BSA in PBS) for 1 hour at room temperature.
4. Remove blocking solution and add 100-400 ul diluted primary antibody. Incubate overnight at 4 C.
5. Remove antibody solution and wash sections in wash buffer three times for 5 minutes each.
6. Add 100-400 ul HRP polymer conjugated secondary antibody. Incubate 30 minutes at room temperature.
7. Wash sections three times in wash buffer for 5 minutes each.
8. Add 100-400 ul DAB substrate to each section and monitor staining closely.
9. As soon as the sections develop, immerse slides in deionized water.
10. Counterstain sections in hematoxylin.
11. Wash sections in deionized water two times for 5 minutes each.
12. Dehydrate sections.
13. Mount coverslips.


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FAQs for Neurokinin B Antibody - BSA Free

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  • Q: Has NB300-201 been tested by IHC on frozen of paraffin embedded tissue section?

    A: This antibody is suitable for use on both frozen and paraffin-embedded tissues.

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