Neuromedin-U Antibody (HL3549)

Novus Biologicals | Catalog # NBP3-48826

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL3549
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Product Specifications

Immunogen

Recombinant fragment of human Neuromedin-U.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Neuromedin-U Antibody (HL3549)

Neuromedin-U Antibody (HL3549)

Immunocytochemistry/ Immunofluorescence: Neuromedin-U Antibody (HL3549) [NBP3-48826] -

Neuromedin-U antibody [HL3549] detects Neuromedin-U protein by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Neuromedin-U stained by Neuromedin-U antibody [HL3549] (NBP3-48826) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.
Blue: Fluoroshield with DAPI.
Neuromedin-U Antibody (HL3549)

Western Blot: Neuromedin-U Antibody (HL3549) [NBP3-48826] -

K562 whole cell extract and conditioned medium (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Neuromedin-U antibody [HL3549] (NBP3-48826) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Neuromedin-U Antibody (HL3549)

Western Blot: Neuromedin-U Antibody (HL3549) [NBP3-48826] -

Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Neuromedin-U antibody [HL3549] (NBP3-48826) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Applications for Neuromedin-U Antibody (HL3549)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Neuromedin-U

Neuromedin-U (NMU) is a member of the NmU family. NMU is responsible for muscle contractions in specific regions of the gastrointestinal tract. In humans, these regions include the ileum and urinary bladder.

Alternate Names

NMU

Gene Symbol

NMU

Additional Neuromedin-U Products

Product Documents for Neuromedin-U Antibody (HL3549)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Neuromedin-U Antibody (HL3549)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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