Neuropeptide Y Antibody

Novus Biologicals | Catalog # NBP1-46535

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Rabbit, Xenopus

Cited:

Human, Mouse, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunohistochemistry Free-Floating, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry Free-Floating, Western Blot, Immunofluorescence, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Neuropeptide Y/NPY Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide from amino acid region 20-70 of mouse Neuropeptide Y conjugated to an immunogenic carrier protein was used as the immunogen. The antigen is homologous in many other species including human, rat, zebrafish and xenopus.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Neuropeptide Y Antibody

Immunohistochemistry: Neuropeptide Y Antibody [NBP1-46535]

Immunohistochemistry: Neuropeptide Y Antibody [NBP1-46535]

Neuropeptide-Y-Antibody-Immunohistochemistry-NBP1-46535-img0001.jpg
Neuropeptide Y Antibody

Immunocytochemistry/ Immunofluorescence: Neuropeptide Y Antibody [NBP1-46535] -

Immunocytochemistry/ Immunofluorescence: Neuropeptide Y Antibody [NBP1-46535] - NHE1 distribution (A) & colocalisation with markers for specific cell types (B–D) & major inputs (E, F, G). (A) NHE1 immunoreactivity is distributed throughout the rostrocaudal axis of the SCN (encircled by the dotted lines). Scale bar: 200 µm. OC: optic chiasm. 3 V: third ventricle. (B–G1) Low magnification images showing the double staining pattern of NHE1 with neuropeptides NP2 (B), GRP (C), & VIP (D) as well as markers for afferent inputs vGluT2 (E), NPY (F), & SERT (G1). Scale bar: 100 µm. Insets: High magnification images showing individual cells with double staining. Scale bar: 10 µm. Asterisks mark Hoechst-stained nuclei. (G2) High magnification image showing high degree of colocalisation (yellow) between NHE1 (green) & SERT (red). Scale bar: 10 µm. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31015514), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Neuropeptide Y Antibody

Application
Recommended Usage

Immunohistochemistry

1:300-1:3000

Immunohistochemistry-Frozen

1:300-1:3000

Immunohistochemistry-Paraffin

1:300-1:3000

Western Blot

1:300-1:3000
Application Notes
Use in ICC/IF reported in scientific literature (PMID: 27612517). Use in IHC-FrFl reported in scientific literature (PMID: 31609204).

Formulation, Preparation, and Storage

Purification

Unpurified

Reconstitution

Reconstitute in 150 ul of sterile water. Centrifuge to remove any insoluble material. Glycerol may be added (1:1) for additional stability. Please note the sample size is provided in reconstituted format.

Formulation

Lyophilized from whole antisera

Preservative

No Preservative

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Neuropeptide Y/NPY

Neuropeptide Y (NPY) is a 36-amino-acid residue peptide with sequence and 3-D structural homology with PYY and pancreatic polypeptide (PP). NPY serves as a neurotransmitter and is exclusively localized in neurons in the central and peripheral nervous system. NPY is associated with the regulation of heart rate and food intake in several mammalian species.

Alternate Names

NPY, PYY4

Entrez Gene IDs

4852 (Human); 109648 (Mouse); 24604 (Rat)

Gene Symbol

NPY

UniProt

P57774 (Mouse)

Additional Neuropeptide Y/NPY Products

Product Documents for Neuropeptide Y Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Neuropeptide Y Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Neuropeptide Y Antibody

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Protocols

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FAQs

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