Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot, Flow Cytometry, Immunocytochemistry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 950334

Format

Azide and BSA Free
View all Neutrophil Elastase/ELA2 Primary Antibodies »

Product Specifications

Immunogen

Partial recombinant human ELA2 protein (aa28-252)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Neutrophil Elastase/ELA2 Antibody (950334) - Azide and BSA Free

Western Blot: Neutrophil Elastase/ELA2 Antibody (950334)Azide and BSA Free [MAB9167]

Western Blot: Neutrophil Elastase/ELA2 Antibody (950334)Azide and BSA Free [MAB9167]

Western Blot: Neutrophil Elastase/ELA2 Antibody (950334) - Azide and BSA Free [MAB9167] - Neutrophil Elastase/ELA2 Antibody (950334) [MAB9167] - Analysis of human bone marrow tissue lysate with 0.5 ug/ml of hELA-2 Monoclonal Antibody followed by HRP-conjugated Anti-Mouse IgG Antibody. A specific band was detected for hELA-2 at approximately 25-30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry: Neutrophil Elastase/ELA2 Antibody (950334) - Azide and BSA Free [MAB9167]

Immunocytochemistry: Neutrophil Elastase/ELA2 Antibody (950334) - Azide and BSA Free [MAB9167]

Immunocytochemistry: Neutrophil Elastase/ELA2 Antibody (950334) - Azide Free [MAB9167] - Neutrophil Elastase/ELA2 was detected in immersion fixed THP-1 cell using Neutrophil Elastase/ELA2 Monoclonal Antibody at 8 ug/ml dilution. Cells were stained using Donkey anti-Mouse IgG Secondary Antibody [NL557] (red) and counterstained with DAPI (blue). Specific staining was localized to cytoplasmic.
Immunohistochemistry: Neutrophil Elastase/ELA2 Antibody (950334) - Azide and BSA Free [MAB9167]

Immunohistochemistry: Neutrophil Elastase/ELA2 Antibody (950334) - Azide and BSA Free [MAB9167]

Immunohistochemistry: Neutrophil Elastase/ELA2 Antibody (950334) [MAB9167] - ELA-2 was detected in immersion fixed paraffin-embedded sections of human lymphoma using ELA-2 Monoclonal Antibody at 5 ug/ml. Tissue was stained using Mouse IgG VisUCyte HRP Polymer Antibody (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neutrophils.
Flow Cytometry: Neutrophil Elastase/ELA2 Antibody (950334) - Azide and BSA Free [MAB9167]

Flow Cytometry: Neutrophil Elastase/ELA2 Antibody (950334) - Azide and BSA Free [MAB9167]

Flow Cytometry: Neutrophil Elastase/ELA2 Antibody (950334) - Azide Free [MAB9167] - THP-1 human acute monocytic leukemia cell line treated with 3 uM monensin for 3 hours was stained with Mouse Anti-Human Neutrophil Elastase/ELA2 Monoclonal Antibody (Catalog # MAB9167, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005).

Applications for Neutrophil Elastase/ELA2 Antibody (950334) - Azide and BSA Free

Application
Recommended Usage

CyTOF-ready

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This antibody is Cytof ready.

Reviewed Applications

Read 1 review rated 5 using MAB9167 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Neutrophil Elastase/ELA2

The ELANE gene (commonly known as neutrophil elastase) encodes a 267 amino acid long, 28 kDA neutrophil elastase protein that functions in regulation of natural killer cells, monocytes, and graulocytes. ELANE is involved in activation of proMMP8, cell adhesion cell-matrix glycoconjugates, selected targets of C/EBPbeta, degradation of the extracellular matrix and collagen, and amb2 integrin signaling. It interacts with various genes GZMB, LRP1, SERPINF2, SERPING1, and SERPINA1. Elane has been linked to cystic fibrosis, asthma, pneumonia, alzheimer's disease, neutropenia, cutis laxia, leg ulcers, wegener's granulomatosis, bronchitis, adult respiratory distress syndrome, and vascular diseases.

Alternate Names

ELA2, ELANE, Elastase-2, Leukocyte Elastase, Medullasin

Gene Symbol

ELANE

Additional Neutrophil Elastase/ELA2 Products

Product Documents for Neutrophil Elastase/ELA2 Antibody (950334) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Neutrophil Elastase/ELA2 Antibody (950334) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Neutrophil Elastase/ELA2 Antibody (950334) - Azide and BSA Free

Customer Reviews for Neutrophil Elastase/ELA2 Antibody (950334) - Azide and BSA Free (1)

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  • Neutrophil Elastase/ELA2 Antibody (950334) - Azide Free
    Name: Manuel Flores
    Application: Immunohistochemistry
    Sample Tested: Liver cancer tissue
    Species: Human
    Verified Customer | Posted 06/09/2017
    Neutrophil elastase (NE) IF in magenta on FFPE section of human liver. Primary Ab is MAB9167 from Novus Biologicals. Secondary Ab is donkey anti-mouse Dylight 755. Cells morphology correspond to neutrophils with segmented nuclei.
    IHC. Single staining of neutrophil elastase (NE) in magenta on FFPE section of human liver cancer. Primary Ab is MAB9167 from Novus Biologicals. Secondary Ab is donkey anti-mouse Dylight 755. The cells exhibit a morphology that correspond to neutrophils with segmented nuclei.

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