NF-H Antibody - BSA Free

Novus Biologicals | Catalog # NB300-217

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Bovine, Canine, Equine, Feline

Cited:

Mouse, Rat, Feline

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunohistochemistry Free-Floating, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Chicken IgY

Format

BSA Free
View all NF-H Primary Antibodies »

Product Specifications

Immunogen

Native NF-H (phosphorylated) purified from bovine spinal cord.

Reactivity Notes

Feline reactivity reported in verified customer review. Feline reactivity reported in scientific literature (PMID:33091431).

Marker

Neuronal Marker

Specificity

Reacts very strongly with NF-H KSP type phosphorylated repeats. Reactivity with non-phosphorylated KSP sequences is orders of magnitude weaker.

Clonality

Polyclonal

Host

Chicken

Isotype

IgY

Theoretical MW

200-220 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NF-H Antibody - BSA Free

Western Blot: NF-H Antibody [NB300-217]

Western Blot: NF-H Antibody [NB300-217]

Western Blot: NF-H Antibody [NB300-217] - Analysis of spinal cord lysates from different species using chicken pAb to NF-H, NB300-217, dilution 1:20,000 in green: [1] protein standard (red), [2] rat, [3] mouse, and [4] cow spinal cord. Strong band at about 200-220kDa corresponds to the phosphorylated from of NF-H. The protein from different species is known to have different SDS-PAGE molecular weights, with large species generally expressing larger proteins. Smaller proteolytic fragments of NF-H are also detected in spinal cord preparations with this antibody. The antibody does not recognize non-phosphorylated forms of NF-H.
Immunocytochemistry/ Immunofluorescence: NF-H Antibody [NB300-217]

Immunocytochemistry/ Immunofluorescence: NF-H Antibody [NB300-217]

Immunocytochemistry/Immunofluorescence: NF-H Antibody [NB300-217] - Imaging of Feline optic nerve at antibody dilution 1:5000. This image was submitted via customer Review.
Immunohistochemistry Free-Floating: NF-H Antibody [NB300-217]

Immunohistochemistry Free-Floating: NF-H Antibody [NB300-217]

Immunohistochemistry Free-Floating: NF-H Antibody [NB300-217] - Analysis of a rat cerebellum section stained with NF-H antibody, dilution 1:5,000 (Red), and costained with rabbit GFAP pAb, dilution 1:5,000 Green). DAPI staining of nuclear DNA (Blue). Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24hrs, cut to 45uM, and free floating sections were stained with above antibodies. The NF-H antibody labels network of axons of different neurons, while the GFAP antibody stains astrocytes and other glial cells.
Western Blot: NF-H Antibody [NB300-217]

Western Blot: NF-H Antibody [NB300-217]

Western Blot: NF-H Antibody [NB300-217] - Analysis of 200kDa Neurofilament Heavy expression in rat spinal cord extract. The first lane is Coomassie Brilliant Blue stained and the second lane is probed with chicken anti-Neurofilament Heavy antibody NB300-217.
Immunocytochemistry/ Immunofluorescence: NF-H Antibody [NB300-217]

Immunocytochemistry/ Immunofluorescence: NF-H Antibody [NB300-217]

Immunocytochemistry/Immunofluorescence: NF-H Antibody [NB300-217] - Rat neurons stained with 200kDa Neurofilament Heavy Antibody NB300-217.
Immunocytochemistry/ Immunofluorescence: NF-H Antibody [NB300-217]

Immunocytochemistry/ Immunofluorescence: NF-H Antibody [NB300-217]

Immunocytochemistry/Immunofluorescence: NF-H Antibody [NB300-217] - SH-SY5Y cells stained with 200kDa Neurofilament Heavy Antibody NB300-217 (red) and Fibrillarin Antibody NB300-269 (green). Nuclear DNA is stained with Hoechst dye (blue).
Immunocytochemistry/ Immunofluorescence: NF-H Antibody [NB300-217]

Immunocytochemistry/ Immunofluorescence: NF-H Antibody [NB300-217]

Immunocytochemistry/Immunofluorescence: NF-H Antibody [NB300-217] - Mixed rat neuron/glial cultures stained with Neurofilament Light (NF-L) antibody NBP1-05217 [green] and Neurofilament Heavy (NF-H) antibody NB300-217 [red]. Blue is a DNA stain. NB300-217 binds primarily to the phosphorylated axonal forms of NF-H, in contrast to NBP1-05217 which stains both axonal and dendritic/perikaryal neurofilaments. The surrounding axonal profiles are orange due to staining of both the NF-H and NF-L antibodies.
Immunohistochemistry-Frozen: NF-H Antibody [NB300-217]

Immunohistochemistry-Frozen: NF-H Antibody [NB300-217]

Immunohistochemistry-Frozen: NF-H Antibody [NB300-217] - Canine optic nerve, antibody specifically labelled the axons (Green). Blue is DAPI. Image taken with an epifluorescent microscope and was incubated at 1:10000 for 1hr at RT.. Image from verified customer review.
NF-H Antibody

Immunohistochemistry-Paraffin: NF-H Antibody [NB300-217] -

Immunohistochemistry-Paraffin: NF-H Antibody [NB300-217] - NF-H immunoreactivity in a FFPE section of mouse trigeminal ganglion. Antibody was diluted 1 to 2000 and left on sections for 1h at room temperature. Image from verified customer review.
NF-H Antibody

Immunohistochemistry-Frozen: Chicken Polyclonal NF-H Antibody [NB300-217] -

Immunohistochemistry-Frozen: Chicken Polyclonal NF-H Antibody [NB300-217] - Fixed frozen section of mouse optic nerve showing neurofilament heavy NB300-217 in green and S100B in red. NB300-217 was diluted 1 in 4000 and was left on tissue sections overnight at room temperature. Primary was detected with donkey anti chicken conjugated to Alexa 488. Image from a verified customer review.
NF-H Antibody

Immunohistochemistry-Paraffin: Chicken Polyclonal NF-H Antibody [NB300-217] -

Immunohistochemistry-Paraffin: Chicken Polyclonal NF-H Antibody [NB300-217] - FFPE section of canine cecum showing NF-H NB300-217 immunoreactivity in enteric neurons. Primary antibody was diluted 1 in 2000 and left on tissue sections for 30m at room temperature. Secondary was donkey anti chicken HRP. Image from a verified customer review.
NF-H Antibody

Immunohistochemistry-Paraffin: Chicken Polyclonal NF-H Antibody [NB300-217] -

Immunohistochemistry-Paraffin: Chicken Polyclonal NF-H Antibody [NB300-217] - FFPE section of feline colon showing NF-H NB300-217 immunoreactivity in enteric neurons. Primary antibody was diluted 1 in 2000 and left on tissue sections for 30m at room temperature. Secondary was donkey anti chicken HRP. Image from a verified customer review.
NF-H Antibody

Immunohistochemistry-Paraffin: Chicken Polyclonal NF-H Antibody [NB300-217] -

Immunohistochemistry-Paraffin: Chicken Polyclonal NF-H Antibody [NB300-217] - FFPE section of equine jejunum showing NF-H NB300-217 immunoreactivity in enteric neurons. Primary antibody was diluted 1 in 2000 and left on tissue sections for 30m at room temperature. Secondary was donkey anti chicken HRP. Image from a verified customer review.
NF-H Antibody

Immunohistochemistry-Paraffin: Chicken Polyclonal NF-H Antibody [NB300-217] -

Immunohistochemistry-Paraffin: Chicken Polyclonal NF-H Antibody [NB300-217] - FFPE section of human colon showing NF-H NB300-217 immunoreactivity in enteric neurons. Primary antibody was diluted 1 in 2000 and left on tissue sections for 30m at room temperature. Secondary was donkey anti chicken HRP. Image from a verified customer review.

Applications for NF-H Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:20000

Immunohistochemistry

1:20000

Immunohistochemistry Free-Floating

1:5000

Western Blot

1:100000

Reviewed Applications

Read 8 reviews rated 4.9 using NB300-217 in the following applications:

Formulation, Preparation, and Storage

Purification

IgY purified

Formulation

Supplied as a concentrated total IgY preparation from egg yolk, dialyzed against PBS with added preservative.

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NF-H

Neurofilaments can be defined as the intermediate or 10nm filaments found specifically in neuronal cells. Neurofilaments appears as fibres of indeterminate length which generally have fine wispy protrusions from their sides. They are found abundantly in axons of large projection neurons. They probably function to provide structural support for neurons and their synapses and to support the large axon diameters required for rapid axon impulse conduction. They are composed of a mixture of subunits which usually include the three neurofilament triplet proteins, known as Neurofilament Light (NF-L), Neurofilament Medium (NF-M) and Neurofilament Heavy (NF-H). Neurofilaments may also include smaller amounts of peripherin, a-internexin, nestin and in some cases vimentin. Antibodies to the various neurofilament subunits are very useful cell type markers since the proteins are among the most abundant of the nervous system, are expressed only in neurons, and are biochemically very stable.

Long Name

Neurofilament Protein, Heavy Chain

Alternate Names

NEFH, NFH

Entrez Gene IDs

4744 (Human); 380684 (Mouse); 24587 (Rat)

Gene Symbol

NEFH

UniProt

P12036

Additional NF-H Products

Product Documents for NF-H Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for NF-H Antibody - BSA Free

Chicken products cannot be exported to Canada.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for NF-H Antibody - BSA Free

Customer Reviews for NF-H Antibody - BSA Free (8)

4.9 out of 5
8 Customer Ratings
5 Stars
88%
4 Stars
13%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 5 of 8 reviews Showing All
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  • NF-H Antibody
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Colon
    Species: Human
    Verified Customer | Posted 04/18/2024
    FFPE section of human colon showing NFH NB300-217 immunoreactivity in enteric neurons. Primary antibody was diluted 1 in 2000 and left on tissue sections for 30m at room temperature. Secondary was donkey anti chicken HRP.
    Sections were subject to heat induced epitope retrieval in Target Retrieval Solution for 20m in a vegetable steamer.
    NF-H Antibody - BSA Free NB300-217
  • NF-H Antibody
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Jejunum
    Species: Horse
    Verified Customer | Posted 04/18/2024
    FFPE section of horse jejunum showing NFH NB300-217 immunoreactivity in enteric neurons. Primary antibody was diluted 1 in 2000 and left on tissue sections for 30m at room temperature. Secondary was donkey anti chicken HRP.
    Sections were subject to heat induced epitope retrieval in Target Retrieval Solution for 20m in a vegetable steamer.
    NF-H Antibody - BSA Free NB300-217
  • NF-H Antibody
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Colon
    Species: Cat
    Verified Customer | Posted 04/18/2024
    FFPE section of cat colon showing NFH NB300-217 immunoreactivity in enteric neurons. Primary antibody was diluted 1 in 2000 and left on tissue sections for 30m at room temperature. Secondary was donkey anti chicken HRP.
    Sections were subject to heat induced epitope retrieval in Target Retrieval Solution for 20m in a vegetable steamer.
    NF-H Antibody - BSA Free NB300-217
  • NF-H Antibody
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Cecum
    Species: Dog
    Verified Customer | Posted 04/18/2024
    FFPE section of dog cecum showing NFH NB300-217 immunoreactivity in enteric neurons. Primary antibody was diluted 1 in 2000 and left on tissue sections for 30m at room temperature. Secondary was donkey anti chicken HRP.
    Sections were subject to heat induced epitope retrieval in Target Retrieval Solution for 20m in a vegetable steamer.
    NF-H Antibody - BSA Free NB300-217
  • NF-H Antibody
    Name: Anonymous
    Application: Immunohistochemistry-Frozen
    Sample Tested: Optic Nerve
    Species: Mouse
    Verified Customer | Posted 04/05/2024
    Fixed frozen section of mouse optic nerve showing neurofilament heavy NB300-217 in green and S100B in red.
    NB300-217 was diluted 1 in 4000 and was left on tissue sections overnight at room temperature. Primary was detected with donkey anti chicken conjugated to Alexa 488.
    NF-H Antibody - BSA Free NB300-217
  • NF-H Antibody
    Name: Kevin Thorburn
    Application: Immunohistochemistry-Paraffin
    Sample Tested: FFPE
    Species: Mouse
    Verified Customer | Posted 04/06/2023
    NF-H (NB300-217) immunoreactivity in a FFPE section of mouse trigeminal ganglion. Antibody was diluted 1 to 2000 and left on sections for 1h at room temperature.
    NF-H Antibody - BSA Free NB300-217
  • NF-H Antibody
    Name: Anonymous
    Application: Immunohistochemistry-Frozen
    Sample Tested: Optic nerve
    Species: Canine
    Verified Customer | Posted 02/06/2019
    NB300-217 antibody specifically labeled the axons (green). DAPI (blue).This image was taken with epifluorescent microscope.
    NB300-217 was incubated at 1:10000 for 1hr at RT.
    NF-H Antibody - BSA Free NB300-217
  • NF-H Antibody
    Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: optic nerve
    Species: Feline
    Verified Customer | Posted 05/10/2017
    Alexa Fluor 488 conjugated antibody, Primary antibody dilution 1:5000
    NF-H Antibody - BSA Free NB300-217

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FAQs for NF-H Antibody - BSA Free

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  • Q: What is the concentration of this product?

    A: For NB300-217 Lot: 27967, the concentration is 22.5mg/ml (Total IgY prep).

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