NF-M Antibody (3H11) - BSA Free
Novus Biologicals | Catalog # NB300-134
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Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Porcine, Bovine, Chicken, Equine
Cited:
Mouse, Rat
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry Free-Floating, Western Blot, Immunocytochemistry/ Immunofluorescence
Cited:
IF/IHC
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 3H11
Format
BSA Free
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Product Specifications
Immunogen
Recombinant fusion protein containing the extreme C-terminus of rat NF-M, amino acids 677-845, expressed in and purified from E. coli
Localization
Cytoplasmic
Marker
Neuronal Marker
Specificity
Specifically recognizes the medium neurofilament subunit (~145-160 kDa).
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
145-160 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for NF-M Antibody (3H11) - BSA Free
Western Blot: NF-M Antibody (3H11) [NB300-134]
Western Blot: NF-M Antibody (3H11) [NB300-134] - Neuronal tissue lysates using mouse mAb to NF-M, dilution 1:10,000 in green: [1] protein standard (red), [2] rat spinal cord, [3] mouse spinal cord, [4] cow spinal cord, [5] rat sciatic nerve. Strong bands at ~145 kDa corresponds to rodent NF-M while that at about 160 kDa corresponds to the significantly larger bovine NF-M protein.Immunohistochemistry Free-Floating: NF-M Antibody (3H11) [NB300-134]
Immunohistochemistry Free-Floating: NF-M Antibody (3H11) [NB300-134] - Adult rat frontal cortex section stained with mouse mAb to neurofilament NF-M, dilution 1:5,000 in green, and costained with chicken pAb to neurofilament NF-H, dilution 1:5,000 in red. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. NB300-134 antibody labels neuron cell bodies and dendrites of pyramidal neurons, as well as dendrites and axons of other neuronal cells, while the NF-H antibody stains the network of neuronal axons only.Western Blot: NF-M Antibody (3H11) [NB300-134]
Western Blot: 160kDa Neurofilament Medium Antibody (3H11) [NB300-134] - Rat spinal cord homogenate showing the major intermediate filament proteins of the nervous system (lane 1). The remaining lanes show blots of this material stainted with various antibodies including NB300-134 (lane 3).Applications for NF-M Antibody (3H11) - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:2000-1:5000
Immunohistochemistry
1:5000-1:10000
Immunohistochemistry Free-Floating
1:5000-1:10000
Immunohistochemistry-Paraffin
1:5000-1:10000
Western Blot
1:5000-1:10000
Application Notes
This 145-160kDa Neurofilament Medium antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections and Western blot. *The investigator should determine the optimal dilution for a specific application.
Formulation, Preparation, and Storage
Purification
Unpurified
Formulation
50% PBS, 50% glycerol
Format
BSA Free
Preservative
5mM Sodium Azide
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: NF-M
Long Name
Neurofilament Protein, Medium Chain
Alternate Names
NEF3, NEFM, NFM
Gene Symbol
NEFM
UniProt
Additional NF-M Products
Product Documents for NF-M Antibody (3H11) - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for NF-M Antibody (3H11) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for NF-M Antibody (3H11) - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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