NF-M Antibody (JM11-20)

Novus Biologicals | Catalog # NBP2-66978

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JM11-20 expressed in HEK293
View all NF-M Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide within Human NF-M aa 651-692 / 916. (SwissProt: P07197 Human; SwissProt: P08553 Mouse; SwissProt: P12839 Rat)

Localization

Cell projection, Cytoplasm, Cytoskeleton, Intermediate filament.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NF-M Antibody (JM11-20)

Western Blot: NF-M Antibody (JM11-20) [NBP2-66978]

Western Blot: NF-M Antibody (JM11-20) [NBP2-66978]

Western Blot: NF-M Antibody (JM11-20) [NBP2-66978] - Analysis of NEFM on 293T cells lysates using anti-NEFM antibody at 1/500 dilution.
Immunocytochemistry/ Immunofluorescence: NF-M Antibody (JM11-20) [NBP2-66978]

Immunocytochemistry/ Immunofluorescence: NF-M Antibody (JM11-20) [NBP2-66978]

Immunocytochemistry/Immunofluorescence: NF-M Antibody (JM11-20) [NBP2-66978] - Staining NEFM in PC-12 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: NF-M Antibody (JM11-20) [NBP2-66978]

Immunohistochemistry-Paraffin: NF-M Antibody (JM11-20) [NBP2-66978]

Immunohistochemistry-Paraffin: NF-M Antibody (JM11-20) [NBP2-66978] - Analysis of paraffin-embedded human brain tissue with Rabbit anti-NF-M antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunocytochemistry/ Immunofluorescence: NF-M Antibody (JM11-20) [NBP2-66978]

Immunocytochemistry/ Immunofluorescence: NF-M Antibody (JM11-20) [NBP2-66978]

Immunocytochemistry/Immunofluorescence: NF-M Antibody (JM11-20) [NBP2-66978] - Staining NEFM in SH-SY5Y cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunocytochemistry/ Immunofluorescence: NF-M Antibody (JM11-20) [NBP2-66978]

Immunocytochemistry/ Immunofluorescence: NF-M Antibody (JM11-20) [NBP2-66978]

Immunocytochemistry/Immunofluorescence: NF-M Antibody (JM11-20) [NBP2-66978] - Staining NEFM in N2A cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: NF-M Antibody (JM11-20) [NBP2-66978]

Immunohistochemistry-Paraffin: NF-M Antibody (JM11-20) [NBP2-66978]

Immunohistochemistry-Paraffin: NF-M Antibody (JM11-20) [NBP2-66978] - Analysis of paraffin-embedded mouse brain tissue using anti-NEFM antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: NF-M Antibody (JM11-20) [NBP2-66978]

Immunohistochemistry-Paraffin: NF-M Antibody (JM11-20) [NBP2-66978]

Immunohistochemistry-Paraffin: NF-M Antibody (JM11-20) [NBP2-66978] - Analysis of paraffin-embedded rat brain tissue using anti-NEFM antibody. Counter stained with hematoxylin.

Applications for NF-M Antibody (JM11-20)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:500

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

1:1000-1:2000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NF-M

Neurofilaments are 10nm intermediate filament proteins located in vertebrate neurons, which regulate axonal diameter. They are composed predominantly of the three major neurofilament proteins: NF-Light, NF-Medium and NF-Heavy. NF-M is the middle or medium neurofilament subunit and runs on SDS-PAGE gels in the range 145-170kDa, with some size variation in across species.

Antibodies to NF-M may be used to identify NF-M in neurons and to study neuronal processes in tissue sections and/or culture. NF-M can also be useful in studies of neurofilament accumulations seen in many neurological diseases, such as Lou Gehrig's disease or Alzheimer's disease.

Long Name

Neurofilament Protein, Medium Chain

Alternate Names

NEF3, NEFM, NFM

Gene Symbol

NEFM

Additional NF-M Products

Product Documents for NF-M Antibody (JM11-20)

Certificate of Analysis

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Product Specific Notices for NF-M Antibody (JM11-20)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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