NF-M Antibody

Novus Biologicals | Catalog # NB300-222

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Bovine, Chicken, Drosophila, Equine

Cited:

Human, Mouse, Rat, Insect - Drosophila

Applications

Validated:

Immunohistochemistry, Immunohistochemistry Free-Floating, Western Blot, Immunoblotting, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Cited:

Immunohistochemistry-Paraffin, Western Blot, Immunoblotting, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Polyclonal Chicken IgY
View all NF-M Primary Antibodies »

Product Specifications

Immunogen

Recombinant construct containing the C-terminus of the human sequence (amino acids 708-877) expressed in and purified from E. coli.

Reactivity Notes

Drosophila reactivity reported in scientific literature (PMID:31811140).

Marker

Neuronal Marker

Clonality

Polyclonal

Host

Chicken

Isotype

IgY

Theoretical MW

160 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NF-M Antibody

Western Blot: NF-M Antibody [NB300-222]

Western Blot: NF-M Antibody [NB300-222]

Western Blot: NF-M Antibody [NB300-222] - Western blot analysis of different neuronal tissue and cell lysates using chicken pAb to NF-M, NB300-222, dilution 1:2,000 in green: [1] protein standard (red), [2] rat brain [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] NIH/3T3 cells, [7] HEK293, [8] HeLa, [9] SH-SY5Y, and [10] C6 cells. Strong band at 145kDa corresponds to rodent NF-M, and about 160kDa band corresponds to human NF-M protein, visible in SHSY-5Y and HEK293 cells which have neuronal properties. NF-M is not expressed in HeLa and other cell lines tested.
Immunocytochemistry/ Immunofluorescence: NF-M Antibody [NB300-222]

Immunocytochemistry/ Immunofluorescence: NF-M Antibody [NB300-222]

Immunocytochemistry/Immunofluorescence: NF-M Antibody [NB300-222] - 160kDa Neurofilament Medium Antibody [NB300-222] - View of mixed neuron/glial cultures stained with NB300-222 (red). The NF-M protein is assembled into neurofilaments which are found throughout the axons, dendrites and perikarya of these cells.
Immunohistochemistry Free-Floating: NF-M Antibody [NB300-222]

Immunohistochemistry Free-Floating: NF-M Antibody [NB300-222]

Immunohistochemistry Free-Floating: NF-M Antibody [NB300-222] - Immunofluorescent analysis of rat cerebellum section stained with chicken pAb to NF-M, NB300-222, dilution 1:1,000 in red, and costained with mouse mAb to CNPase, dilution 1:500, in green. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free floating sections were stained with above antibodies. The NF-M antibody labels the axons of basket cells and other neurons, while the CNP antibody stains oligodendrocytes, cells that form the myelin sheathes around axons.

Applications for NF-M Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:1000

Immunohistochemistry

1:1000

Western Blot

1:10000
Application Notes
This 160kDa Neurofilament Medium antibody is useful for ICC/IF, IHC and Western blot. In Western blot this antibody detects a band at ~145-160kDa. Knockdown validation (PMID: 31811140). Use in immunoblotting reported in scientific literature (PMID:31811140).

Reviewed Applications

Read 1 review rated 5 using NB300-222 in the following applications:

Formulation, Preparation, and Storage

Purification

IgY purified

Formulation

Supplied as a concentrated total IgY preparation from egg yolk, dialyzed against PBS with added preservative.

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NF-M

Neuronal intermediate filaments (NIF) are type-IV intermediate filaments found specifically on neurons where they play key role in maintaining neuronal morphology as well as in regenerating myelinated axons. NIF contain three subunits: a light polypeptide (NEFL/NFL), a medium polypeptide (NEFM/NFM), and a heavy polypeptide (NEFH/NFH), with molecular weights of 68, 160, and 212 kD respectively. Mature NIFs are heteropolymers with about 4:2:1 stoichiometric ratio of NEFL: NEFM: NEFH and also contain a fourth type-IV intermediate filament (IF) subunit, alpha-internexin. NEFM is essential to cross-bridge formation, stabilization, and longitudinal extension of filamentous network and is more critical than NEFH in regulating NIF's structure and function. NEFM is crucial for the acquisition of normal axonal caliber in response to a myelin-dependent "outside-in" trigger for radial axonal growth and removal of the tail domain as well as lysine-serine-proline (KSP) repeats of NEFM, but not NEFH, produces axons with impaired radial growth and reduced conduction velocities. Moreover, mutations of NEFM have been associated with CNS/PNS disorders such as ALS, Parkinson's disease etc., and an imbalance in NEFM's O-GlcNAcylation - phosphorylation regulation plays a crucial role in in Alzheimer disease.

Long Name

Neurofilament Protein, Medium Chain

Alternate Names

NEF3, NEFM, NFM

Entrez Gene IDs

4741 (Human); 24588 (Rat)

Gene Symbol

NEFM

UniProt

P07197

Additional NF-M Products

Product Documents for NF-M Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for NF-M Antibody

Chicken products cannot be exported to Canada.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for NF-M Antibody (1)

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  • NF-M Antibody
    Name: Karthik Krishnamurthy
    Application: Immunocytochemistry
    Sample Tested: Cortical neurons
    Species: Rat
    Verified Customer | Posted 10/17/2017
    Neurofilament staining in cultured cortical neurons
    Confocal microscope image of cultured rat cortical (14 days invitro) neurons fixed with 4% paraformaldehyde and stained for Neurofilament (red) and MAP-2 (green). Neurofilament antibody was used at a dilution of 1:1000 and incubated at 4 degrees overnight.
    NF-M Antibody NB300-222

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Protocols

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