NF-M Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-87753

Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Validated:

Human, Mouse

Predicted:

Rat (98%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all NF-M Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: YIEKVHYLEQQNKEIEAEIQALRQKQASHAQLGDAYDQEIRELRATLEMVNHEKAQVQLDSDHLEEDIHRLKERFEEEARLRDDTEAAIRALRKDIEEASLVKVELDKKVQSLQDEVAFLRSNH

Marker

Neuronal Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NF-M Antibody - BSA Free

Immunohistochemistry-Paraffin: NF-M Antibody [NBP1-87753]

Immunohistochemistry-Paraffin: NF-M Antibody [NBP1-87753]

Immunohistochemistry-Paraffin: NF-M Antibody [NBP1-87753] - Analysis in human cerebral cortex and endometrium tissues using NBP1-87753 antibody. Corresponding NEFM RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: NF-M Antibody [NBP1-87753]

Immunohistochemistry-Paraffin: NF-M Antibody [NBP1-87753]

Immunohistochemistry-Paraffin: NF-M Antibody [NBP1-87753] - Staining of human cerebellum shows moderate cytoplasmic positivity in Purkinje cell.
Western Blot: NF-M Antibody [NBP1-87753]

Western Blot: NF-M Antibody [NBP1-87753]

Western Blot: NF-M Antibody [NBP1-87753] - Analysis NBP1-87753 (A) shows similar pattern to independent antibody NBP1-87754 (B).
Immunocytochemistry/ Immunofluorescence: NF-M Antibody [NBP1-87753]

Immunocytochemistry/ Immunofluorescence: NF-M Antibody [NBP1-87753]

Immunocytochemistry/Immunofluorescence: NF-M Antibody [NBP1-87753] - Staining of human cell line HEK 293 shows localization to intermediate filaments. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: NF-M Antibody [NBP1-87753]

Immunohistochemistry-Paraffin: NF-M Antibody [NBP1-87753]

Immunohistochemistry-Paraffin: NF-M Antibody [NBP1-87753] - Staining of human endometrium shows no positivity in glandular cells as expected.
Western Blot: NF-M Antibody [NBP1-87753]

Western Blot: NF-M Antibody [NBP1-87753]

Western Blot: NF-M Antibody [NBP1-87753] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10 Lane 2: Mouse Cerebral Cortex tissue
Western Blot: NF-M Antibody [NBP1-87753]

Western Blot: NF-M Antibody [NBP1-87753]

Western Blot: NF-M Antibody [NBP1-87753] - Analysis in human cell line HEK 293 and human cell line A-431.
Immunocytochemistry/ Immunofluorescence: NF-M Antibody [NBP1-87753]

Immunocytochemistry/ Immunofluorescence: NF-M Antibody [NBP1-87753]

Immunocytochemistry/Immunofluorescence: NF-M Antibody [NBP1-87753] - Staining of human cell line A-431 shows positivity in cytoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: NF-M Antibody [NBP1-87753]

Immunohistochemistry-Paraffin: NF-M Antibody [NBP1-87753]

Immunohistochemistry-Paraffin: NF-M Antibody [NBP1-87753] - Staining of human cerebral cortex shows strong positivity in neuronal processes.
Immunohistochemistry-Paraffin: NF-M Antibody [NBP1-87753]

Immunohistochemistry-Paraffin: NF-M Antibody [NBP1-87753]

Immunohistochemistry-Paraffin: NF-M Antibody [NBP1-87753] - Staining of human prostate shows strong positivity in peripheral nerve.
Immunohistochemistry-Paraffin: NF-M Antibody [NBP1-87753]

Immunohistochemistry-Paraffin: NF-M Antibody [NBP1-87753]

Immunohistochemistry-Paraffin: NF-M Antibody [NBP1-87753] - Staining of human tonsil shows no positivity in lymphoid cells as expected.

Applications for NF-M Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:2500 - 1:5000

Immunohistochemistry-Paraffin

1:2500 - 1:5000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval method is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NF-M

Neurofilaments are 10nm intermediate filament proteins located in vertebrate neurons, which regulate axonal diameter. They are composed predominantly of the three major neurofilament proteins: NF-Light, NF-Medium and NF-Heavy. NF-M is the middle or medium neurofilament subunit and runs on SDS-PAGE gels in the range 145-170kDa, with some size variation in across species.

Antibodies to NF-M may be used to identify NF-M in neurons and to study neuronal processes in tissue sections and/or culture. NF-M can also be useful in studies of neurofilament accumulations seen in many neurological diseases, such as Lou Gehrig's disease or Alzheimer's disease.

Long Name

Neurofilament Protein, Medium Chain

Alternate Names

NEF3, NEFM, NFM

Gene Symbol

NEFM

Additional NF-M Products

Product Documents for NF-M Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for NF-M Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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