NGFR/TNFRSF16/p75NTR Antibody

Name: NGFR/TNFRSF16/p75NTR Antibody
Brand: Novus Biologicals
SKU: NBP2-19669
Price: 479 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-19669

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Product Documents

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Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (97%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Simple Western

Cited:

Simple Western

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

View all NGFR/TNFRSF16 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human NGFR/TNFRSF16/p75NTR. The exact sequence is proprietary.

Localization

Membrane; Single-pass type I membrane protein

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NGFR/TNFRSF16/p75NTR Antibody

Immunohistochemistry-Paraffin: NGFR/TNFRSF16/p75NTR Antibody [NBP2-19669]

Immunohistochemistry-Paraffin: NGF R/TNFRSF16/p75NTR Antibody [NBP2-19669] - Rat brain. p75 NGF Receptor antibody diluted at 1:500.

Western Blot: NGFR/TNFRSF16/p75NTR Antibody [NBP2-19669] -

Western Blot: NGFR/TNFRSF16/p75NTR Antibody [NBP2-19669] - Untreated (-) and treated (+) PC-3 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with NGFR/TNFRSF16/p75NTR antibody (NBP2-19669) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry/ Immunofluorescence: NGFR/TNFRSF16/p75NTR Antibody [NBP2-19669] -

p75 NGF Receptor / CD271 antibody detects p75 NGF Receptor / CD271 protein by immunofluorescent analysis.
Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: p75 NGF Receptor / CD271 stained by p75 NGF Receptor / CD271 antibody (NBP2-19669) diluted at 1:500.
Red: Tau, stained by Tau antibody [GT287] diluted at 1:500.
Blue: Fluoroshield with DAPI.

Western Blot: NGFR/TNFRSF16/p75NTR Antibody [NBP2-19669] -

Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with p75 NGF Receptor / CD271 antibody (NBP2-19669) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunohistochemistry-Frozen: NGFR/TNFRSF16/p75NTR Antibody [NBP2-19669] -

p75 NGF Receptor antibody detects p75 NGF Receptor protein expression by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: p75 NGF Receptor protein stained by p75 NGF Receptor antibody (NBP2-19669) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:500.
Blue: Fluoroshield with DAPI.

Western Blot: NGFR/TNFRSF16/p75NTR Antibody [NBP2-19669] -

p75 NGF Receptor antibody detects p75 NGF Receptor protein by western blot analysis. Whole cell extracts (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with p75 NGF Receptor antibody (NBP2-19669) at a dilution of 1:2000.

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Applications for NGFR/TNFRSF16/p75NTR Antibody

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Frozen

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Simple Western

1:100

Western Blot

1:500-1:3000

Application Notes

Flow-Assay dependent (Membrane permeabilization is required for this application).
See Simple Western Antibody Database for Simple Western validation: Tested in Skin, separated by Size, antibody dilution of 1:100

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Advanced Features

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Antigen Density Selector - Match fluorochrome brightness with antigen density

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Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: NGFR/TNFRSF16

Nerve growth factor receptor contains an extracellular domain containing four 40-amino acid repeats with 6 cysteine residues at conserved positions followed by a serine/threonine-rich region, a single transmembrane domain, and a 155-amino acid cytoplasmic domain. The cysteine-rich region contains the nerve growth factor binding domain. [provided by RefSeq]

Long Name

Nerve Growth Factor Receptor

Alternate Names

CD271, NGF R, p75 NTR, TNFRSF16

Gene Symbol

NGFR

UniProt

P08138

Additional NGFR/TNFRSF16 Products

Product Documents for NGFR/TNFRSF16/p75NTR Antibody

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Certificate of Analysis

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Product Specific Notices for NGFR/TNFRSF16/p75NTR Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.