NHE1/SLC9A1 Antibody - BSA Free
Novus Biologicals | Catalog # NBP1-76847
Key Product Details
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Label
Antibody Source
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Product Specifications
Immunogen
Specificity
Clonality
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Theoretical MW
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for NHE1/SLC9A1 Antibody - BSA Free
Western Blot: NHE1/SLC9A1 AntibodyBSA Free [NBP1-76847]
Western Blot: NHE1/SLC9A1 Antibody [NBP1-76847] - Rat kidney tissue lysate with in with Nhe-1 antibody at (A) 1 and (B) 2 ug/ml.Immunohistochemistry: NHE1/SLC9A1 Antibody - BSA Free [NBP1-76847] -
Immunohistochemistry: NHE1/SLC9A1 Antibody - BSA Free [NBP1-76847] - Immunohistochemical staining of human brain tissue using NHE1/SLC9A1 antibody at 2.5 ug/mL.Immunocytochemistry/ Immunofluorescence: NHE1/SLC9A1 Antibody - BSA Free [NBP1-76847] -
Immunocytochemistry/ Immunofluorescence: NHE1/SLC9A1 Antibody - BSA Free [NBP1-76847] - Immunofluorescence of NHE1/SLC9A1 in Human Brain tissue with NHE1/SLC9A1 antibody at 20 ug/mL.Western Blot: NHE1/SLC9A1 Antibody - BSA Free [NBP1-76847] -
Activity and protein expression of NHE1 between WT and db/db after HTK-induced arrest. (a) NHE activity was assessed by NH4Cl pulse technique in LV cardiac strips of WT (closed black square) and db/db (closed red square) mice following cardioplegia-induced arrest. Full image of NHE activity was represented in the square box, and magnified images were obtained from the dotted line. (b) NHE activity in LV of WT after cardioplegia-induced arrest. (c) Analysis of NHE activity. The slope of pHi was measured in NHE activity in the Na+-containing Regular solution. The bars represent the mean +/- SEM (n = 3). (d) Expression of NHE1 protein in LV and RV cardiac strips of WT and db/db following cardioplegia-induced arrest. (e) Analysis of band intensity of NHE1 in LV. The beta -actin was used as the loading control. The bars show the mean +/- SEM (n = 3). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31582903), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for NHE1/SLC9A1 Antibody - BSA Free
ELISA
Immunocytochemistry/ Immunofluorescence
Immunohistochemistry
Immunohistochemistry-Paraffin
Western Blot
Formulation, Preparation, and Storage
Purification
Formulation
Format
Preservative
Concentration
Shipping
Stability & Storage
Background: NHE1/SLC9A1
Long Name
Alternate Names
Entrez Gene IDs
Gene Symbol
UniProt
Additional NHE1/SLC9A1 Products
Product Documents for NHE1/SLC9A1 Antibody - BSA Free
Certificate of Analysis
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Product Specific Notices for NHE1/SLC9A1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for NHE1/SLC9A1 Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for NHE1/SLC9A1 Antibody - BSA Free
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Q: I would like to know if the NHE1 antibody binds to intracellular compartments that may have NHE1 (I.e. the mitochondria) or if they only bind to the plasma membrane NHE1
A:
The NHE1 Antibody (NBP1-76847) was raised against a 20 amino acid peptide near the carboxyl terminus of the human Nhe-1 protein. From uniprot we can see in subcelluar location that AAs 500-815 correspond to the topological domain for the cytoplasmic region where this epitope should be located however we do not know of any specific interaction with organelles within the cytoplasm such as the mitochondria however uniprot does mention the Endoplasmic Reticulum membrane. http://www.uniprot.org/uniprot/P19634#subcellular_location