NHE3/SLC9A3 Antibody - BSA Free
Novus Biologicals | Catalog # NBP1-82575
![Immunocytochemistry/ Immunofluorescence: NHE3/SLC9A3 Antibody [NBP1-82575]](https://resources.rndsystems.com/images/products/NHE3-SLC9A3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-82575-img0015.jpg "Immunocytochemistry/ Immunofluorescence: NHE3/SLC9A3 Antibody [NBP1-82575]")
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Scientific Data Images for NHE3/SLC9A3 Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence: NHE3/SLC9A3 Antibody [NBP1-82575]
Immunocytochemistry/Immunofluorescence: NHE3/SLC9A3 Antibody [NBP1-82575] - Immunofluorescence micrograph of human intestine showing polarized distribution of apical (NHE3) protein. ICC/IF image submitted by a verified customer review.![Immunocytochemistry/ Immunofluorescence: NHE3/SLC9A3 Antibody [NBP1-82575]](https://resources.rndsystems.com/images/products/NHE3-SLC9A3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-82575-img0010.jpg "Immunocytochemistry/ Immunofluorescence: NHE3/SLC9A3 Antibody [NBP1-82575]")
Immunocytochemistry/ Immunofluorescence: NHE3/SLC9A3 Antibody [NBP1-82575]
Immunocytochemistry/Immunofluorescence: NHE3/SLC9A3 Antibody [NBP1-82575] - Staining of human cell line U-2 OS shows localization to plasma membrane. Antibody staining is shown in green.Applications for NHE3/SLC9A3 Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence
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Read 1 review rated 5 using NBP1-82575 in the following applications:
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Background: NHE3/SLC9A3
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Product Documents for NHE3/SLC9A3 Antibody - BSA Free
Certificate of Analysis
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Product Specific Notices for NHE3/SLC9A3 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Application: ImmunocytochemistrySample Tested: Small intestineSpecies: Human small intestine and HumanVerified Customer | Posted 03/25/2020Immunofluorescence micrographs of the Human Intestine showing polarized distribution of apical (NHE3) protein.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for NHE3/SLC9A3 Antibody - BSA Free
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Q: The molecular weight of NHE3 is 92kDa but I see a band above 100 kDa. I am confused, because the size of these protein should not be greater than 100KD. Can you please help explain?
A: Higher than predicted molecular weight seen on WB is not uncommon if the protein is posttranslationally modified, especially glycosylated, and such is the case for NHE3. Please note that NHE3 gets phosphorylated and glycosylated (Uniprot ID P48764) and potentially, these modifications are adding ~10kDa extra to the predicted molecular weight. If you want to confirm it 100%, you could try de-glycosylating your protein and then performing a WB on it.