NHE3/SLC9A3 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-82575

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Mouse (93%), Rat (93%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all NHE3/SLC9A3 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: TDNVVNVDFTPRSSTVEASVSYLLRENVSAVCLDMQSLEQRRRSIRDAEDMVTHHTLQQYLYKPRQEYKHLYSRHELTPTEDEKQDREIFHRTM

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NHE3/SLC9A3 Antibody - BSA Free

Immunohistochemistry-Paraffin: NHE3/SLC9A3 Antibody [NBP1-82575]

Immunohistochemistry-Paraffin: NHE3/SLC9A3 Antibody [NBP1-82575]

Immunohistochemistry-Paraffin: NHE3/SLC9A3 Antibody [NBP1-82575] - Staining of human cerebral cortex, colon, gallbladder and lymph node using Anti-SLC9A3 antibody NBP1-82575 (A) shows similar protein distribution across tissues to independent antibody NBP1-82574 (B).
Immunocytochemistry/ Immunofluorescence: NHE3/SLC9A3 Antibody [NBP1-82575]

Immunocytochemistry/ Immunofluorescence: NHE3/SLC9A3 Antibody [NBP1-82575]

Immunocytochemistry/Immunofluorescence: NHE3/SLC9A3 Antibody [NBP1-82575] - Immunofluorescence micrograph of human intestine showing polarized distribution of apical (NHE3) protein. ICC/IF image submitted by a verified customer review.
Immunohistochemistry-Paraffin: NHE3/SLC9A3 Antibody [NBP1-82575]

Immunohistochemistry-Paraffin: NHE3/SLC9A3 Antibody [NBP1-82575]

Immunohistochemistry-Paraffin: NHE3/SLC9A3 Antibody [NBP1-82575] - Staining of human liver shows no membranous positivity in hepatocytes as expected.
Immunocytochemistry/ Immunofluorescence: NHE3/SLC9A3 Antibody [NBP1-82575]

Immunocytochemistry/ Immunofluorescence: NHE3/SLC9A3 Antibody [NBP1-82575]

Immunocytochemistry/Immunofluorescence: NHE3/SLC9A3 Antibody [NBP1-82575] - Staining of human cell line U-2 OS shows localization to plasma membrane. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: NHE3/SLC9A3 Antibody [NBP1-82575]

Immunohistochemistry-Paraffin: NHE3/SLC9A3 Antibody [NBP1-82575]

Immunohistochemistry-Paraffin: NHE3/SLC9A3 Antibody [NBP1-82575] - Analysis in human colon and liver tissues using NBP1-82575 antibody. Corresponding SLC9A3 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: NHE3/SLC9A3 Antibody [NBP1-82575]

Immunohistochemistry-Paraffin: NHE3/SLC9A3 Antibody [NBP1-82575]

Immunohistochemistry-Paraffin: NHE3/SLC9A3 Antibody [NBP1-82575] - Staining of human colon shows strong positivity in apical membrane in glandular cells.
Immunohistochemistry-Paraffin: NHE3/SLC9A3 Antibody [NBP1-82575]

Immunohistochemistry-Paraffin: NHE3/SLC9A3 Antibody [NBP1-82575]

Immunohistochemistry-Paraffin: NHE3/SLC9A3 Antibody [NBP1-82575] - Staining of human gallbladder shows strong positivity in apical membrane in glandular cells.
Immunohistochemistry-Paraffin: NHE3/SLC9A3 Antibody [NBP1-82575]

Immunohistochemistry-Paraffin: NHE3/SLC9A3 Antibody [NBP1-82575]

Immunohistochemistry-Paraffin: NHE3/SLC9A3 Antibody [NBP1-82575] - Staining of human kidney shows moderate positivity in apical membrane in cells in tubules.
Immunohistochemistry-Paraffin: NHE3/SLC9A3 Antibody [NBP1-82575]

Immunohistochemistry-Paraffin: NHE3/SLC9A3 Antibody [NBP1-82575]

Immunohistochemistry-Paraffin: NHE3/SLC9A3 Antibody [NBP1-82575] - Staining of human small intestine shows moderate positivity in apical membrane in glandular cells.

Applications for NHE3/SLC9A3 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Reviewed Applications

Read 1 review rated 5 using NBP1-82575 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NHE3/SLC9A3

NHE3 is a member of the Sodium/Hydrogen exchanger family that plays a crucial role in acid-base and volume homeostasis by mediating the majority of sodium and bicarbonate reabsorption in the proximal tubule of the kidney. Two PKA consensus sites have been discovered in rat NHE3, serine 552 and serine 605. Research suggests that dopamine-induced phosphorylation of these consensus sites inhibit NHE3.

Long Name

Sodium/Hydrogen Exchanger 3

Alternate Names

SLC9A3

Gene Symbol

SLC9A3

Additional NHE3/SLC9A3 Products

Product Documents for NHE3/SLC9A3 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for NHE3/SLC9A3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Ion Channels and Regulators

Citations for NHE3/SLC9A3 Antibody - BSA Free

Customer Reviews for NHE3/SLC9A3 Antibody - BSA Free (1)

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Customer Images

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  • NHE3/SLC9A3 Antibody
    Name: Sasan Jalili
    Application: Immunocytochemistry
    Sample Tested: Small intestine
    Species: Human small intestine and Human
    Verified Customer | Posted 03/25/2020
    Immunofluorescence micrographs of the Human Intestine showing polarized distribution of apical (NHE3) protein.
    NHE3/SLC9A3 Antibody - BSA Free NBP1-82575

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Protocols

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FAQs for NHE3/SLC9A3 Antibody - BSA Free

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  • Q: The molecular weight of NHE3 is 92kDa but I see a band above 100 kDa.  I am confused, because the size of these protein should not be greater than 100KD. Can you please help explain?  

    A: Higher than predicted molecular weight seen on WB is not uncommon if the protein is posttranslationally modified, especially glycosylated, and such is the case for NHE3. Please note that NHE3 gets phosphorylated and glycosylated (Uniprot ID P48764) and potentially, these modifications are adding ~10kDa extra to the predicted molecular weight. If you want to confirm it 100%, you could try de-glycosylating your protein and then performing a WB on it. 

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