Nicastrin Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-77269

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Nicastrin Primary Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 17 amino acid synthetic peptide from near the carboxy terminus of human Nicastrin. The immunogen is located within amino acids 630 - 680 of Nicastrin. Amino Acid Squence: TEYSTWTESRWKDIRAR

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Nicastrin Antibody - BSA Free

Western Blot: Nicastrin AntibodyBSA Free [NBP1-77269]

Western Blot: Nicastrin AntibodyBSA Free [NBP1-77269]

Western Blot: Nicastrin Antibody [NBP1-77269] - Mouse brain tissue lysate with Nicastrin antibody at (A) 0.5, (B) 1, and (C) 2 ug/ml.
Immunocytochemistry/ Immunofluorescence: Nicastrin Antibody - BSA Free [NBP1-77269]

Immunocytochemistry/ Immunofluorescence: Nicastrin Antibody - BSA Free [NBP1-77269]

Nicastrin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77269-img0004.jpg
Immunohistochemistry-Paraffin: Nicastrin Antibody - BSA Free [NBP1-77269]

Immunohistochemistry-Paraffin: Nicastrin Antibody - BSA Free [NBP1-77269]

Immunohistochemistry-Paraffin: Nicastrin Antibody [NBP1-77269] - Human brain tissue with Nicastrin antibody at 5 ug/ml.
Nicastrin Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Nicastrin Antibody - BSA Free [NBP1-77269] -

Immunocytochemistry/ Immunofluorescence: Nicastrin Antibody - BSA Free [NBP1-77269] - Immunofluorescence of Nicastrin in Human Brain cells with Nicastrin antibody at 20 ug/mL.
Nicastrin Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Nicastrin Antibody - BSA Free [NBP1-77269] -

Cell surface staining of VDR (green) on live neurons followed by fixation and immunofluorescence labeling of target proteins (100x): A) ADAM10; B) APP; C) BACE1; D) Nicastrin; E) Presenilin 1; F) Presenilin 2. Overlay images indicate the possible co-localization of VDR/ADAM10 (A) or VDR/APP (B) or VDR/Nicastrin (D) or limited co-localization of VDR/ Presenilin 1 (E) on the neuronal plasma membrane. Overlay images do not indicate co-localization for VDR/BACE1 (C) or VDR/Presenilin 2 (F) on the neuronal plasma membrane. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/29176823), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Nicastrin Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

20 ug/ml

Immunohistochemistry

5 ug/ml

Immunohistochemistry-Paraffin

5 ug/ml

Western Blot

0.5-2 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Nicastrin

Nicastrin, in addition to presenilin, PEN2, and APH-1 forms the gamma-secretase protein complex, a membrane-bound aspartyl protease that can cleave certain proteins at peptide bonds buried within the hydrophobic environment of the lipid bilayer. This cleavage is responsible for a key step in signaling from several cell-surface receptors and is thought to be required for the generation of the neurotoxic amyloid peptides that are central to the pathogenesis of Alzheimer's disease. Like the tumor necrosis factor-a-converting enzyme (TACE) and the b-site cleavage enzyme (BACE) protease families, gamma-secretase will cleave the amyloid precursor protein (APP), but within the intramembrane region of APP, resulting in either the non-toxic p3 (from the alpha and gamma cleavage site) or the toxic Abeta amyloid peptide (from the beta and gamma cleavage site). It is thought that accumulation of the Abeta peptide is the precursor to Alzheimer's disease. Nicastrin is also thought to be involved in cell proliferation and signaling, especially in regards to activation of Notch receptors as loss of Nicastrin expression results in mouse embryonic lethality.

Alternate Names

APH2, NCSTN

Gene Symbol

NCSTN

UniProt

NP_056146 (Human)

Additional Nicastrin Products

Product Documents for Nicastrin Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Nicastrin Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Nicastrin Antibody - BSA Free

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Protocols

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