Niemann-Pick C1 Antibody - BSA Free
Novus Biologicals | Catalog # NB400-148
![Western Blot: Niemann-Pick C1 AntibodyBSA Free [NB400-148]](https://resources.rndsystems.com/images/products/Niemann-Pick-C1-Antibody---BSA-Free-Western-Blot-NB400-148-img0015.jpg "Western Blot: Niemann-Pick C1 AntibodyBSA Free [NB400-148]")
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Scientific Data Images for Niemann-Pick C1 Antibody - BSA Free
Western Blot: Niemann-Pick C1 AntibodyBSA Free [NB400-148]
Niemann-Pick-C1-Antibody---BSA-Free-Western-Blot-NB400-148-img0015.jpg![Immunocytochemistry/ Immunofluorescence: Niemann-Pick C1 Antibody - BSA Free [NB400-148]](https://resources.rndsystems.com/images/products/Niemann-Pick-C1-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NB400-148-img0010.jpg "Immunocytochemistry/ Immunofluorescence: Niemann-Pick C1 Antibody - BSA Free [NB400-148]")
Immunocytochemistry/ Immunofluorescence: Niemann-Pick C1 Antibody - BSA Free [NB400-148]
Immunocytochemistry/Immunofluorescence: Niemann-Pick C1 Antibody - BSA Free [NB400-148] - NPC1 antibody was tested in HeLa cells with DyLight 488 (green). Nuclei were counterstained with DAPI (blue).![Immunohistochemistry-Paraffin: Niemann-Pick C1 Antibody - BSA Free [NB400-148]](https://resources.rndsystems.com/images/products/Niemann-Pick-C1-Antibody---BSA-Free-Immunohistochemistry-Paraffin-NB400-148-img0011.jpg "Immunohistochemistry-Paraffin: Niemann-Pick C1 Antibody - BSA Free [NB400-148]")
Immunohistochemistry-Paraffin: Niemann-Pick C1 Antibody - BSA Free [NB400-148]
Immunohistochemistry-Paraffin: Niemann-Pick C1 Antibody - BSA Free [NB400-148] - Staining of human brain, cortex, neurons and astrocytes.![Western Blot: Niemann-Pick C1 AntibodyBSA Free [NB400-148]](https://resources.rndsystems.com/images/products/Niemann-Pick-C1-Antibody---BSA-Free-Western-Blot-NB400-148-img0016.jpg "Western Blot: Niemann-Pick C1 AntibodyBSA Free [NB400-148]")
Western Blot: Niemann-Pick C1 AntibodyBSA Free [NB400-148]
Niemann-Pick-C1-Antibody---BSA-Free-Western-Blot-NB400-148-img0016.jpg![Immunocytochemistry/ Immunofluorescence: Niemann-Pick C1 Antibody - BSA Free [NB400-148]](https://resources.rndsystems.com/images/products/Niemann-Pick%20C1%20Antibody%20-%20BSA%20Free-Immunocytochemistry-Immunofluorescence-NB400-148-img0017.jpg "Immunocytochemistry/ Immunofluorescence: Niemann-Pick C1 Antibody - BSA Free [NB400-148]")
![Knockout Validated: Niemann-Pick C1 Antibody - BSA Free [NB400-148]](https://resources.rndsystems.com/images/products/Niemann-Pick-C1-Antibody---BSA-Free-Knockout-Validated-NB400-148-img0014.jpg "Western Blot: Niemann-Pick C1 Antibody - BSA Free [NB400-148]")
Western Blot: Niemann-Pick C1 Antibody - BSA Free [NB400-148]
Western Blot: Niemann-Pick C1 Antibody - BSA Free [NB400-148] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and NPC1 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:1000 of Rabbit Anti-Human NPC1 Polyclonal Antibody (Catalog # NB400-148) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for NPC1 at approximately 240-260 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.![Knockdown Validated: Niemann-Pick C1 Antibody - BSA Free [NB400-148]](https://resources.rndsystems.com/images/products/Niemann-Pick-C1-Antibody---BSA-Free-Knockdown-Validated-NB400-148-img0012.jpg "Western Blot: Niemann-Pick C1 Antibody - BSA Free [NB400-148]")
Western Blot: Niemann-Pick C1 Antibody - BSA Free [NB400-148] -
NPC proteins in mouse lungs.Western blot of wild type (W) littermates, NPC1 (Mut) or NPC2 (Mut) mutant mouse lungs using anti-NPC1 or -NPC2 antibody. beta -actin used as a loading control. 30 µg protein/lane.
Immunocytochemistry/ Immunofluorescence: Niemann-Pick C1 Antibody - BSA Free [NB400-148] -
Immunocytochemistry/ Immunofluorescence: Niemann-Pick C1 Antibody - BSA Free [NB400-148] - ESCRT complex recruited to LLOMe-damaged lysosomes promotes cell survival.Human fibroblasts were exposed to 1 mM LLOMe & when indicated, the cell permeable calcium chelator BAPTA-AM (1 µM) was added 10 min prior to LLOMe exposure. Immunocytochemical staining with corresponding colocalization analysis of a CHMP4B (green) & LAMP2 (red), b ALIX (green) & NPC-1 (red), & c CHMP4B (green) & LAMP2 (red) in fibroblasts exposed to LLOMe for 15 min. Nuclei are stained with DAPI (blue) & merged images show colocalization in yellow (n = 5). d Caspase-3 like activity in cells exposed to LLOMe for 8 h (n = 3). e Quantification of apoptotic & necrotic cells using Annexin V/PI staining in fibroblasts exposed to LLOMe for 6 h (n = 3). f Immunoblot of LAMP2 in digitonin-extracted cytosolic fractions from cells exposed to LLOMe for 2 h (n = 8) with corresponding quantitative analysis. *p < 0.05, **p < 0.01 & ***p < 0.001 compared to control (a–b) or LLOMe only (c–f). Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32409651), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Western Blot: Niemann-Pick C1 Antibody - BSA Free [NB400-148] -
Western Blot: Niemann-Pick C1 Antibody - BSA Free [NB400-148] - Expression of proteins involved in extracellular lipid uptake & intracellular cholesterol trafficking following JQ1 (0.4 µM) administration to HepG2 cells for 48 hours. (A) Representative Western blot (left panel) & densitometric analysis (right panel) of SR-B1. n = 6 independent experiments. (B) Immunofluorescence analysis of SR-B1 (green). Nuclei were counterstained with DAPI. n = 3 different experiments. Scale bar: 50 µm. (C) Representative Western blot (left panel) & densitometric analysis (right panel) of LDLr. n = 5 independent experiments. (D) LDLr immunofluorescence (green). Nuclei were counterstained with DAPI. n = 3 different experiments. (E–F) Representative Western blots & densitometric analysis of NPC1 & TMEM97. Tubulin was chosen as loading control. n = 6 independent experiments. Data represent means ± SD. Statistical analysis was performed by using unpaired Student’s t test. ** p < 0.01; *** p < 0.001. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32075110), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Western Blot: Niemann-Pick C1 Antibody - BSA Free [NB400-148] -
Western Blot: Niemann-Pick C1 Antibody - BSA Free [NB400-148] - Effects of p75NTR modulation by LM11A-31 on cholesterol metabolism & secretion. (A–D) Representative Western blot & densitometric analysis of HMGCR, ABCA1, NPC1, & ApoE in U373 cells treated with vehicle (Ctrl) & LM11A-31 (0.1 µM) for 48 h. n = 4 different experiments. Vinculin served as a housekeeping protein to normalize protein loading. (E) Quantification of ApoE amount (µg/mL) by ELISA assay in culture medium from vehicle- & LM11A-31-treated U373 cells. n = 3 different experiments. (F) Cholesterol quantification (total cholesterol, free cholesterol, & cholesteryl esters) in the culture medium of U373 cells treated with vehicle (Ctrl) & LM11A-31 (0.1 µM) for 48 h. n = 3 different experiments. Data represent means ± SD. Statistical analysis was carried out by using unpaired Student’s t test. *** p < 0.001. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/35563230), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for Niemann-Pick C1 Antibody - BSA Free
Electron Microscopy
Immunocytochemistry/ Immunofluorescence
Immunohistochemistry
Immunohistochemistry-Paraffin
Immunoprecipitation
Western Blot
Reviewed Applications
Read 6 reviews rated 4.2 using NB400-148 in the following applications:
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Background: Niemann-Pick Type C1/NPC1
Alternate Names
Gene Symbol
UniProt
Additional Niemann-Pick Type C1/NPC1 Products
Product Documents for Niemann-Pick C1 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Niemann-Pick C1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for Niemann-Pick C1 Antibody - BSA Free
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Customer Images
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Application: ImmunofluorescenceSample Tested: hela cellSpecies: HumanVerified Customer | Posted 10/23/2019
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Application: ImmunocytochemistrySample Tested: Human fibroblastSpecies: HumanVerified Customer | Posted 11/27/2018Fixation: 3% PFA, room temp, 15 min Permeabilization: 0.05% digitonin, room temp, 10 min Blocking: 0.1% gelatin PBS, room temp, 30 min 1st Ab: Rabbit anti-NPC1 Ab (NB400-148, Lot M-2) 1:100 0.1% gelatin PBS,room temp, 1 hour 2nd Ab: Alexa488 Goat anti-rabbit IgG Ab, 1:100 0.1% gelatin PBS, room temp, 1 hour
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Application: Western BlotSample Tested: Hela whole cell lysateSpecies: HumanVerified Customer | Posted 11/01/2018
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Application: ImmunoprecipitationSample Tested: See PMID 24209575Species: HumanVerified Customer | Posted 12/23/2014
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Application: Western BlotSample Tested: See PMID 22607065Species: HumanVerified Customer | Posted 12/23/2014
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Application: Western BlotSample Tested: Hela lysate, BHK lysate, A431 lysateSpecies: HumanVerified Customer | Posted 02/28/2014Application: Western Blot, Sample Tested:Hela lysate, BHK lysate, A431 lysate, Catalog Num:NB400-148
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Protocols
View specific protocols for Niemann-Pick C1 Antibody - BSA Free (NB400-148):
IHC-FFPE sections
I. Deparaffinization:
A. Treat slides with Xylene: 3 changes for 5 minutes each. Drain slides for 10 seconds between changes.
B. Treat slides with 100% Reagent Alcohol: 3 changes for 5 minutes each. Drain slides for 10 seconds between changes.
II. Quench Endogenous Peroxidase:
A. Place slides in peroxidase quenching solution: 15-30 minutes. To Prepare 200 ml of Quenching Solution: Add 3 ml of 30% Hydrogen Peroxide to 200 ml of Methanol.
Use within 4 hours of preparation
B. Place slides in distilled water: 2 changes for 2 minutes each.
III. Retrieve Epitopes:
A. Preheat Citrate Buffer. Place 200 ml of Citrate Buffer Working Solution into container, cover and place into steamer. Heat to 90-96 degrees Celsius.
B. Place rack of slides into hot Citrate Buffer for 20 minutes. Cover.
C. Carefully remove container with slides from steamer and cool on bench, uncovered, for 20 minutes.
D. Slowly add distilled water to further cool for 5 minutes.
E. Rinse slides with distilled water. 2 changes for 2 minutes each.
IV. Immunostaining Procedure:
A. Remove each slide from rack and circle tissue section with a hydrophobic barrier pen (e.g. Liquid Blocker-Super Pap Pen).
B. Flood slide with Wash Solution. Do not allow tissue sections to dry for the rest of the procedure.
C. Drain wash solution and apply 4 drops of Blocking Reagent to each slide and incubate for 15 minutes.
D. Drain Blocking Reagent (do not wash off the Blocking Reagent), apply 200 ul of Primary Antibody solution to each slide, and incubate for 1 hour.
E. Wash slides with Wash Solution: 3 changes for 5 minutes each.
F. Drain wash solution, apply 4 drops of Secondary antibody to each slide and incubate for 1 hour.
G. Wash slides with Wash Solution: 3 changes for 5 minutes each.
H. Drain wash solution, apply 4 drops of DAB Substrate to each slide and develop for 5-10 minutes. Check development with microscope.
I. Wash slides with Wash Solution: 3 changes for 5 minutes each.
J. Drain wash solution, apply 4 drops of Hematoxylin to each slide and stain for 1-3 minutes. Increase time if darker counterstaining is desired.
K. Wash slides with Wash Solution: 2-3 changes for 2 minutes each.
L. Drain wash solution and apply 4 drops of Bluing Solution to each slide for 1-2 minutes.
M. Rinse slides in distilled water.
N. Soak slides in 70% reagent alcohol: 3 minutes with intermittent agitation.
O. Soak slides in 95% reagent alcohol: 2 changes for 3 minutes each with intermittent agitation.
P. Soak slides in 100% reagent alcohol: 3 changes for 3 minutes each with intermittent agitation. Drain slides for 10 seconds between each change.
Q. Soak slides in Xylene: 3 changes for 3 minutes each with intermittent agitation. Drain slides for 10 seconds between each change.
R. Apply 2-3 drops of non-aqueous mounting media to each slide and mount coverslip.
S. Lay slides on a flat surface to dry prior to viewing under microscope.
NOTES:
-Use treated slides (e.g. HistoBond) to assure adherence of FFPE sections to slide.
-Prior to deparaffinization, heat slides overnight in a 60 degrees Celsius oven.
-All steps in which Xylene is used should be performed in a fume hood.
-For Epitope Retrieval, a microwave or pressure cooker may be substituted for the steamer method. Adjust times as necessary depending on conditions.
-For the initial IHC run with a new primary antibody, test tissues with and without Epitope Retrieval. In some instances, Epitope Retrieval may not be necessary.
-200 ul is the recommended maximum volume to apply to a slide for full coverage. Using more than 200 ul may allow solutions to wick off the slide and create drying artifacts. For small tissue sections less than 200 ul may be used.
-5 minutes of development with DAB Substrate should be sufficient. Do not develop for more than 10 minutes. If 5 minutes of development causes background staining, further dilution of the primary antibody may be necessary.
-Hematoxylin should produce a light nuclear counterstain so as not to obscure the DAB staining. Counterstain for 1-1.5 minutes for nuclear antigens. Counterstain for 2-3 minutes for cytoplasmic and membranous antigens. If darker counterstaining is desired increase time (up to 10 minutes).
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for Niemann-Pick C1 Antibody - BSA Free
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Q: Have you received any complaints on these lots F1 and G1 of NB400-148?
A: I have looked through our complaints and spoke to our lab manager, and we have not had any recent reported problems with these lots. These lots do happen to be approximately two years old, so it may be that they have lost reactivity over this time. Our antibody products are guaranteed for 6 months from date of receipt.
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Q: I want to purchase NPC1 antibody from your company and the catalog is NB400-148. Could you please tell me in which precise position the antibody binds of the NPC1 protein?
A: NB400-148 binds between amino acids 1178 and 1278 of human Niemann-Pick C.
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Q: I'm looking for a Niemann-Pick type1 or 2 antibody to stain mouse heart section embedded in paraffin. I would like to label these sections with another primary antibody to show colocalisation of my protein of interest. I would like to know if you have a rabbit anti-Niemann-Pick antibody that works well for fluorescence immunohistochemistry?
A: Niemann-Pick C1 Antibody (NB400-148) (0.1 ml) is one of our best sellers and this antibody has been cited in at least 17 peer reviewed research publications in journals of high repute. We have only one Niemann-Pick type C2 antibody with catalog # H00010577-D01P, but this antibody is yet to be established for IHC application. However, if you would like to try this antibody for IHC-P application you would qualify for our Innovators Reward program. Through this program if you complete an online review with image, detailing your positive or negative results we will send you a discount voucher for 100% of the purchase price of the reviewed product. Read more about Innovators Reward Program.
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Q: We have an inquiry of your product Cat. ID # NB400-148. Our customer would like to know the immunogen sequence of this product. If you cannot provide the detail, would you please check if this antibody covers amino acids 600-800?
A: This antibody was made to a sequence found within amino acids 1230-1278.
-
Q: Have you received any complaints on these lots F1 and G1 of NB400-148?
A: I have looked through our complaints and spoke to our lab manager, and we have not had any recent reported problems with these lots. These lots do happen to be approximately two years old, so it may be that they have lost reactivity over this time. Our antibody products are guaranteed for 6 months from date of receipt.
-
Q: I want to purchase NPC1 antibody from your company and the catalog is NB400-148. Could you please tell me in which precise position the antibody binds of the NPC1 protein?
A: NB400-148 binds between amino acids 1178 and 1278 of human Niemann-Pick C.
-
Q: I'm looking for a Niemann-Pick type1 or 2 antibody to stain mouse heart section embedded in paraffin. I would like to label these sections with another primary antibody to show colocalisation of my protein of interest. I would like to know if you have a rabbit anti-Niemann-Pick antibody that works well for fluorescence immunohistochemistry?
A: Niemann-Pick C1 Antibody (NB400-148) (0.1 ml) is one of our best sellers and this antibody has been cited in at least 17 peer reviewed research publications in journals of high repute. We have only one Niemann-Pick type C2 antibody with catalog # H00010577-D01P, but this antibody is yet to be established for IHC application. However, if you would like to try this antibody for IHC-P application you would qualify for our Innovators Reward program. Through this program if you complete an online review with image, detailing your positive or negative results we will send you a discount voucher for 100% of the purchase price of the reviewed product. Read more about Innovators Reward Program.
-
Q: We have an inquiry of your product Cat. ID # NB400-148. Our customer would like to know the immunogen sequence of this product. If you cannot provide the detail, would you please check if this antibody covers amino acids 600-800?
A: This antibody was made to a sequence found within amino acids 1230-1278.
-
Q: Have you received any complaints on these lots F1 and G1 of NB400-148?
A: I have looked through our complaints and spoke to our lab manager, and we have not had any recent reported problems with these lots. These lots do happen to be approximately two years old, so it may be that they have lost reactivity over this time. Our antibody products are guaranteed for 6 months from date of receipt.
-
Q: I want to purchase NPC1 antibody from your company and the catalog is NB400-148. Could you please tell me in which precise position the antibody binds of the NPC1 protein?
A: NB400-148 binds between amino acids 1178 and 1278 of human Niemann-Pick C.
-
Q: I'm looking for a Niemann-Pick type1 or 2 antibody to stain mouse heart section embedded in paraffin. I would like to label these sections with another primary antibody to show colocalisation of my protein of interest. I would like to know if you have a rabbit anti-Niemann-Pick antibody that works well for fluorescence immunohistochemistry?
A: Niemann-Pick C1 Antibody (NB400-148) (0.1 ml) is one of our best sellers and this antibody has been cited in at least 17 peer reviewed research publications in journals of high repute. We have only one Niemann-Pick type C2 antibody with catalog # H00010577-D01P, but this antibody is yet to be established for IHC application. However, if you would like to try this antibody for IHC-P application you would qualify for our Innovators Reward program. Through this program if you complete an online review with image, detailing your positive or negative results we will send you a discount voucher for 100% of the purchase price of the reviewed product. Read more about Innovators Reward Program.
-
Q: We have an inquiry of your product Cat. ID # NB400-148. Our customer would like to know the immunogen sequence of this product. If you cannot provide the detail, would you please check if this antibody covers amino acids 600-800?
A: This antibody was made to a sequence found within amino acids 1230-1278.
-
Q: Have you received any complaints on these lots F1 and G1 of NB400-148?
A: I have looked through our complaints and spoke to our lab manager, and we have not had any recent reported problems with these lots. These lots do happen to be approximately two years old, so it may be that they have lost reactivity over this time. Our antibody products are guaranteed for 6 months from date of receipt.
-
Q: I want to purchase NPC1 antibody from your company and the catalog is NB400-148. Could you please tell me in which precise position the antibody binds of the NPC1 protein?
A: NB400-148 binds between amino acids 1178 and 1278 of human Niemann-Pick C.
-
Q: I'm looking for a Niemann-Pick type1 or 2 antibody to stain mouse heart section embedded in paraffin. I would like to label these sections with another primary antibody to show colocalisation of my protein of interest. I would like to know if you have a rabbit anti-Niemann-Pick antibody that works well for fluorescence immunohistochemistry?
A: Niemann-Pick C1 Antibody (NB400-148) (0.1 ml) is one of our best sellers and this antibody has been cited in at least 17 peer reviewed research publications in journals of high repute. We have only one Niemann-Pick type C2 antibody with catalog # H00010577-D01P, but this antibody is yet to be established for IHC application. However, if you would like to try this antibody for IHC-P application you would qualify for our Innovators Reward program. Through this program if you complete an online review with image, detailing your positive or negative results we will send you a discount voucher for 100% of the purchase price of the reviewed product. Read more about Innovators Reward Program.
-
Q: We have an inquiry of your product Cat. ID # NB400-148. Our customer would like to know the immunogen sequence of this product. If you cannot provide the detail, would you please check if this antibody covers amino acids 600-800?
A: This antibody was made to a sequence found within amino acids 1230-1278.