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Nitrotyrosine Alexa Fluor® 647-conjugated Antibody

Catalog #: FAB3248R Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB3248R-100UG

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Nitrotyrosine Alexa Fluor® 647-conjugated Antibody Summary

Specificity
Detects Nitrotyrosine adducts on proteins in Western blots. It does not cross-react with phosphotyrosine or 4‑hydroxynonenal adducts. Unfixed cells, tissues, and proteins can be treated with Peroxynitrite (Catalog #
Source
Monoclonal Mouse IgG3 Clone # 306507
Purification
Protein A or G purified
Immunogen
Nitrotyrosine-modified KLH
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Label
Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm)
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Nitrotyrosine

3-Nitrotyrosine is formed when tyrosine is reacted with peroxynitrite. Since peroxynitrite is formed from nitric oxide and superoxide anion, nitrotyrosine adducts on proteins have been used as markers of oxidative cellular damage and macrophage activation. Elevated nitrotyrosine immunoreactivity has been found in inflammation, osteoarthritis, neurodegenerative diseases, and ischemic damage to the heart and brain.

Alternate Names
Nitrotyrosine

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