Nitrotyrosine Antibody

Catalog # Availability Size / Price Qty
MAB3248
MAB3248-SP
Nitrotyrosine Antibody in Western Blot
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Product Details
Citations (2)
FAQs
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Nitrotyrosine Antibody Summary

Specificity
Detects Nitrotyrosine adducts on proteins in Western blots. It does not cross-react with phosphotyrosine or 4‑hydroxynonenal adducts. Cells, tissues, and proteins can be treated with Peroxynitrite (Catalog # AR006) for use as positive controls with this antibody.
Source
Monoclonal Mouse IgG3 Clone # 306507
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Nitrotyrosine-modified KLH
Formulation
Lyophilized from a 0.2 μm filtered solution in TBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Nitrotyrosine by Western Blot. View Larger

Detection of Nitrotyrosine by Western Blot. Western blot shows lysates of NIH-3T3 mouse embryonic fibroblast cell line untreated (-) or treated (+) with 3 mM peroxynitrite, 3 mM inactivated peroxynitrite, or 100 µM peroxyvanadate for 1 hour and recombinantE. coliDNAK treated with 1 mM 4-hydroxynonenal or 1 mM peroxyynitrite for 1 hour. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Nitrotyrosine Monoclonal Antibody (Catalog # MAB3248), followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). The lysates were also probed with Phospho-Tyrosine Monoclonal Antibody (Catalog # MAB1676). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Immunocytochemistry Nitrotyrosine in Human PBMCs. View Larger

Nitrotyrosine in Human PBMCs. Nitrotyrosine was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using 25 µg/mL Mouse Anti-Nitrotyrosine Monoclonal Antibody (Catalog # MAB3248) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained (green). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Nitrotyrosine

3-Nitrotyrosine is formed when tyrosine is reacted with peroxynitrite. Since peroxynitrite is formed from nitric oxide and superoxide anion, nitrotyrosine adducts on proteins have been used as markers of oxidative cellular damage and macrophage activation. Elevated nitrotyrosine immunoreactivity has been found in inflammation, osteoarthritis, neurodegenerative diseases, and ischemic damage to the heart and brain.

Alternate Names
Nitrotyrosine

Product Datasheets

Citations for Nitrotyrosine Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Deleterious effects of phosphate on vascular and endothelial function via disruption to the nitric oxide pathway
    Authors: Alan G Jardine
    Nephrol. Dial. Transplant., 2016;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Age-related retinopathy in NRF2-deficient mice.
    Authors: Zhao Z, Chen Y, Wang J
    PLoS ONE, 2011;6(4):e19456.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr

FAQs

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Cell and Tissue Staining Kits

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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