Nitrotyrosine Antibody (EM-30) - BSA Free

Novus Biologicals | Catalog # NBP1-96130

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Mouse, Non-species specific

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # EM-30

Format

BSA Free
View all Nitrotyrosine Primary Antibodies »

Product Specifications

Immunogen

NO2-Tyr-CH2-Thyroglobulin

Reactivity Notes

This antibody reacts to large number of species. Use in Mouse reported in secitific publication PMID: 32793221

Specificity

The antibody EM-30 detects nitrotyrosine group in biological material. It can be used as a marker for peroxynitrite formation in particular tissues

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Nitrotyrosine Antibody (EM-30) - BSA Free

Immunohistochemistry-Frozen: Nitrotyrosine Antibody (EM-30) - BSA Free [NBP1-96130]

Immunohistochemistry-Frozen: Nitrotyrosine Antibody (EM-30) - BSA Free [NBP1-96130]

Immunohistochemistry-Frozen: Nitrotyrosine Antibody (EM-30) [NBP1-96130] - Human tumoral colon epithelium with anti-nitrotyrosine antibody (EM-30).

Applications for Nitrotyrosine Antibody (EM-30) - BSA Free

Application
Recommended Usage

Immunohistochemistry-Frozen

10 ug/ml

Immunohistochemistry-Paraffin

10 ug/ml
Application Notes
This antibody I useful for Western Blot (Positive control:NO2-BSA, NO2-HS) Immunohistochemistry-paraffin( Positive tissue: colon cancer epithelium, prostate hyperplasia)and Immunohistochemistry-frozen (Positive tissue: colon cancer epithelium, prostate hyperplasia) Use in ICC/IF reported in secitific publication PMID: 32793221

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

Phosphate buffered saline (PBS), pH 7.4

Format

BSA Free

Preservative

15mM Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Nitrotyrosine

Nitrotyrosine can be detected in proteins from a variety of tissues usually in association with pathological conditions. Reaction of nitric oxide with superoxide produces peroxynitrite, which can undergo heterolytic cleavage into nitronium and hydroxyl ions. Nitration of tyrosine residues by nitronium ion forms nitrotyrosine groups in the respective proteins. Nitrotyrosine is thus a marker for inflammation-associated tissue damage.

Alternate Names

Nitrotyrosine

Additional Nitrotyrosine Products

Product Documents for Nitrotyrosine Antibody (EM-30) - BSA Free

Certificate of Analysis

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Product Specific Notices for Nitrotyrosine Antibody (EM-30) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Nitrotyrosine Antibody (EM-30) - BSA Free

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Protocols

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