Nitrotyrosine Antibody

Novus Biologicals | Catalog # NB100-2899

Novus Biologicals
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Key Product Details

Species Reactivity

Rat, Non-species specific

Applications

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, ELISA

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all Nitrotyrosine Primary Antibodies »

Product Specifications

Immunogen

KLH-nitrotyrosine

Reactivity Notes

Rat reactivity reported from a verified customer review.

Specificity

The nitrotyrosine antibody reacts with free and bound nitrotyrosine. There is no cross-reactivity with free and bound tyrosine.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Nitrotyrosine Antibody

Immunohistochemistry-Frozen: Nitrotyrosine Antibody [NB100-2899]

Immunohistochemistry-Frozen: Nitrotyrosine Antibody [NB100-2899]

Immunohistochemistry-Frozen: Nitrotyrosine Antibody [NB100-2899] - Rat left ventricle embedded in OCT, flash-frozen in liquid nitrogen and stored at -80C. 8 um sections cut using a cryostat, fixed with ice-cold acetone, blocked using 2% BSA in PBS with 5% normal goat serum, followed by incubation with nitrotyrosine antibody at 1:50 dilution overnight at 4C. Secondary antibody was goat anti-rabbit Alexa Fluor 488 at 1:250 dilution for 1hr at RT. IHC-Fr image submitted by a verified customer review.

Applications for Nitrotyrosine Antibody

Application
Recommended Usage

ELISA

1:1 000 - 1:20 000

Western Blot

1:200 - 1:2000
Application Notes
Nitrotyrosine antibody validated for IHC-Fr from a verified customer review.

Reviewed Applications

Read 1 review rated 5 using NB100-2899 in the following applications:

Formulation, Preparation, and Storage

Purification

Unpurified

Reconstitution

Reconstitute in distilled water.

Formulation

Antiserum

Preservative

No Preservative

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Nitrotyrosine

Protein tyrosine nitration results in a post-translational modification that is increasingly receiving attention as an important component of nitric oxide signaling (2). While multiple nonenzymatic mechanisms are known to be capable of producing nitrated tyrosine residues, most tyrosine nitration events involve catalysis by metalloproteins such as myeloperoxidase, eosinophilperoxidase (3), myoglobin, the cytochrome P-450s, superoxide dismutase and prostacyclin synthase. Nitrotyrosine may also serve as a biomarker for the effects of reactive nitrogen oxides, based on tyrosine residues becoming nitrated in proteins at sites of inflammation induced tissue injury (1). The presence of nitro tyrosine-containing proteins therefore has shown high correlation to disease states such as atherosclerosis, Alzheimer's disease, Parkinson's disease and amyotrophic lateral sclerosis (4).

Alternate Names

Nitrotyrosine

Additional Nitrotyrosine Products

Product Documents for Nitrotyrosine Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Nitrotyrosine Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for Nitrotyrosine Antibody (1)

5 out of 5
1 Customer Rating
5 Stars
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4 Stars
0%
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2 Stars
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1 Stars
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Customer Images

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  • Nitrotyrosine Antibody
    Name: Anonymous
    Application: Immunohistochemistry-Frozen
    Sample Tested: Adult heart
    Species: Rat
    Verified Customer | Posted 08/19/2019
    rat left ventricle embedded in OCT, then flash-frozen in liquid nitrogen and stored at -80C. 8 um sections cut using a cryostat. sections fixed with ice-cold acetone, blocked using 2%BSA in PBS with 5% normal goat serum, followed by incubation with 1:50 dilution overnight at 4C. Secondary antibody was goat anti-rabbit Alexa Fluor 488 from ThermoFisher Scientific at 1:250 dilution for 1hr at RT.
    Nitrotyrosine Antibody NB100-2899

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Protocols

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FAQs

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