NK1R Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NB300-101

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Guinea Pig

Cited:

Human, Mouse, Rat, Guinea Pig

Predicted:

Canine (92%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all NK1R Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to the C-terminus of rat Neurokinin 1 Receptor, conjugated to bovine thyroglobulin. [UniProt# P14600]

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: canine (92%)

Localization

In immunohistochemical experiments, plasma membrane staining is observed.

Specificity

NB300-101 is specific to the NK-1 receptor. Specificity was verified by the localization of immunohistochemical staining with NB300-101 in the neurons of mouse brain tissue.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NK1R Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: NK1R Antibody [NB300-101]

Immunocytochemistry/ Immunofluorescence: NK1R Antibody [NB300-101]

Immunocytochemistry/Immunofluorescence: Neurokinin 1 Receptor Antibody [NB300-101] - IF analysis of Neurokinin 1 Receptor in rat brain tissue. Image courtesy of anonymous customer product review.
Immunohistochemistry: NK1R Antibody [NB300-101]

Immunohistochemistry: NK1R Antibody [NB300-101]

Immunohistochemistry: Neurokinin 1 Receptor Antibody [NB300-101] - Immunohistochemical analysis of Neurokinin 1 Receptor in mouse brain (selected cells, axon & dendrites) using NB300-101.
Immunocytochemistry/ Immunofluorescence: NK1R Antibody [NB300-101]

Immunocytochemistry/ Immunofluorescence: NK1R Antibody [NB300-101]

Immunocytochemistry/Immunofluorescence: Neurokinin 1 Receptor Antibody [NB300-101] - Confocal image of SPR reactivity using polyclonal anti-NK-1 Receptor (NB 300-101) in a lamina III neuron in the spinal cord of a rat.
Immunocytochemistry/ Immunofluorescence: NK1R Antibody [NB300-101]

Immunocytochemistry/ Immunofluorescence: NK1R Antibody [NB300-101]

Immunocytochemistry/Immunofluorescence: Neurokinin 1 Receptor Antibody [NB300-101] - IF analysis of Neurokinin 1 Receptor in rat brain tissue. Image courtesy of anonymous customer product review.

Applications for NK1R Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50

Immunohistochemistry

1:1000

Immunohistochemistry-Frozen

1:1000

Immunohistochemistry-Paraffin

reported in scientific literature (PMID 23792204)

Western Blot

1:100-1:2000

Reviewed Applications

Read 2 reviews rated 4 using NB300-101 in the following applications:

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Whole antisera

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: NK1R

Neurokinin 1 receptor (NK1R) is a Gq-coupled receptor multi-pass membrane protein localized in the cell membranes of a variety of cells including CNS/PNS neurons, immune cells, endocrine and exocrine glands, smooth muscle cells etc. NK1R is an endogenous receptor for substance P (neuropeptide that belongs to tachykinin neuropeptide family), and is a highly conserved receptor which binds high affinity ligands. NK1R couples to a subgroup of G-proteins, and interacts with ARRB1 as well. NK1R-SP interactions lead to activation of phospholipase CB (PLCB), and results in a transient increase in intracellular inositol 1,4,5 triphosphate (IP3) diacyl-glycerol and increased cytosolic Ca2+concentration. This interaction is involved in pain transmission, vasodilatation, connective-tissue cell proliferation, neuroimmune modulation and exocrine (e.g. sialogogue) as well as endocrine secretions.

Long Name

Neurokinin-1 Receptor

Alternate Names

SPR, TAC1R, TACR1

Entrez Gene IDs

6869 (Human); 24807 (Rat)

Gene Symbol

TACR1

UniProt

P14600 (Rat)

Additional NK1R Products

Product Documents for NK1R Antibody - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for NK1R Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for NK1R Antibody - Azide and BSA Free

Customer Reviews for NK1R Antibody - Azide and BSA Free (2)

4 out of 5
2 Customer Ratings
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100%
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Customer Images

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  • Name: Li-Hsien Lin
    Application: Immunofluorescence
    Sample Tested: Rat brain tissue
    Species: Rat
    Verified Customer | Posted 12/20/2010
    NK1R Antibody - Azide and BSA Free NB300-101
  • Name: Li-Hsien Lin
    Application: Immunofluorescence
    Sample Tested: rat brain sections
    Species: Rat
    Verified Customer | Posted 09/09/2010
    NK1R Antibody - Azide and BSA Free NB300-101

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Protocols

View specific protocols for NK1R Antibody - Azide and BSA Free (NB300-101):


Immunohistochemistry

1) Perfuse rat through the ascending aorta with 500 ml. of 0.1 M phosphate buffered saline (PBS) (pH 7.4 @ 4 degrees C), followed by 750 ml. of PBS containing 4% formaldehyde and 12.5% picric acid (pH 6.9, 4 degrees C). Tissue of interest is then dissected and post-fixed in PBS containing 4% formaldehyde and 12.5% picric acid (pH 6.9 at 4 degrees C) for at least 4 hours, followed by fixation in PBS containing 30% sucrose (pH 7.4, 4 degrees C) for at least 24 hours. Until the tissue sinks.

2) Cut fixed sections at 60 um using a sliding microtome.

3) If using culture cells, remove media and perform 2-3 washed in PBS (pH 7.4) -use same protocol without agitation.

4) Place tissue section in microcentrifuge tubes containing 1 ml. Of PBS, pH 7.4 as they are being cut. Wash sections (in PBS) on an upright rotator for 10-15 minutes.

5) Remove PBS and add 1 ml. of blocking solution (PBS + 1% normal donkey serum (NDS) + 0.3% triton X-100).

6) Incubate tissue sections in blocking solution for 30 minutes at room temperature (RT) on rotator.

7) Remove blocking solution and add anti-neurokinin-1 receptor or anti-Neurokinin-3 receptor(NB 300-101/NB 100-102) antibody +PBS + 1% NDS + 0.3% triton X-100 + NB 300-101/102 @ 1:100~1:1000

8) Incubate overnight on rotator at RT.

9) Remove primary antisera and perform 3 x 10 minute PBS washes (1ml volume) on rotator.

10) Add 1 ml. of secondary antibody and incubate for 2 hours at RT on rotator.
-PBS + 1% NDS + 0.3% triton X 100 + anti-rabbit Cy3 (Jackson Immunoresearch Labs) at 1:600

11) Perform 3 X 10 minute PBS washes (1 ml volume) on rotator

12) Mount tissue sections on gelatin coated slides and allow tissue to dry

13) Run slides through alcohol gradients (70%, 90%, 100%, xylene) leaving slide in each alcohol and xylene for 2 minutes

14) Coverslip with DPX mountant (FLUKA) ** Note: 24 well plates may be substituted for microcentrifuge
tubes. Use flat top bench rotator instead of upright rotator.

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FAQs for NK1R Antibody - Azide and BSA Free

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  • Q: Question about the Neurokinin 1 Receptor Antibody (NB300-101):why were the NK-1R in WB reported with different molecular mass(46kd, 50kd or about 70kd?)in publication 1,2 and 3? which is right?

    A:

    The theoretical MW of this protein is about 46KDa. Please see this reference for P25103 (https://www.uniprot.org/uniprotkb/P25103/entry). This is a protein database. The above MW weight is only theoretical and it can be expected that the MW differs between each experiment. The researcher who experienced this protein to be at 70KDa may not have reduced their protein enough which could have led to a protein complex, resulting in a larger MW.

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