NKX2.2 Antibody (CL11380) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-44133

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL11380

Format

Azide and BSA Free
View all NKX2.2 Primary Antibodies »

Product Specifications

Immunogen

This antibody was generated using a recombinant protein sequence of O95096, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for NKX2.2 Antibody (CL11380) - Azide and BSA Free

NKX2.2 Antibody (CL11380) - Azide and BSA Free Immunohistochemistry-Paraffin: NKX2.2 Antibody [NBP3-44133]

Immunohistochemistry-Paraffin: NKX2.2 Antibody [NBP3-44133]

Analysis in human cerebral cortex and liver tissues using NBP3-44133 antibody. Corresponding NKX2.2 RNA-seq data are presented for the same tissues.
NKX2.2 Antibody (CL11380) - Azide and BSA Free Immunohistochemistry-Paraffin: NKX2.2 Antibody [NBP3-44133]

Immunohistochemistry-Paraffin: NKX2.2 Antibody [NBP3-44133]

Staining of human Cerebral cortex shows strong nuclear positivity in neuronal and glial cells.
NKX2.2 Antibody (CL11380) - Azide and BSA Free Immunohistochemistry-Paraffin: NKX2.2 Antibody [NBP3-44133]

Immunohistochemistry-Paraffin: NKX2.2 Antibody [NBP3-44133]

Staining of human Pancreas shows strong nuclear positivity in islets of Langerhans.
NKX2.2 Antibody (CL11380) - Azide and BSA Free Immunohistochemistry-Paraffin: NKX2.2 Antibody [NBP3-44133]

Immunohistochemistry-Paraffin: NKX2.2 Antibody [NBP3-44133]

Staining of human Liver shows no positivity in hepatocytes as expected.
NKX2.2 Antibody (CL11380) - Azide and BSA Free Immunohistochemistry-Paraffin: NKX2.2 Antibody [NBP3-44133]

Immunohistochemistry-Paraffin: NKX2.2 Antibody [NBP3-44133]

Staining of human Stomach shows strong nuclear positivity in enteroendocrine cells.
NKX2.2 Antibody (CL11380) - Azide and BSA Free Western Blot: NKX2.2 Antibody [NBP3-44133]

Western Blot: NKX2.2 Antibody [NBP3-44133]

Analysis in human cell line U-138MG.

Applications for NKX2.2 Antibody (CL11380) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: NKX2.2

Nkx2.2, or Homeobox protein Nkx-2.2, consists of a 273 amino acid isoform that is 30 kDa, and is involved in controlling and maintaining the expression of genes within pancreatic endocrine cells and axons. Current research is being conducted on the relationship between Nkx2.2 and a variety of diseases and disorders, including glioma, neuronitis, maturity-onset diabetes, pancreatitis, Partington syndrome, sarcoma, gastrointestinal neuroendocrine tumor, acute lymphoblastic leukemia, multiple sclerosis, and carcinoid tumors. The protein has been linked to the Wnt, Hedgehog, and notch pathways, as well as regulation of beta-cell development and gene expression. NKx2.2 interacts with several other proteins, including OLIG2, HDAC1, SIN3A, FOXA2, and FOXO1.

Long Name

NK2 Homeobox 2

Alternate Names

NKX2B

Gene Symbol

NKX2-2

Additional NKX2.2 Products

Product Documents for NKX2.2 Antibody (CL11380) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for NKX2.2 Antibody (CL11380) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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