NONO Antibody

Novus Biologicals | Catalog # NB100-1556

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse

Predicted:

Orangutan (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all NONO Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residues 350 and 400 of human Non-POU Domain Containing, Octamer-binding using the numbering given in entry NP_031389.3 (GeneID 4841).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

54 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NONO Antibody

Western Blot: NONO Antibody [NB100-1556]

Western Blot: NONO Antibody [NB100-1556]

Western Blot: NONO Antibody [NB100-1556] - B-cell whole cell lysate 40ug. Image submitted by a verified customer review
Immunocytochemistry: NONO Antibody [NB100-1556]

Immunocytochemistry: NONO Antibody [NB100-1556]

Immunocytochemistry: NONO Antibody [NB100-1556] - NONO in human fibroblasts at 1:200 dilution in 1X PBS/1% BSA/0.3% Triton X-100, 16 hours 4C. Image submitted by a verified customer review.
Immunohistochemistry: NONO Antibody [NB100-1556]

Immunohistochemistry: NONO Antibody [NB100-1556]

Immunohistochemistry: NONO Antibody [NB100-1556]
Western Blot: NONO Antibody [NB100-1556]

Western Blot: NONO Antibody [NB100-1556]

Western Blot: NONO Antibody [NB100-1556] - Detection of Human NONO on HeLa whole cell lysate (5, 15, and 50 microgram) and 293T cell lystate (50 microgram) using NB100-1556. NONO was also immunoprecipitated using anti-NONO antibody NB100-1554 or NB100-1555 at 3 microg/mg lysate.
Western Blot: NONO Antibody [NB100-1556]

Western Blot: NONO Antibody [NB100-1556]

Western Blot: NONO Antibody [NB100-1556] - Whole cell lysate NIH3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells.
Immunohistochemistry-Paraffin: NONO Antibody [NB100-1556]

Immunohistochemistry-Paraffin: NONO Antibody [NB100-1556]

Immunohistochemistry-Paraffin: NONO Antibody [NB100-1556] - Sample: FFPE section of human breast carcinoma (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti- NONO used at a dilution of 1:1,000 (0.2 ug/ml) and 1:200 (1ug/ml). Detection: DAB
Immunohistochemistry-Paraffin: NONO Antibody [NB100-1556]

Immunohistochemistry-Paraffin: NONO Antibody [NB100-1556]

Immunohistochemistry-Paraffin: NONO Antibody [NB100-1556] - Affinity purified rabbit anti-NONO used at a dilution of 1:1,000 (0.2 ug/ml).

Applications for NONO Antibody

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:1:000

Immunohistochemistry-Paraffin

1:200 - 1:1:000

Immunoprecipitation

1-4 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
NONO antibody validated for ICC/IF, WB from verified customer reviews. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Reviewed Applications

Read 2 reviews rated 4.5 using NB100-1556 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: NONO

Non-POU-domain-containing octamer binding protein (NONO) is a member of the DBHS (drosophila behavior, human splicing) domain-containing family and is an RNA- and DNA- binding protein. NONO and other DBHS domain-containing proteins are multifunctional and are reported to be involved in transcriptional regulation, mRNA processing, and DNA non-homologous end joining (NHEJ). NONO functions as a coregulator of the androgen receptor (AR) and also regulates cAMP transcriptional activity by interacting with gene promoter elements. NONO is also involved in pre-mRNA splicing through an interaction with U5 snRNA and can stimulate DNA nonhomologous end joining (NHEJ) through the interaction with ku70/G22p and ku80/XRCC5 dimers. Alternate names for NONO include 54 kDa nuclear RNA- and DNA-binding protein, p54 (nrb), 55 kDa nuclear protein, NMT55, DNA-binding p52/p100 complex, NRB54, P54, and P54NRB.

Alternate Names

NMT5552 kDa subunit, non-POU domain containing, octamer-binding, NRB54non-POU-domain-containing, octamer-binding, p54(nrb)

Entrez Gene IDs

4841 (Human)

Gene Symbol

NONO

UniProt

Q15233 (Human)

Additional NONO Products

Product Documents for NONO Antibody

Certificate of Analysis

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Product Specific Notices for NONO Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for NONO Antibody (2)

4.5 out of 5
2 Customer Ratings
5 Stars
50%
4 Stars
50%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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Customer Images

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  • NONO Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: B-cell whole cell lysate 40ug
    Species: Human
    Verified Customer | Posted 03/09/2018
    BIOSCARF
    NONO Antibody NB100-1556
  • NONO Antibody
    Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: fibroblasts
    Species: Human
    Verified Customer | Posted 01/11/2018
    1:200 in 1X PBS/1% BSA/0.3% Triton X-100, 16 hours 4C.
    NONO Antibody NB100-1556

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Protocols

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FAQs

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