Q: Hello, does this product (NB300-251) only recognize the cleavage Notch1?

A: This antibody with recognize full length Notch1 if it is in the mix, as well as the cleaved product.

Notch-1 Antibody (7L3V4)

Novus Biologicals | Catalog # NBP3-15658

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 7L3V4 expressed in HEK293

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Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 2456-2555 of human Notch-1 (P46531). ILPQESPALPTSLPSSLVPPVTAAQFLTPPSQHSYSSPVDNTPSHQLQVPEHPFLTPSPESPDQWSSSSPHSNVSDWSEGVSSPPTSMQSQIARIPEAFK

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Notch-1 Antibody (7L3V4)

Western Blot: Notch-1 Antibody (7L3V4) [NBP3-15658]

Western Blot: Notch-1 Antibody (7L3V4) [NBP3-15658] - Western blot analysis of extracts of various cell lines, using Notch-1 antibody (NBP3-15658) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Immunoprecipitation: Notch-1 Antibody (7L3V4) [NBP3-15658]

Immunoprecipitation: Notch-1 Antibody (7L3V4) [NBP3-15658] - Immunoprecipitation analysis of 200ug extracts of 293T cells using 3ug Notch-1 antibody (NBP3-15658). Western blot was performed from the immunoprecipitate using Notch-1 antibody (NBP3-15658) at a dilition of 1:1000.

Applications for Notch-1 Antibody (7L3V4)

Application

Recommended Usage

Immunoprecipitation

1:50 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Notch-1

Notch is synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase (S1 cleavage) in the trans-golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin-dependent gamma-secretase (S3 cleavage) to release the intracellular domain (NICD) from the membrane which then translocates to the nucleus to activate transcription of downstream genes.

Alternate Names

Notch1, TAN1

Gene Symbol

NOTCH1

Additional Notch-1 Products

Product Documents for Notch-1 Antibody (7L3V4)

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Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Notch-1 Antibody (7L3V4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Astrocytes

Hypoxia Inducible Factors (HIFs)

Neural Crest Cell Markers

Notch Pathway

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Notch-1 Antibody (7L3V4)

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Q: Hello, does this product (NB300-251) only recognize the cleavage Notch1?

A: This antibody with recognize full length Notch1 if it is in the mix, as well as the cleaved product.