Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

DyLight 350 (Excitation = 353 nm, Emission = 432 nm)

Antibody Source

Polyclonal Sheep IgG
View all Notch-1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Notch-1
Ala19-Gln526
Accession # P46531

Specificity

Detects human Notch-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross‑reactivity with with recombinant mouse Notch-1 (aa 19‑526) and recombinant rat Notch-1 (aa 20‑488) is observed and less than 1% cross-reactivity with recombinant human (rh) Notch-2 and rhNotch-3 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Applications for Notch-1 Antibody [DyLight 350]

Application
Recommended Usage

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Notch-1

Notch is synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase (S1 cleavage) in the trans-golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin-dependent gamma-secretase (S3 cleavage) to release the intracellular domain (NICD) from the membrane which then translocates to the nucleus to activate transcription of downstream genes.

Alternate Names

Notch1, TAN1

Gene Symbol

NOTCH1

Additional Notch-1 Products

Product Documents for Notch-1 Antibody [DyLight 350]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Notch-1 Antibody [DyLight 350]



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs for Notch-1 Antibody [DyLight 350]

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  • Q: Hello, does this product (NB300-251) only recognize the cleavage Notch1?

    A: This antibody with recognize full length Notch1 if it is in the mix, as well as the cleaved product.

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